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Human achaete-scute homologue 1 (HASH-1) is downregulated in differentiating neuroblastoma cells

Söderholm, H; Örtoft, Eva; Johansson, Irja; Ljungberg, June LU ; Larsson, Christer LU ; Axelson, Håkan LU and Påhlman, Sven LU (1999) In Biochemical and Biophysical Research Communications 256(3). p.557-563
Abstract
The mammalian achaete-scute homologue, MASH-1, is crucial for early development of the sympathetic nervous system and is transiently expressed in sympathetic neuroblasts during embryogenesis. Here we report that the human homologue (HASH-1) was expressed in all analyzed cell lines (6/6) derived from the sympathetic nervous system tumor neuroblastoma. The majority of small-cell lung carcinoma (4/5) cell lines tested expressed HASH-1, while other nonneuronal/non-neuroendocrine cell lines were negative. Induced differentiation of neuroblastoma cells resulted in HASH-1 downregulation. This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of... (More)
The mammalian achaete-scute homologue, MASH-1, is crucial for early development of the sympathetic nervous system and is transiently expressed in sympathetic neuroblasts during embryogenesis. Here we report that the human homologue (HASH-1) was expressed in all analyzed cell lines (6/6) derived from the sympathetic nervous system tumor neuroblastoma. The majority of small-cell lung carcinoma (4/5) cell lines tested expressed HASH-1, while other nonneuronal/non-neuroendocrine cell lines were negative. Induced differentiation of neuroblastoma cells resulted in HASH-1 downregulation. This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of exogenous HASH-1 did not alter the capacity of the neuroblastoma cells to differentiate in response to differentiation-inducing agents. It is concluded that moderate HASH-1 expression does not compromise the capacity of these cells to differentiate. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical and Biophysical Research Communications
volume
256
issue
3
pages
557 - 563
publisher
Elsevier
external identifiers
  • pmid:10080936
  • scopus:0342668639
ISSN
1090-2104
DOI
10.1006/bbrc.1999.0314
language
English
LU publication?
yes
id
f4f404ff-4c1a-41d8-a794-b5c5d4fc4ff2 (old id 1114121)
date added to LUP
2008-07-03 13:43:31
date last changed
2017-01-15 04:16:46
@article{f4f404ff-4c1a-41d8-a794-b5c5d4fc4ff2,
  abstract     = {The mammalian achaete-scute homologue, MASH-1, is crucial for early development of the sympathetic nervous system and is transiently expressed in sympathetic neuroblasts during embryogenesis. Here we report that the human homologue (HASH-1) was expressed in all analyzed cell lines (6/6) derived from the sympathetic nervous system tumor neuroblastoma. The majority of small-cell lung carcinoma (4/5) cell lines tested expressed HASH-1, while other nonneuronal/non-neuroendocrine cell lines were negative. Induced differentiation of neuroblastoma cells resulted in HASH-1 downregulation. This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of exogenous HASH-1 did not alter the capacity of the neuroblastoma cells to differentiate in response to differentiation-inducing agents. It is concluded that moderate HASH-1 expression does not compromise the capacity of these cells to differentiate.},
  author       = {Söderholm, H and Örtoft, Eva and Johansson, Irja and Ljungberg, June and Larsson, Christer and Axelson, Håkan and Påhlman, Sven},
  issn         = {1090-2104},
  language     = {eng},
  number       = {3},
  pages        = {557--563},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Human achaete-scute homologue 1 (HASH-1) is downregulated in differentiating neuroblastoma cells},
  url          = {http://dx.doi.org/10.1006/bbrc.1999.0314},
  volume       = {256},
  year         = {1999},
}