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Determination of hexahydrophthalic and methylhexahydrophthalic acids in urine by gas chromatography-negative-ion chemical-ionisation mass spectrometry

Jönsson, Bo A LU and Lindh, Christian LU (1996) In Chromatographia 42(11-12). p.647-652
Abstract
A method for determining hexahydrophthalic (HHP acid) and methylhexahydrophthalic acids (MHHP acid) from human urine was developed. These acids are metabolites of the highly sensitising hexahydrophthalic anhydride and methylhexahydrophthalic anhydride. The acids were purified from urine by liquid-solid extraction and derivatised with pentafluorobenzyl bromide to the corresponding esters. These were analysed by GC-MS in the negative-ion chemical-ionisation mode with ammonia moderating gas. Deuterium labelled HHP acid and MHHP acid were internal standards. The precision for HHP acid was 3–6% in the range 70–620 ng mL–1 and that for MHHP acid was 2–8% in the range 60–680 ng mL–1. Overall recovery of HHP acid from urine was 74–88% and that for... (More)
A method for determining hexahydrophthalic (HHP acid) and methylhexahydrophthalic acids (MHHP acid) from human urine was developed. These acids are metabolites of the highly sensitising hexahydrophthalic anhydride and methylhexahydrophthalic anhydride. The acids were purified from urine by liquid-solid extraction and derivatised with pentafluorobenzyl bromide to the corresponding esters. These were analysed by GC-MS in the negative-ion chemical-ionisation mode with ammonia moderating gas. Deuterium labelled HHP acid and MHHP acid were internal standards. The precision for HHP acid was 3–6% in the range 70–620 ng mL–1 and that for MHHP acid was 2–8% in the range 60–680 ng mL–1. Overall recovery of HHP acid from urine was 74–88% and that for MHHP acid 98–101%. Detection limits were 11 ng HHP acid mL–1 urine and 17 ng MHHP acid mL–1 urine. There were no significant differences between determinations of HHP acid by a previous method and the present method. Some instability of the acids in urine were found after extended storage at –20°C. The method was applicable for determination of HHP acid and MHHP acid from exposed workers. (Less)
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author
organization
publishing date
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Contribution to journal
publication status
published
subject
keywords
Industrial hygiene, Biological monitoring, Pentafluorobenzyl derivatives, Hexahydrophthalic and methylhexahydrophthalic acid, Gas chromatography-mass spectrometry
in
Chromatographia
volume
42
issue
11-12
pages
647 - 652
publisher
Vieweg Verlag
external identifiers
  • scopus:0029680507
ISSN
0009-5893
DOI
10.1007/BF02267696
language
English
LU publication?
yes
id
ba0fe718-720f-4611-b81a-df25dcf4a247 (old id 1110158)
date added to LUP
2008-07-29 08:16:29
date last changed
2017-01-01 06:55:36
@article{ba0fe718-720f-4611-b81a-df25dcf4a247,
  abstract     = {A method for determining hexahydrophthalic (HHP acid) and methylhexahydrophthalic acids (MHHP acid) from human urine was developed. These acids are metabolites of the highly sensitising hexahydrophthalic anhydride and methylhexahydrophthalic anhydride. The acids were purified from urine by liquid-solid extraction and derivatised with pentafluorobenzyl bromide to the corresponding esters. These were analysed by GC-MS in the negative-ion chemical-ionisation mode with ammonia moderating gas. Deuterium labelled HHP acid and MHHP acid were internal standards. The precision for HHP acid was 3–6% in the range 70–620 ng mL–1 and that for MHHP acid was 2–8% in the range 60–680 ng mL–1. Overall recovery of HHP acid from urine was 74–88% and that for MHHP acid 98–101%. Detection limits were 11 ng HHP acid mL–1 urine and 17 ng MHHP acid mL–1 urine. There were no significant differences between determinations of HHP acid by a previous method and the present method. Some instability of the acids in urine were found after extended storage at –20°C. The method was applicable for determination of HHP acid and MHHP acid from exposed workers.},
  author       = {Jönsson, Bo A and Lindh, Christian},
  issn         = {0009-5893},
  keyword      = {Industrial hygiene,Biological monitoring,Pentafluorobenzyl derivatives,Hexahydrophthalic and methylhexahydrophthalic acid,Gas chromatography-mass spectrometry},
  language     = {eng},
  number       = {11-12},
  pages        = {647--652},
  publisher    = {Vieweg Verlag},
  series       = {Chromatographia},
  title        = {Determination of hexahydrophthalic and methylhexahydrophthalic acids in urine by gas chromatography-negative-ion chemical-ionisation mass spectrometry},
  url          = {http://dx.doi.org/10.1007/BF02267696},
  volume       = {42},
  year         = {1996},
}