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Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction

Adner, Mikael LU ; Erlinge, David LU ; Salford, Leif LU ; Yee, Frances; Wahlestedt, Claes and Edvinsson, Lars LU (1994) In European Journal of Pharmacology 261(3). p.281-284
Abstract
Antisense oligodeoxynucleotides to endothelin ETA receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ETB receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ETA receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA2 = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ETA receptors... (More)
Antisense oligodeoxynucleotides to endothelin ETA receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ETB receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ETA receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA2 = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ETA receptors mediating contraction in the human superficial temporal artery. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antisense oligodeoxynucleotide, Endothelin ETA receptor, Temporal artery, human
in
European Journal of Pharmacology
volume
261
issue
3
pages
281 - 284
publisher
Elsevier
external identifiers
  • pmid:7813549
  • scopus:0028169863
ISSN
1879-0712
DOI
10.1016/0014-2999(94)90118-X
language
English
LU publication?
yes
id
4ac00774-2f85-4776-9b6d-b14fe0384556 (old id 1108005)
date added to LUP
2008-07-23 11:13:25
date last changed
2017-01-01 04:37:41
@article{4ac00774-2f85-4776-9b6d-b14fe0384556,
  abstract     = {Antisense oligodeoxynucleotides to endothelin ETA receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ETB receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ETA receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA2 = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ETA receptors mediating contraction in the human superficial temporal artery.},
  author       = {Adner, Mikael and Erlinge, David and Salford, Leif and Yee, Frances and Wahlestedt, Claes and Edvinsson, Lars},
  issn         = {1879-0712},
  keyword      = {Antisense oligodeoxynucleotide,Endothelin ETA receptor,Temporal artery,human},
  language     = {eng},
  number       = {3},
  pages        = {281--284},
  publisher    = {Elsevier},
  series       = {European Journal of Pharmacology},
  title        = {Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction},
  url          = {http://dx.doi.org/10.1016/0014-2999(94)90118-X},
  volume       = {261},
  year         = {1994},
}