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Localization of hormone-sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes

Stenson, Lena LU ; Hoffmann, Anna Maria; Mulder, Hindrik LU ; Sundler, Frank LU ; Holm, Cecilia LU ; Bergh, Anders and Fredrikson, Gudrun (1994) In FEBS Letters 355(2). p.125-130
Abstract
Using in situ hybridization, hormone-sensitive lipase was found to be expressed in a stage-dependent manner in Sertoli cells of rat testis. No expression was found in Leydig cells but expression in spermatids could not be excluded. These results suggest a role for hormone-sensitive lipase in the metabolism of lipid droplets in Sertoli cells, in contrast to its previously proposed function in steroid biosynthesis. The expression of testicular hormone-sensitive lipase mRNA and protein, both larger in size compared to other tissues, coincided with the onset of spermatogenesis and was dependent on scrotal localization of the testis, suggesting a temperature-dependent, pretranslational regulation of expression.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hormone sensitive lipase, Cholesterol ester hydrolase, Testis, Sertoli cell, In situ hybridization
in
FEBS Letters
volume
355
issue
2
pages
125 - 130
publisher
Wiley-Blackwell
external identifiers
  • pmid:7982484
  • scopus:0028073597
ISSN
1873-3468
DOI
10.1016/0014-5793(94)01185-0
language
English
LU publication?
yes
id
68cf939c-dfa8-473a-b921-b8d9cd082102 (old id 1107741)
date added to LUP
2008-07-22 14:35:40
date last changed
2017-01-01 06:39:07
@article{68cf939c-dfa8-473a-b921-b8d9cd082102,
  abstract     = {Using in situ hybridization, hormone-sensitive lipase was found to be expressed in a stage-dependent manner in Sertoli cells of rat testis. No expression was found in Leydig cells but expression in spermatids could not be excluded. These results suggest a role for hormone-sensitive lipase in the metabolism of lipid droplets in Sertoli cells, in contrast to its previously proposed function in steroid biosynthesis. The expression of testicular hormone-sensitive lipase mRNA and protein, both larger in size compared to other tissues, coincided with the onset of spermatogenesis and was dependent on scrotal localization of the testis, suggesting a temperature-dependent, pretranslational regulation of expression.},
  author       = {Stenson, Lena and Hoffmann, Anna Maria and Mulder, Hindrik and Sundler, Frank and Holm, Cecilia and Bergh, Anders and Fredrikson, Gudrun},
  issn         = {1873-3468},
  keyword      = {Hormone sensitive lipase,Cholesterol ester hydrolase,Testis,Sertoli cell,In situ hybridization},
  language     = {eng},
  number       = {2},
  pages        = {125--130},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Localization of hormone-sensitive lipase to rat Sertoli cells and its expression in developing and degenerating testes},
  url          = {http://dx.doi.org/10.1016/0014-5793(94)01185-0},
  volume       = {355},
  year         = {1994},
}