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Electron microscopy of native cartilage oligomeric matrix protein purified from the Swarm rat chondrosarcoma reveals a five-armed structure

Mörgelin, Matthias LU ; Heinegård, Dick LU ; Engel, Jürgen and Paulsson, Mats (1992) In Journal of Biological Chemistry 267(9). p.6137-6141
Abstract
Cartilage oligomeric matrix protein was isolated in the native state from the Swarm rat chondrosarcoma. A crucial step was its selective extraction with EDTA-containing buffer. The purified protein was subjected to electron microscopy using rotary shadowing and negative stain. The images allowed the construction of a structural model. The bouquet-like protein consists of five 28-nm-long arms containing a peripheral globular domain, a flexible strand, and a central assembly domain, where the five arms meet in a cylindrical structure.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
267
issue
9
pages
6137 - 6141
publisher
ASBMB
external identifiers
  • pmid:1556122
  • scopus:0026703257
ISSN
1083-351X
language
English
LU publication?
yes
id
67f156bb-ecc6-4f9c-89ac-ecd0c651d199 (old id 1106697)
alternative location
http://www.jbc.org/cgi/reprint/267/9/6137
date added to LUP
2008-08-08 13:50:15
date last changed
2017-10-22 03:37:27
@article{67f156bb-ecc6-4f9c-89ac-ecd0c651d199,
  abstract     = {Cartilage oligomeric matrix protein was isolated in the native state from the Swarm rat chondrosarcoma. A crucial step was its selective extraction with EDTA-containing buffer. The purified protein was subjected to electron microscopy using rotary shadowing and negative stain. The images allowed the construction of a structural model. The bouquet-like protein consists of five 28-nm-long arms containing a peripheral globular domain, a flexible strand, and a central assembly domain, where the five arms meet in a cylindrical structure.},
  author       = {Mörgelin, Matthias and Heinegård, Dick and Engel, Jürgen and Paulsson, Mats},
  issn         = {1083-351X},
  language     = {eng},
  number       = {9},
  pages        = {6137--6141},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Electron microscopy of native cartilage oligomeric matrix protein purified from the Swarm rat chondrosarcoma reveals a five-armed structure},
  volume       = {267},
  year         = {1992},
}