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Binding of the potent allergen hexahydrophthalic anhydride in the mucosa of the upper respiratory and alimentary tract following single inhalation exposures in guinea pigs and rats

Lindh, Christian LU ; Jönsson, Bo A LU ; Johannesson, Gunvor LU ; Zhang, Xing-Dong; Welinder, Hans LU and Brittebo, E B (1999) In Toxicology 134(2-3). p.153-168
Abstract
Hexahydrophthalic anhydride (HHPA; CAS No. 13149-00-3) is a highly allergenic compound commonly used in the chemical industry. Guinea pigs and rats were exposed to [3H2]HHPA by inhalation for 3-8 h and were killed at various intervals during 7 days. The tissue distribution of non-volatile and covalently bound radioactivity was studied by autoradiography. Tissue bound radioactivity was mainly found in the mucosa of the upper respiratory airways, whereas negligible levels were observed in the lungs. In addition, tissue bound radioactivity was present in the gastrointestinal tract and conjunctiva. Moreover, in the cortex of the kidneys in rats, but not in guinea pigs, a low level of tissue bound radioactivity was found. The radioactivity in... (More)
Hexahydrophthalic anhydride (HHPA; CAS No. 13149-00-3) is a highly allergenic compound commonly used in the chemical industry. Guinea pigs and rats were exposed to [3H2]HHPA by inhalation for 3-8 h and were killed at various intervals during 7 days. The tissue distribution of non-volatile and covalently bound radioactivity was studied by autoradiography. Tissue bound radioactivity was mainly found in the mucosa of the upper respiratory airways, whereas negligible levels were observed in the lungs. In addition, tissue bound radioactivity was present in the gastrointestinal tract and conjunctiva. Moreover, in the cortex of the kidneys in rats, but not in guinea pigs, a low level of tissue bound radioactivity was found. The radioactivity in the tissues persisted for at least 7 days after the end of exposure. Plasma proteins and soluble proteins from trachea, lung, and kidney from [3H2]HHPA-exposed animals were separated by gel filtration. The radioactivity in dialysed plasma was mainly found in the same fractions as albumin. The soluble proteins from trachea, lung, and kidney in both rats and guinea pigs showed a similar pattern as found in blood. The radioactivity in dialysed plasma from both guinea pigs and rats seemed to decay according to a two-compartment model. The non-extractable binding of [3H2]HHPA in the upper respiratory airways and conjunctiva may be of relevance for symptoms in workers with allergy, since they mainly develop symptoms and signs from the nose and eyes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Inhalation, Hexahydrophthalic anhydride, Autoradiography, Distribution, Allergy
in
Toxicology
volume
134
issue
2-3
pages
153 - 168
publisher
Elsevier
external identifiers
  • pmid:10403634
  • scopus:0345161569
ISSN
0300-483X
DOI
10.1016/S0300-483X(99)00032-3
language
English
LU publication?
yes
id
a2afca27-5f09-4982-81bd-1c192a35423c (old id 1114506)
date added to LUP
2008-07-04 11:46:37
date last changed
2017-01-01 07:07:19
@article{a2afca27-5f09-4982-81bd-1c192a35423c,
  abstract     = {Hexahydrophthalic anhydride (HHPA; CAS No. 13149-00-3) is a highly allergenic compound commonly used in the chemical industry. Guinea pigs and rats were exposed to [3H2]HHPA by inhalation for 3-8 h and were killed at various intervals during 7 days. The tissue distribution of non-volatile and covalently bound radioactivity was studied by autoradiography. Tissue bound radioactivity was mainly found in the mucosa of the upper respiratory airways, whereas negligible levels were observed in the lungs. In addition, tissue bound radioactivity was present in the gastrointestinal tract and conjunctiva. Moreover, in the cortex of the kidneys in rats, but not in guinea pigs, a low level of tissue bound radioactivity was found. The radioactivity in the tissues persisted for at least 7 days after the end of exposure. Plasma proteins and soluble proteins from trachea, lung, and kidney from [3H2]HHPA-exposed animals were separated by gel filtration. The radioactivity in dialysed plasma was mainly found in the same fractions as albumin. The soluble proteins from trachea, lung, and kidney in both rats and guinea pigs showed a similar pattern as found in blood. The radioactivity in dialysed plasma from both guinea pigs and rats seemed to decay according to a two-compartment model. The non-extractable binding of [3H2]HHPA in the upper respiratory airways and conjunctiva may be of relevance for symptoms in workers with allergy, since they mainly develop symptoms and signs from the nose and eyes.},
  author       = {Lindh, Christian and Jönsson, Bo A and Johannesson, Gunvor and Zhang, Xing-Dong and Welinder, Hans and Brittebo, E B},
  issn         = {0300-483X},
  keyword      = {Inhalation,Hexahydrophthalic anhydride,Autoradiography,Distribution,Allergy},
  language     = {eng},
  number       = {2-3},
  pages        = {153--168},
  publisher    = {Elsevier},
  series       = {Toxicology},
  title        = {Binding of the potent allergen hexahydrophthalic anhydride in the mucosa of the upper respiratory and alimentary tract following single inhalation exposures in guinea pigs and rats},
  url          = {http://dx.doi.org/10.1016/S0300-483X(99)00032-3},
  volume       = {134},
  year         = {1999},
}