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Epidermal growth factor induces cell proliferation in mouse pancreas and salivary glands

Ohlsson, Bodil LU ; Hjalmarsson, Claes LU ; Ihse, Ingemar LU and Axelson, Jan LU (1997) In Pancreas 14(1). p.94-98
Abstract
Epidermal growth factor (EGF) is a mitogenic stimulus in many tissues and occurs in large amounts in the pancreas and salivary glands. Whether EGF is mitogenic in the pancreas is controversial, and the EGF effect has not been studied in the salivary glands. Therefore, the aim of the present study was to investigate the possible effects on the pancreas and parotid and submandibular glands at different time intervals after exogenous EGF administration. Human recombinant EGF was infused subcutaneously by osmotic minipumps in three groups of mice (for 1, 3, and 7 days, respectively) at a dosage of 10 micrograms/kg/h (1.6 mumol/kg/h). Tritiated thymidine was infused intraperitoneally by osmotic minipumps for the same time periods, but only for... (More)
Epidermal growth factor (EGF) is a mitogenic stimulus in many tissues and occurs in large amounts in the pancreas and salivary glands. Whether EGF is mitogenic in the pancreas is controversial, and the EGF effect has not been studied in the salivary glands. Therefore, the aim of the present study was to investigate the possible effects on the pancreas and parotid and submandibular glands at different time intervals after exogenous EGF administration. Human recombinant EGF was infused subcutaneously by osmotic minipumps in three groups of mice (for 1, 3, and 7 days, respectively) at a dosage of 10 micrograms/kg/h (1.6 mumol/kg/h). Tritiated thymidine was infused intraperitoneally by osmotic minipumps for the same time periods, but only for the last 3 days in the 7-day group. After 1 day the pancreas increased in weight and the increase persisted throughout the study. No effect was seen on the parotid or submandibular gland wet weight. A slight transient increase in pancreas protein content was observed, whereas amylase content was unaffected. The labeling index of serous and ductal cells in the parotid gland increased from the third day. After 7 days, all cell types studied in the pancreas and parotid and submandibular glands were in a hyperproliferative state. The results show that EGF evoked a strong proliferative response on all cell types studied in the pancreas and parotid and submandibular glands. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Pancreas
volume
14
issue
1
pages
94 - 98
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:8981513
  • scopus:0031035068
ISSN
0885-3177
language
English
LU publication?
yes
id
9f16e983-5671-4b61-b518-67fd4eb21b69 (old id 1111718)
date added to LUP
2008-07-18 11:18:53
date last changed
2017-01-22 03:29:27
@article{9f16e983-5671-4b61-b518-67fd4eb21b69,
  abstract     = {Epidermal growth factor (EGF) is a mitogenic stimulus in many tissues and occurs in large amounts in the pancreas and salivary glands. Whether EGF is mitogenic in the pancreas is controversial, and the EGF effect has not been studied in the salivary glands. Therefore, the aim of the present study was to investigate the possible effects on the pancreas and parotid and submandibular glands at different time intervals after exogenous EGF administration. Human recombinant EGF was infused subcutaneously by osmotic minipumps in three groups of mice (for 1, 3, and 7 days, respectively) at a dosage of 10 micrograms/kg/h (1.6 mumol/kg/h). Tritiated thymidine was infused intraperitoneally by osmotic minipumps for the same time periods, but only for the last 3 days in the 7-day group. After 1 day the pancreas increased in weight and the increase persisted throughout the study. No effect was seen on the parotid or submandibular gland wet weight. A slight transient increase in pancreas protein content was observed, whereas amylase content was unaffected. The labeling index of serous and ductal cells in the parotid gland increased from the third day. After 7 days, all cell types studied in the pancreas and parotid and submandibular glands were in a hyperproliferative state. The results show that EGF evoked a strong proliferative response on all cell types studied in the pancreas and parotid and submandibular glands.},
  author       = {Ohlsson, Bodil and Hjalmarsson, Claes and Ihse, Ingemar and Axelson, Jan},
  issn         = {0885-3177},
  language     = {eng},
  number       = {1},
  pages        = {94--98},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Pancreas},
  title        = {Epidermal growth factor induces cell proliferation in mouse pancreas and salivary glands},
  volume       = {14},
  year         = {1997},
}