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Methodological aspects of flow cytometry DNA analysis in endometrial carcinoma, with special reference to sampling and reproducibility

Gudmundsson, Thorkell E.; Långström-Einarsson, Eva LU ; Alm, Per LU ; Anderson, Harald LU ; Baldetorp, Bo LU ; Fernö, Mårten LU ; Högberg, Thomas LU and Killander, Dick LU (1996) In Acta Oncologica 35(8). p.999-1006
Abstract
Two adjacent paraffin-embedded sections manifested concordance in ploidy status in 96% of cases (45/47), and the standard deviation (SD) for SPF was 2.7%. Analysis of 'micro-heterogeneity', within a distance of < or = 700 microm, yielded results for concordant ploidy status in 94% of cases, and the SD for SPF was 1.9% (n = 17). Frozen and paraffin-embedded material yielded concordant results for ploidy status in 87% (39/45) of cases, and SPF values were significantly lower (mean difference 1.5%) in the frozen samples. Diagnostic and repeat curettage material yielded concordant results for DNA ploidy status in 85% (40/47) of cases, and no significant difference in mean SPF (12% vs. 11%) was found. Discordant DNA ploidy results were... (More)
Two adjacent paraffin-embedded sections manifested concordance in ploidy status in 96% of cases (45/47), and the standard deviation (SD) for SPF was 2.7%. Analysis of 'micro-heterogeneity', within a distance of < or = 700 microm, yielded results for concordant ploidy status in 94% of cases, and the SD for SPF was 1.9% (n = 17). Frozen and paraffin-embedded material yielded concordant results for ploidy status in 87% (39/45) of cases, and SPF values were significantly lower (mean difference 1.5%) in the frozen samples. Diagnostic and repeat curettage material yielded concordant results for DNA ploidy status in 85% (40/47) of cases, and no significant difference in mean SPF (12% vs. 11%) was found. Discordant DNA ploidy results were attributable to small differences in the DNA histograms influencing the interpretation of near-diploid, near-tetraploid and small non-diploid cell populations, and the influence of debris on SPF estimation. On the basis of our findings and the practical advantage we recommend paraffin-embedded material from diagnostic curettage for FCM DNA analysis; the results are available sooner and the handling and transportation of tumor samples is more convenient. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Oncologica
volume
35
issue
8
pages
999 - 1006
publisher
Taylor & Francis
external identifiers
  • pmid:9023385
  • scopus:0030483322
ISSN
1651-226X
DOI
10.3109/02841869609100718
language
English
LU publication?
yes
id
ced7dc0a-896f-4f20-a57e-85c0682875c4 (old id 1110094)
date added to LUP
2008-07-23 10:45:39
date last changed
2017-01-01 07:00:38
@article{ced7dc0a-896f-4f20-a57e-85c0682875c4,
  abstract     = {Two adjacent paraffin-embedded sections manifested concordance in ploidy status in 96% of cases (45/47), and the standard deviation (SD) for SPF was 2.7%. Analysis of 'micro-heterogeneity', within a distance of &lt; or = 700 microm, yielded results for concordant ploidy status in 94% of cases, and the SD for SPF was 1.9% (n = 17). Frozen and paraffin-embedded material yielded concordant results for ploidy status in 87% (39/45) of cases, and SPF values were significantly lower (mean difference 1.5%) in the frozen samples. Diagnostic and repeat curettage material yielded concordant results for DNA ploidy status in 85% (40/47) of cases, and no significant difference in mean SPF (12% vs. 11%) was found. Discordant DNA ploidy results were attributable to small differences in the DNA histograms influencing the interpretation of near-diploid, near-tetraploid and small non-diploid cell populations, and the influence of debris on SPF estimation. On the basis of our findings and the practical advantage we recommend paraffin-embedded material from diagnostic curettage for FCM DNA analysis; the results are available sooner and the handling and transportation of tumor samples is more convenient.},
  author       = {Gudmundsson, Thorkell E. and Långström-Einarsson, Eva and Alm, Per and Anderson, Harald and Baldetorp, Bo and Fernö, Mårten and Högberg, Thomas and Killander, Dick},
  issn         = {1651-226X},
  language     = {eng},
  number       = {8},
  pages        = {999--1006},
  publisher    = {Taylor & Francis},
  series       = {Acta Oncologica},
  title        = {Methodological aspects of flow cytometry DNA analysis in endometrial carcinoma, with special reference to sampling and reproducibility},
  url          = {http://dx.doi.org/10.3109/02841869609100718},
  volume       = {35},
  year         = {1996},
}