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Determination of N-methylsuccinimide and 2-hydroxy-N-methylsuccinimide in human urine and plasma

Jönsson, Bo A LU and Åkesson, Bengt LU (1997) In Journal of Chromatography. B, Biomedical Sciences and Applications 704(1-2). p.151-158
Abstract
A method for determination of N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine and of MSI in human plasma was developed. MSI and 2-HMSI are metabolites of the widely used organic solvent N-methyl-2-pyrrolidone (NMP). MSI and 2-HMSI were purified from urine and plasma by C8 solid-phase extraction and analysed by gas chromatography-mass spectrometry in the negative-ion chemical ionisation mode. The intra-day precisions in urine were 2-6% for MSI (50 and 400 ng/ml) and 3-5% for 2-HMSI (1000 and 8000 ng/ml). For MSI in plasma it was 2% (60 and 1200 ng/ml). The between-day precisions in urine were 3-4% for MSI (100 and 1000 ng/ml) and 2-4% for 2-HMSI (10,000 and 18,000 ng/ml) and 3-4% for MSI in plasma (100... (More)
A method for determination of N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine and of MSI in human plasma was developed. MSI and 2-HMSI are metabolites of the widely used organic solvent N-methyl-2-pyrrolidone (NMP). MSI and 2-HMSI were purified from urine and plasma by C8 solid-phase extraction and analysed by gas chromatography-mass spectrometry in the negative-ion chemical ionisation mode. The intra-day precisions in urine were 2-6% for MSI (50 and 400 ng/ml) and 3-5% for 2-HMSI (1000 and 8000 ng/ml). For MSI in plasma it was 2% (60 and 1200 ng/ml). The between-day precisions in urine were 3-4% for MSI (100 and 1000 ng/ml) and 2-4% for 2-HMSI (10,000 and 18,000 ng/ml) and 3-4% for MSI in plasma (100 and 900 ng/ml). The recoveries from urine were 109-117% for MSI (50 and 400 ng/ml) and 81-89% for 2-HMSI (1000 and 8000 ng/ml). The recovery of MSI from plasma was 91-101% (50 and 500 ng/ml). The detection limits for MSI were 3 ng/ml in urine and 1 ng/ml in plasma and that of 2-HMSI in urine was 200 ng/ml. The method is applicable for analysis of urine and plasma samples from workers exposed to NMP. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
N-Methylsuccinimide, 2-Hydroxy-N-methylsuccinimide, N-Methyl-2-pyrrolidone
in
Journal of Chromatography. B, Biomedical Sciences and Applications
volume
704
issue
1-2
pages
151 - 158
publisher
Elsevier
external identifiers
  • pmid:9518144
  • scopus:2542508150
ISSN
1387-2273
DOI
10.1016/S0378-4347(97)00452-0
language
English
LU publication?
yes
id
c5dfd62a-6b6a-4b0c-98e7-4dea203dddd4 (old id 1111493)
date added to LUP
2008-07-17 14:20:46
date last changed
2017-01-01 07:13:51
@article{c5dfd62a-6b6a-4b0c-98e7-4dea203dddd4,
  abstract     = {A method for determination of N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine and of MSI in human plasma was developed. MSI and 2-HMSI are metabolites of the widely used organic solvent N-methyl-2-pyrrolidone (NMP). MSI and 2-HMSI were purified from urine and plasma by C8 solid-phase extraction and analysed by gas chromatography-mass spectrometry in the negative-ion chemical ionisation mode. The intra-day precisions in urine were 2-6% for MSI (50 and 400 ng/ml) and 3-5% for 2-HMSI (1000 and 8000 ng/ml). For MSI in plasma it was 2% (60 and 1200 ng/ml). The between-day precisions in urine were 3-4% for MSI (100 and 1000 ng/ml) and 2-4% for 2-HMSI (10,000 and 18,000 ng/ml) and 3-4% for MSI in plasma (100 and 900 ng/ml). The recoveries from urine were 109-117% for MSI (50 and 400 ng/ml) and 81-89% for 2-HMSI (1000 and 8000 ng/ml). The recovery of MSI from plasma was 91-101% (50 and 500 ng/ml). The detection limits for MSI were 3 ng/ml in urine and 1 ng/ml in plasma and that of 2-HMSI in urine was 200 ng/ml. The method is applicable for analysis of urine and plasma samples from workers exposed to NMP.},
  author       = {Jönsson, Bo A and Åkesson, Bengt},
  issn         = {1387-2273},
  keyword      = {N-Methylsuccinimide,2-Hydroxy-N-methylsuccinimide,N-Methyl-2-pyrrolidone},
  language     = {eng},
  number       = {1-2},
  pages        = {151--158},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B, Biomedical Sciences and Applications},
  title        = {Determination of N-methylsuccinimide and 2-hydroxy-N-methylsuccinimide in human urine and plasma},
  url          = {http://dx.doi.org/10.1016/S0378-4347(97)00452-0},
  volume       = {704},
  year         = {1997},
}