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Human achaete-scute homologue 1 (HASH-1) is downregulated in differentiating neuroblastoma cells

Söderholm, H ; Örtoft, Eva ; Johansson, Irja ; Ljungberg, June LU ; Larsson, Christer LU ; Axelson, Håkan LU and Påhlman, Sven LU (1999) In Biochemical and Biophysical Research Communications 256(3). p.557-563
Abstract
The mammalian achaete-scute homologue, MASH-1, is crucial for early development of the sympathetic nervous system and is transiently expressed in sympathetic neuroblasts during embryogenesis. Here we report that the human homologue (HASH-1) was expressed in all analyzed cell lines (6/6) derived from the sympathetic nervous system tumor neuroblastoma. The majority of small-cell lung carcinoma (4/5) cell lines tested expressed HASH-1, while other nonneuronal/non-neuroendocrine cell lines were negative. Induced differentiation of neuroblastoma cells resulted in HASH-1 downregulation. This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of... (More)
The mammalian achaete-scute homologue, MASH-1, is crucial for early development of the sympathetic nervous system and is transiently expressed in sympathetic neuroblasts during embryogenesis. Here we report that the human homologue (HASH-1) was expressed in all analyzed cell lines (6/6) derived from the sympathetic nervous system tumor neuroblastoma. The majority of small-cell lung carcinoma (4/5) cell lines tested expressed HASH-1, while other nonneuronal/non-neuroendocrine cell lines were negative. Induced differentiation of neuroblastoma cells resulted in HASH-1 downregulation. This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of exogenous HASH-1 did not alter the capacity of the neuroblastoma cells to differentiate in response to differentiation-inducing agents. It is concluded that moderate HASH-1 expression does not compromise the capacity of these cells to differentiate. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical and Biophysical Research Communications
volume
256
issue
3
pages
557 - 563
publisher
Elsevier
external identifiers
  • pmid:10080936
  • scopus:0342668639
  • pmid:10080936
ISSN
1090-2104
DOI
10.1006/bbrc.1999.0314
language
English
LU publication?
yes
id
f4f404ff-4c1a-41d8-a794-b5c5d4fc4ff2 (old id 1114121)
date added to LUP
2016-04-01 16:43:28
date last changed
2022-03-30 17:49:27
@article{f4f404ff-4c1a-41d8-a794-b5c5d4fc4ff2,
  abstract     = {{The mammalian achaete-scute homologue, MASH-1, is crucial for early development of the sympathetic nervous system and is transiently expressed in sympathetic neuroblasts during embryogenesis. Here we report that the human homologue (HASH-1) was expressed in all analyzed cell lines (6/6) derived from the sympathetic nervous system tumor neuroblastoma. The majority of small-cell lung carcinoma (4/5) cell lines tested expressed HASH-1, while other nonneuronal/non-neuroendocrine cell lines were negative. Induced differentiation of neuroblastoma cells resulted in HASH-1 downregulation. This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of exogenous HASH-1 did not alter the capacity of the neuroblastoma cells to differentiate in response to differentiation-inducing agents. It is concluded that moderate HASH-1 expression does not compromise the capacity of these cells to differentiate.}},
  author       = {{Söderholm, H and Örtoft, Eva and Johansson, Irja and Ljungberg, June and Larsson, Christer and Axelson, Håkan and Påhlman, Sven}},
  issn         = {{1090-2104}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{557--563}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical and Biophysical Research Communications}},
  title        = {{Human achaete-scute homologue 1 (HASH-1) is downregulated in differentiating neuroblastoma cells}},
  url          = {{http://dx.doi.org/10.1006/bbrc.1999.0314}},
  doi          = {{10.1006/bbrc.1999.0314}},
  volume       = {{256}},
  year         = {{1999}},
}