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Production of alpha-1-antichymotrypsin by PSA-containing cells of human prostate epithelium

Bjartell, Anders LU ; Björk, Thomas LU ; Matikainen, Maria-Terttu; Abrahamsson, Per-Anders LU ; di Sant'Agnese, Anthony and Lilja, Hans LU (1993) In Urology 42(5). p.502-510
Abstract
Prostate-specific antigen (PSA) is a chymotrypsin-like serine protease exclusively produced by the prostate epithelium, and abundant in seminal fluid. In serum, PSA is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. Since the molecular basis for this is unclear, we have investigated whether or not ACT may be produced in the prostate gland. Immunocytochemistry, using either monoclonal or polyclonal IgGs, demonstrated specific immunostaining for ACT in normal PSA-containing prostate epithelium. Production of ACT in the normal PSA-producing prostate epithelium was demonstrated by... (More)
Prostate-specific antigen (PSA) is a chymotrypsin-like serine protease exclusively produced by the prostate epithelium, and abundant in seminal fluid. In serum, PSA is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. Since the molecular basis for this is unclear, we have investigated whether or not ACT may be produced in the prostate gland. Immunocytochemistry, using either monoclonal or polyclonal IgGs, demonstrated specific immunostaining for ACT in normal PSA-containing prostate epithelium. Production of ACT in the normal PSA-producing prostate epithelium was demonstrated by means of nonradioactive in situ hybridization using 30-mer anti-sense DNA probes for ACT and for PSA. The ACT and PSA coding transcripts, as detected by in situ hybridization, were distributed perinuclearly, in contrast to the specific immunostaining for ACT and PSA which was most intense in the apical portion of the secretory cells. The results strongly suggest local production and release of ACT by the normal prostate epithelium that may be important for interaction between PSA and ACT in extracellular compartments. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Urology
volume
42
issue
5
pages
502 - 510
publisher
Elsevier
external identifiers
  • pmid:7694414
  • scopus:0027449422
ISSN
1527-9995
DOI
10.1016/0090-4295(93)90259-D
language
English
LU publication?
yes
id
27747594-b8a3-4c83-8dc6-a064704ed801 (old id 1107099)
date added to LUP
2008-07-30 11:24:19
date last changed
2017-01-29 03:37:55
@article{27747594-b8a3-4c83-8dc6-a064704ed801,
  abstract     = {Prostate-specific antigen (PSA) is a chymotrypsin-like serine protease exclusively produced by the prostate epithelium, and abundant in seminal fluid. In serum, PSA is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. Since the molecular basis for this is unclear, we have investigated whether or not ACT may be produced in the prostate gland. Immunocytochemistry, using either monoclonal or polyclonal IgGs, demonstrated specific immunostaining for ACT in normal PSA-containing prostate epithelium. Production of ACT in the normal PSA-producing prostate epithelium was demonstrated by means of nonradioactive in situ hybridization using 30-mer anti-sense DNA probes for ACT and for PSA. The ACT and PSA coding transcripts, as detected by in situ hybridization, were distributed perinuclearly, in contrast to the specific immunostaining for ACT and PSA which was most intense in the apical portion of the secretory cells. The results strongly suggest local production and release of ACT by the normal prostate epithelium that may be important for interaction between PSA and ACT in extracellular compartments.},
  author       = {Bjartell, Anders and Björk, Thomas and Matikainen, Maria-Terttu and Abrahamsson, Per-Anders and di Sant'Agnese, Anthony and Lilja, Hans},
  issn         = {1527-9995},
  language     = {eng},
  number       = {5},
  pages        = {502--510},
  publisher    = {Elsevier},
  series       = {Urology},
  title        = {Production of alpha-1-antichymotrypsin by PSA-containing cells of human prostate epithelium},
  url          = {http://dx.doi.org/10.1016/0090-4295(93)90259-D},
  volume       = {42},
  year         = {1993},
}