Analysis of methylhexahydrophthalic acid in human urine
(1996) In Chromatographia 43(11-12). p.668-670- Abstract
- A method for analysis of urinary methylhexahydrophthalic acid (MHHP acid), a metabolite of the highly sensitising methylhexahydrophthalic anhydride, is described. The method is based on a double liquid-solid extraction of the MHHP acid from urine, esterification with methanol and boron trifluoride catalysis, and analysis with gas chromatography-mass spectrometry in the electron impact mode. The detection limit was 3 ng mL–1 and the precision between 2 and 5% in the range 34 to 340 ng mL–1. The method gave similar results compared to another method. Urine stored for five years at –20°C lost between 38 and 86% of the MHHP acid (range 16–85 ng mL–1).
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1110481
- author
- Lindh, Christian LU and Jönsson, Bo A LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Industrial hygiene, Biological monitoring, Methylhexahydrophthalic acid, Gas chromatography-mass spectrometry
- in
- Chromatographia
- volume
- 43
- issue
- 11-12
- pages
- 668 - 670
- publisher
- Vieweg Verlag
- external identifiers
-
- scopus:0029966795
- ISSN
- 0009-5893
- DOI
- 10.1007/BF02292986
- language
- English
- LU publication?
- yes
- id
- 817e1f19-b785-4208-96eb-a600a2e4b457 (old id 1110481)
- date added to LUP
- 2016-04-01 15:52:13
- date last changed
- 2022-01-28 07:38:49
@article{817e1f19-b785-4208-96eb-a600a2e4b457, abstract = {{A method for analysis of urinary methylhexahydrophthalic acid (MHHP acid), a metabolite of the highly sensitising methylhexahydrophthalic anhydride, is described. The method is based on a double liquid-solid extraction of the MHHP acid from urine, esterification with methanol and boron trifluoride catalysis, and analysis with gas chromatography-mass spectrometry in the electron impact mode. The detection limit was 3 ng mL–1 and the precision between 2 and 5% in the range 34 to 340 ng mL–1. The method gave similar results compared to another method. Urine stored for five years at –20°C lost between 38 and 86% of the MHHP acid (range 16–85 ng mL–1).}}, author = {{Lindh, Christian and Jönsson, Bo A}}, issn = {{0009-5893}}, keywords = {{Industrial hygiene; Biological monitoring; Methylhexahydrophthalic acid; Gas chromatography-mass spectrometry}}, language = {{eng}}, number = {{11-12}}, pages = {{668--670}}, publisher = {{Vieweg Verlag}}, series = {{Chromatographia}}, title = {{Analysis of methylhexahydrophthalic acid in human urine}}, url = {{http://dx.doi.org/10.1007/BF02292986}}, doi = {{10.1007/BF02292986}}, volume = {{43}}, year = {{1996}}, }