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Quantification method of human hemoglobin adducts from hexahydrophthalic anhydride and methylhexahydrophthalic anhydride

Lindh, Christian LU and Jönsson, Bo A LU (1998) In Journal of Chromatography. B, Biomedical Sciences and Applications 710(1-2). p.81-90
Abstract
A method was developed for the determination of human hemoglobin (Hb) adducts from hexahydrophthalic anhydride (HHPA) and methylhexahydrophthalic anhydride (MHHPA). The procedure includes lysis of erythrocytes, dialysis of the Hb-solution followed by acid hydrolysis. The released hexahydrophthalic (HHP) acid and methylhexahydrophthalic (MHHP) acid were purified using a combined liquid-liquid and solid-phase extraction procedure followed by derivatization with pentafluorobenzyl bromide. The derivatives were analyzed using GC-MS in negative ion chemical ionization mode with ammonia as moderating gas. As internal standards, deuterium-labeled HHP and MHHP acids were used. The detection limits were 0.3 pmol/g Hb for HHP acid and 0.9 pmol/g Hb... (More)
A method was developed for the determination of human hemoglobin (Hb) adducts from hexahydrophthalic anhydride (HHPA) and methylhexahydrophthalic anhydride (MHHPA). The procedure includes lysis of erythrocytes, dialysis of the Hb-solution followed by acid hydrolysis. The released hexahydrophthalic (HHP) acid and methylhexahydrophthalic (MHHP) acid were purified using a combined liquid-liquid and solid-phase extraction procedure followed by derivatization with pentafluorobenzyl bromide. The derivatives were analyzed using GC-MS in negative ion chemical ionization mode with ammonia as moderating gas. As internal standards, deuterium-labeled HHP and MHHP acids were used. The detection limits were 0.3 pmol/g Hb for HHP acid and 0.9 pmol/g Hb for MHHP acid. The between-day precisions for HHP acid were 18% at 2 pmol/g Hb and 10% at 13 pmol/g Hb. For MHHP acid, the precision was 27% at 2 pmol/g Hb and 14% at 22 pmol/g Hb. The method was applicable for analysis of Hb adducts from workers occupationally exposed to HHPA and MHHPA. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hexahydrophthalic anhydride, Hemogloblin, Methylhexahydrophthalic anhydride
in
Journal of Chromatography. B, Biomedical Sciences and Applications
volume
710
issue
1-2
pages
81 - 90
publisher
Elsevier
external identifiers
  • pmid:9686874
  • scopus:0032511242
ISSN
1387-2273
DOI
10.1016/S0378-4347(98)00121-2
language
English
LU publication?
yes
id
f5179cef-8026-448b-b821-8dde7ee3f36e (old id 1113365)
date added to LUP
2008-07-14 16:55:40
date last changed
2017-05-07 04:20:01
@article{f5179cef-8026-448b-b821-8dde7ee3f36e,
  abstract     = {A method was developed for the determination of human hemoglobin (Hb) adducts from hexahydrophthalic anhydride (HHPA) and methylhexahydrophthalic anhydride (MHHPA). The procedure includes lysis of erythrocytes, dialysis of the Hb-solution followed by acid hydrolysis. The released hexahydrophthalic (HHP) acid and methylhexahydrophthalic (MHHP) acid were purified using a combined liquid-liquid and solid-phase extraction procedure followed by derivatization with pentafluorobenzyl bromide. The derivatives were analyzed using GC-MS in negative ion chemical ionization mode with ammonia as moderating gas. As internal standards, deuterium-labeled HHP and MHHP acids were used. The detection limits were 0.3 pmol/g Hb for HHP acid and 0.9 pmol/g Hb for MHHP acid. The between-day precisions for HHP acid were 18% at 2 pmol/g Hb and 10% at 13 pmol/g Hb. For MHHP acid, the precision was 27% at 2 pmol/g Hb and 14% at 22 pmol/g Hb. The method was applicable for analysis of Hb adducts from workers occupationally exposed to HHPA and MHHPA.},
  author       = {Lindh, Christian and Jönsson, Bo A},
  issn         = {1387-2273},
  keyword      = {Hexahydrophthalic anhydride,Hemogloblin,Methylhexahydrophthalic anhydride},
  language     = {eng},
  number       = {1-2},
  pages        = {81--90},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B, Biomedical Sciences and Applications},
  title        = {Quantification method of human hemoglobin adducts from hexahydrophthalic anhydride and methylhexahydrophthalic anhydride},
  url          = {http://dx.doi.org/10.1016/S0378-4347(98)00121-2},
  volume       = {710},
  year         = {1998},
}