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Analysis of methylhexahydrophthalic acid in human urine

Lindh, Christian LU and Jönsson, Bo A LU (1996) In Chromatographia 43(11-12). p.668-670
Abstract
A method for analysis of urinary methylhexahydrophthalic acid (MHHP acid), a metabolite of the highly sensitising methylhexahydrophthalic anhydride, is described. The method is based on a double liquid-solid extraction of the MHHP acid from urine, esterification with methanol and boron trifluoride catalysis, and analysis with gas chromatography-mass spectrometry in the electron impact mode. The detection limit was 3 ng mL–1 and the precision between 2 and 5% in the range 34 to 340 ng mL–1. The method gave similar results compared to another method. Urine stored for five years at –20°C lost between 38 and 86% of the MHHP acid (range 16–85 ng mL–1).
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Industrial hygiene, Biological monitoring, Methylhexahydrophthalic acid, Gas chromatography-mass spectrometry
in
Chromatographia
volume
43
issue
11-12
pages
668 - 670
publisher
Vieweg Verlag
external identifiers
  • scopus:0029966795
ISSN
0009-5893
DOI
10.1007/BF02292986
language
English
LU publication?
yes
id
817e1f19-b785-4208-96eb-a600a2e4b457 (old id 1110481)
date added to LUP
2008-07-29 08:20:17
date last changed
2017-01-01 06:49:06
@article{817e1f19-b785-4208-96eb-a600a2e4b457,
  abstract     = {A method for analysis of urinary methylhexahydrophthalic acid (MHHP acid), a metabolite of the highly sensitising methylhexahydrophthalic anhydride, is described. The method is based on a double liquid-solid extraction of the MHHP acid from urine, esterification with methanol and boron trifluoride catalysis, and analysis with gas chromatography-mass spectrometry in the electron impact mode. The detection limit was 3 ng mL–1 and the precision between 2 and 5% in the range 34 to 340 ng mL–1. The method gave similar results compared to another method. Urine stored for five years at –20°C lost between 38 and 86% of the MHHP acid (range 16–85 ng mL–1).},
  author       = {Lindh, Christian and Jönsson, Bo A},
  issn         = {0009-5893},
  keyword      = {Industrial hygiene,Biological monitoring,Methylhexahydrophthalic acid,Gas chromatography-mass spectrometry},
  language     = {eng},
  number       = {11-12},
  pages        = {668--670},
  publisher    = {Vieweg Verlag},
  series       = {Chromatographia},
  title        = {Analysis of methylhexahydrophthalic acid in human urine},
  url          = {http://dx.doi.org/10.1007/BF02292986},
  volume       = {43},
  year         = {1996},
}