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Comparative cytogenetic and DNA flow cytometric analysis of 150 bone and soft-tissue tumors

Mandahl, Nils LU ; Baldetorp, Bo LU ; Fernö, Mårten LU ; Åkerman, Måns LU ; Rydholm, Anders LU ; Heim, Sverre LU ; Willén, Helena; Killander, Dick LU and Mitelman, Felix LU (1993) In International Journal of Cancer 53(3). p.358-364
Abstract
Samples from 48 benign and 102 malignant bone and soft-tissue tumors were analyzed cytogenetically and by DNA flow cytometry. Clonal chromosome abnormalities were found in 82 tumors and normal karyotypes in 68; 61 tumors were DNA-non-diploid and 89 were diploid. The cytogenetically abnormal tumors were used for comparison between the 2 types of investigation; 45 of these tumors were DNA-diploid and 37 were DNA-non-diploid. There was, with few exceptions, good correspondence between the quantitative estimates of genomic changes by the 2 methods, indicating that the cells cytogenetically analyzed from short-term cultures are representative of the in vivo cell populations. Discrepancies were primarily found in cases with indexes above 1.5, in... (More)
Samples from 48 benign and 102 malignant bone and soft-tissue tumors were analyzed cytogenetically and by DNA flow cytometry. Clonal chromosome abnormalities were found in 82 tumors and normal karyotypes in 68; 61 tumors were DNA-non-diploid and 89 were diploid. The cytogenetically abnormal tumors were used for comparison between the 2 types of investigation; 45 of these tumors were DNA-diploid and 37 were DNA-non-diploid. There was, with few exceptions, good correspondence between the quantitative estimates of genomic changes by the 2 methods, indicating that the cells cytogenetically analyzed from short-term cultures are representative of the in vivo cell populations. Discrepancies were primarily found in cases with indexes above 1.5, in which the DNA index was higher than the chromosome index. The chromosome analysis suggested that skewed stemline (G0/G1) peaks in the diploid region in DNA histograms indicate the presence of cell populations with small net quantitative genomic changes, although not all such populations were detected by DNA flow cytometric analysis. The view that one of the peaks in bimodal stemline DNA histograms with narrow peaks represents a non-diploid cell population was also corroborated. On average, the cell populations giving rise to double stemlines in DNA histograms showed quantitatively larger genomic changes than those that gave rise to broad or skewed diploid G0/G1 peaks. The findings indicate that these histogram profiles are not artifactual but reflect chromosomal changes in the tumor parenchyma. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Cancer
volume
53
issue
3
pages
358 - 364
publisher
John Wiley & Sons
external identifiers
  • pmid:8428789
  • scopus:0027513578
ISSN
0020-7136
DOI
10.1002/ijc.2910530303
language
English
LU publication?
yes
id
2f529f7e-16c4-4f66-9dba-9a68878799fb (old id 1107055)
date added to LUP
2008-07-30 10:07:15
date last changed
2017-07-30 03:42:13
@article{2f529f7e-16c4-4f66-9dba-9a68878799fb,
  abstract     = {Samples from 48 benign and 102 malignant bone and soft-tissue tumors were analyzed cytogenetically and by DNA flow cytometry. Clonal chromosome abnormalities were found in 82 tumors and normal karyotypes in 68; 61 tumors were DNA-non-diploid and 89 were diploid. The cytogenetically abnormal tumors were used for comparison between the 2 types of investigation; 45 of these tumors were DNA-diploid and 37 were DNA-non-diploid. There was, with few exceptions, good correspondence between the quantitative estimates of genomic changes by the 2 methods, indicating that the cells cytogenetically analyzed from short-term cultures are representative of the in vivo cell populations. Discrepancies were primarily found in cases with indexes above 1.5, in which the DNA index was higher than the chromosome index. The chromosome analysis suggested that skewed stemline (G0/G1) peaks in the diploid region in DNA histograms indicate the presence of cell populations with small net quantitative genomic changes, although not all such populations were detected by DNA flow cytometric analysis. The view that one of the peaks in bimodal stemline DNA histograms with narrow peaks represents a non-diploid cell population was also corroborated. On average, the cell populations giving rise to double stemlines in DNA histograms showed quantitatively larger genomic changes than those that gave rise to broad or skewed diploid G0/G1 peaks. The findings indicate that these histogram profiles are not artifactual but reflect chromosomal changes in the tumor parenchyma.},
  author       = {Mandahl, Nils and Baldetorp, Bo and Fernö, Mårten and Åkerman, Måns and Rydholm, Anders and Heim, Sverre and Willén, Helena and Killander, Dick and Mitelman, Felix},
  issn         = {0020-7136},
  language     = {eng},
  number       = {3},
  pages        = {358--364},
  publisher    = {John Wiley & Sons},
  series       = {International Journal of Cancer},
  title        = {Comparative cytogenetic and DNA flow cytometric analysis of 150 bone and soft-tissue tumors},
  url          = {http://dx.doi.org/10.1002/ijc.2910530303},
  volume       = {53},
  year         = {1993},
}