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Inflammatory reaction dependence on implant localization in rat soft tissue models

Rosengren, Agneta LU ; Danielsen, Nils LU and Bjursten, Lars Magnus LU (1997) In Biomaterials 18(14). p.979-987
Abstract
This study compares two different implantation models in soft tissue in rat abdominal wall with regard to inflammatory reactions. Titanium rods and discs, penetrating or not penetrating the peritoneal wall respectively, were implanted. After 3, 10 or 30 days the distribution of monocytes/macrophages and cytokines (interleukin-1 and transforming growth factor-beta) in the tissue adjacent to the implants was investigated under immunohistochemistry. The macrophage-specific antibody, ED1, was used for the identification of newly recruited macrophages and the ED2 antibody was used for the mature tissue macrophages. After 10 days the non-penetrating implants had a larger number of cells close to the implant than the penetrating implants. The... (More)
This study compares two different implantation models in soft tissue in rat abdominal wall with regard to inflammatory reactions. Titanium rods and discs, penetrating or not penetrating the peritoneal wall respectively, were implanted. After 3, 10 or 30 days the distribution of monocytes/macrophages and cytokines (interleukin-1 and transforming growth factor-beta) in the tissue adjacent to the implants was investigated under immunohistochemistry. The macrophage-specific antibody, ED1, was used for the identification of newly recruited macrophages and the ED2 antibody was used for the mature tissue macrophages. After 10 days the non-penetrating implants had a larger number of cells close to the implant than the penetrating implants. The opposite was seen after 30 days implantation, with a larger number of cells around the penetrating implants. At all time intervals the penetrating implants had a thicker reactive capsule. The cytokines interleukin-1beta and transforming growth factor-beta could be detected in the reactive tissue adjacent to both types of implants, without obvious differences for the two implant situations. The biocompatibility of a material appears to be influenced by the localization of the implant. In addition, it seems to be of importance to extend the follow-up periods further, as we cannot assume that steady state is reached at 30 days implantation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Titanium, peritoneal implants, macrophages, immunohistochemistry, soft tissue
in
Biomaterials
volume
18
issue
14
pages
979 - 987
publisher
Elsevier
external identifiers
  • pmid:9212193
  • scopus:0031193361
ISSN
1878-5905
DOI
10.1016/S0142-9612(97)00012-4
language
English
LU publication?
yes
id
13d73a26-1b94-4dd4-bc09-b69d1842b42e (old id 1111334)
date added to LUP
2008-07-17 11:29:57
date last changed
2017-05-28 03:29:11
@article{13d73a26-1b94-4dd4-bc09-b69d1842b42e,
  abstract     = {This study compares two different implantation models in soft tissue in rat abdominal wall with regard to inflammatory reactions. Titanium rods and discs, penetrating or not penetrating the peritoneal wall respectively, were implanted. After 3, 10 or 30 days the distribution of monocytes/macrophages and cytokines (interleukin-1 and transforming growth factor-beta) in the tissue adjacent to the implants was investigated under immunohistochemistry. The macrophage-specific antibody, ED1, was used for the identification of newly recruited macrophages and the ED2 antibody was used for the mature tissue macrophages. After 10 days the non-penetrating implants had a larger number of cells close to the implant than the penetrating implants. The opposite was seen after 30 days implantation, with a larger number of cells around the penetrating implants. At all time intervals the penetrating implants had a thicker reactive capsule. The cytokines interleukin-1beta and transforming growth factor-beta could be detected in the reactive tissue adjacent to both types of implants, without obvious differences for the two implant situations. The biocompatibility of a material appears to be influenced by the localization of the implant. In addition, it seems to be of importance to extend the follow-up periods further, as we cannot assume that steady state is reached at 30 days implantation.},
  author       = {Rosengren, Agneta and Danielsen, Nils and Bjursten, Lars Magnus},
  issn         = {1878-5905},
  keyword      = {Titanium,peritoneal implants,macrophages,immunohistochemistry,soft tissue},
  language     = {eng},
  number       = {14},
  pages        = {979--987},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Inflammatory reaction dependence on implant localization in rat soft tissue models},
  url          = {http://dx.doi.org/10.1016/S0142-9612(97)00012-4},
  volume       = {18},
  year         = {1997},
}