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Evaluation of ethanol effects on PLC signal transduction pathways using cell lines of neuronal origin

Alling, Christer LU ; Bergman, O; Larsson, Christer LU and Simonsson, Per LU (1993) In Alcohol and alcoholism. Supplement 2. p.295-299
Abstract
Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA... (More)
Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA response after 40 minutes in SH-SY5Y cells, an effect that could be mimicked through protein kinase C stimulation by phorbol esters. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Alcohol and alcoholism. Supplement
volume
2
pages
295 - 299
publisher
Pergamon
external identifiers
  • pmid:7748314
  • scopus:0027902710
ISSN
1358-6173
language
English
LU publication?
yes
id
4be1e77e-82f3-409d-ab93-9d89a0cf24ce (old id 1106956)
date added to LUP
2008-07-30 08:35:58
date last changed
2017-08-06 04:31:00
@article{4be1e77e-82f3-409d-ab93-9d89a0cf24ce,
  abstract     = {Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA response after 40 minutes in SH-SY5Y cells, an effect that could be mimicked through protein kinase C stimulation by phorbol esters.},
  author       = {Alling, Christer and Bergman, O and Larsson, Christer and Simonsson, Per},
  issn         = {1358-6173},
  language     = {eng},
  pages        = {295--299},
  publisher    = {Pergamon},
  series       = {Alcohol and alcoholism. Supplement},
  title        = {Evaluation of ethanol effects on PLC signal transduction pathways using cell lines of neuronal origin},
  volume       = {2},
  year         = {1993},
}