Advanced

Determination of hexahydrophthalic acid and methylhexahydrophthalic acid in plasma after derivatisation with pentafluorobenzyl bromide using gas chromatography and mass spectrometric detection

Lindh, Christian LU and Jönsson, Bo A LU (1997) In Journal of Chromatography. B, Biomedical Sciences and Applications 691(2). p.331-339
Abstract
A method for the simultaneous determination of hexahydrophthalic acid (HHP acid) and methylhexahydrophthalic acid (MHHP acid) in human plasma was developed. The procedure was a rapid, single step extractive derivatisation with pentafluorobenzyl bromide as the derivatisation agent. The formed pentafluorobenzyl esters were analysed by gas chromatography-mass spectrometry in negative ion chemical ionisation mode with ammonia as the moderating gas. Deuterium-labeled HHP acid and MHHP acid were used as internal standards. The detection limit was 0.4 ng/ml for HHP acid (m/z 153) and 0.3 ng/ml for MHHP acid (m/z 365). The within-day precision of the method was between 2 and 3% and the between-day precision was between 3 and 12%. The overall... (More)
A method for the simultaneous determination of hexahydrophthalic acid (HHP acid) and methylhexahydrophthalic acid (MHHP acid) in human plasma was developed. The procedure was a rapid, single step extractive derivatisation with pentafluorobenzyl bromide as the derivatisation agent. The formed pentafluorobenzyl esters were analysed by gas chromatography-mass spectrometry in negative ion chemical ionisation mode with ammonia as the moderating gas. Deuterium-labeled HHP acid and MHHP acid were used as internal standards. The detection limit was 0.4 ng/ml for HHP acid (m/z 153) and 0.3 ng/ml for MHHP acid (m/z 365). The within-day precision of the method was between 2 and 3% and the between-day precision was between 3 and 12%. The overall recovery was between 65 and 83%. A comparison between HHP acid determinations with a previous and this method showed that the methods gave similar results. The method was applicable for analysis of plasma from occupationally exposed workers. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hexahydrophthalic acid, Methylhexahydrophthalic acid
in
Journal of Chromatography. B, Biomedical Sciences and Applications
volume
691
issue
2
pages
331 - 339
publisher
Elsevier
external identifiers
  • pmid:9174269
  • scopus:0343118748
ISSN
1387-2273
DOI
10.1016/S0378-4347(96)00479-3
language
English
LU publication?
yes
id
fcaeae8a-4053-48e9-b9c8-2eff5415593c (old id 1111497)
date added to LUP
2008-07-17 14:24:51
date last changed
2017-08-27 05:32:03
@article{fcaeae8a-4053-48e9-b9c8-2eff5415593c,
  abstract     = {A method for the simultaneous determination of hexahydrophthalic acid (HHP acid) and methylhexahydrophthalic acid (MHHP acid) in human plasma was developed. The procedure was a rapid, single step extractive derivatisation with pentafluorobenzyl bromide as the derivatisation agent. The formed pentafluorobenzyl esters were analysed by gas chromatography-mass spectrometry in negative ion chemical ionisation mode with ammonia as the moderating gas. Deuterium-labeled HHP acid and MHHP acid were used as internal standards. The detection limit was 0.4 ng/ml for HHP acid (m/z 153) and 0.3 ng/ml for MHHP acid (m/z 365). The within-day precision of the method was between 2 and 3% and the between-day precision was between 3 and 12%. The overall recovery was between 65 and 83%. A comparison between HHP acid determinations with a previous and this method showed that the methods gave similar results. The method was applicable for analysis of plasma from occupationally exposed workers.},
  author       = {Lindh, Christian and Jönsson, Bo A},
  issn         = {1387-2273},
  keyword      = {Hexahydrophthalic acid,Methylhexahydrophthalic acid},
  language     = {eng},
  number       = {2},
  pages        = {331--339},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B, Biomedical Sciences and Applications},
  title        = {Determination of hexahydrophthalic acid and methylhexahydrophthalic acid in plasma after derivatisation with pentafluorobenzyl bromide using gas chromatography and mass spectrometric detection},
  url          = {http://dx.doi.org/10.1016/S0378-4347(96)00479-3},
  volume       = {691},
  year         = {1997},
}