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Reactive capsule formation around soft-tissue implants is related to cell necrosis

Rosengren, Anita ; Danielsen, Nils LU and Bjursten, Lars Magnus LU (1999) In Journal of Biomedical Materials Research 46(4). p.458-464
Abstract
Low-density polethylene disks with smooth or course surfaces were implanted in the abdominal wall of rats, and the tissue response was evaluated after 1, 6, or 12 weeks. Cell damage was detected by two different methods. Cells with increased membrane permeability could be identified using fluorescence microscopy by injection of propidium iodide prior to the killing of the rats. Second, cell death was verified by detection of DNA fragmentation. At 1 week a considerable number of the interfacial cells was stained with propidium iodide. Propidium-iodide-positive cells also were enriched at the edges of the disks irrespective of surface texture. The numbers of positive interfacial cells decreased markedly over time. Cells with DNA... (More)
Low-density polethylene disks with smooth or course surfaces were implanted in the abdominal wall of rats, and the tissue response was evaluated after 1, 6, or 12 weeks. Cell damage was detected by two different methods. Cells with increased membrane permeability could be identified using fluorescence microscopy by injection of propidium iodide prior to the killing of the rats. Second, cell death was verified by detection of DNA fragmentation. At 1 week a considerable number of the interfacial cells was stained with propidium iodide. Propidium-iodide-positive cells also were enriched at the edges of the disks irrespective of surface texture. The numbers of positive interfacial cells decreased markedly over time. Cells with DNA fragmentation initially displayed a scattered distribution; at later time points they appeared mainly in the outer portion of the enveloping capsule. The reactive capsule was thicker for the smooth surface, and there was a positive correlation between capsule thickness and propidium-iodide-positive cells at earlier implantation periods. The results suggest that the thickness of the reactive capsule is related to the extent of cell necrosis. It is suggested that the major initiator for this cell necrosis is mechanical shear since cell necrosis was found mainly in areas where mechanical shear could be expected. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
necrosis, apoptosis, macrophages, implant, surface texture
in
Journal of Biomedical Materials Research
volume
46
issue
4
pages
458 - 464
publisher
John Wiley and Sons
external identifiers
  • pmid:10398006
  • scopus:0033567867
ISSN
0021-9304
DOI
10.1002/(SICI)1097-4636(19990915)46:4<458::AID-JBM3>3.0.CO;2-I
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neural Interfaces (013212003), Bioimplant Research (013242910)
id
934d5e3b-3125-4a8e-9594-9c88d7695cb4 (old id 1114281)
date added to LUP
2016-04-01 11:36:25
date last changed
2021-01-06 05:18:51
@article{934d5e3b-3125-4a8e-9594-9c88d7695cb4,
  abstract     = {Low-density polethylene disks with smooth or course surfaces were implanted in the abdominal wall of rats, and the tissue response was evaluated after 1, 6, or 12 weeks. Cell damage was detected by two different methods. Cells with increased membrane permeability could be identified using fluorescence microscopy by injection of propidium iodide prior to the killing of the rats. Second, cell death was verified by detection of DNA fragmentation. At 1 week a considerable number of the interfacial cells was stained with propidium iodide. Propidium-iodide-positive cells also were enriched at the edges of the disks irrespective of surface texture. The numbers of positive interfacial cells decreased markedly over time. Cells with DNA fragmentation initially displayed a scattered distribution; at later time points they appeared mainly in the outer portion of the enveloping capsule. The reactive capsule was thicker for the smooth surface, and there was a positive correlation between capsule thickness and propidium-iodide-positive cells at earlier implantation periods. The results suggest that the thickness of the reactive capsule is related to the extent of cell necrosis. It is suggested that the major initiator for this cell necrosis is mechanical shear since cell necrosis was found mainly in areas where mechanical shear could be expected.},
  author       = {Rosengren, Anita and Danielsen, Nils and Bjursten, Lars Magnus},
  issn         = {0021-9304},
  language     = {eng},
  number       = {4},
  pages        = {458--464},
  publisher    = {John Wiley and Sons},
  series       = {Journal of Biomedical Materials Research},
  title        = {Reactive capsule formation around soft-tissue implants is related to cell necrosis},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-4636(19990915)46:4<458::AID-JBM3>3.0.CO;2-I},
  doi          = {10.1002/(SICI)1097-4636(19990915)46:4<458::AID-JBM3>3.0.CO;2-I},
  volume       = {46},
  year         = {1999},
}