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Effects on the ciliated epithelium of protein D-producing and -nonproducing nontypeable Haemophilus influenzae in nasopharyngeal tissue cultures

Janson, Håkan LU ; Carlén, Birgitta LU ; Cervin, Anders LU ; Forsgren, Arne LU ; Magnusdottir, Anna Björk LU ; Lindberg, Sven LU and Runer, Thomas (1999) In Journal of Infectious Diseases 180(3). p.737-746
Abstract
A pair of isogenic, nontypeable Haemophilus influenzae strains, one expressing protein D and the other protein D-negative, was compared in their ability to cause damage in a human nasopharyngeal tissue culture model. Damage was assessed by measuring the ciliary beat frequency (CBF) of tissue specimens at 12 h intervals. Cultures inoculated with H. influenzae manifested a decrease in CBF beginning after 12 h, with a maximum decrease after 36 h. The impairment of ciliary function by the protein D-expressing strain was significantly greater than that caused by the protein D-negative mutant (P<.01). Tissue specimens examined by scanning and transmission electron microscopy after 24 h appeared normal. After 48 h of incubation, the protein... (More)
A pair of isogenic, nontypeable Haemophilus influenzae strains, one expressing protein D and the other protein D-negative, was compared in their ability to cause damage in a human nasopharyngeal tissue culture model. Damage was assessed by measuring the ciliary beat frequency (CBF) of tissue specimens at 12 h intervals. Cultures inoculated with H. influenzae manifested a decrease in CBF beginning after 12 h, with a maximum decrease after 36 h. The impairment of ciliary function by the protein D-expressing strain was significantly greater than that caused by the protein D-negative mutant (P<.01). Tissue specimens examined by scanning and transmission electron microscopy after 24 h appeared normal. After 48 h of incubation, the protein D-expressing strain caused a significant loss of cilia. These findings suggest that protein D is involved in the pathogenesis of upper respiratory tract infections due to nontypeable H. influenzae, probably by enhancing functional and morphological damage to cilia. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Infectious Diseases
volume
180
issue
3
pages
737 - 746
publisher
Oxford University Press
external identifiers
  • pmid:10438362
  • scopus:0032828504
ISSN
1537-6613
DOI
10.1086/314921
language
English
LU publication?
yes
id
24f888cd-0d1a-42e4-b94e-8b3a74ce2645 (old id 1114712)
date added to LUP
2008-07-04 15:34:30
date last changed
2017-01-01 06:48:52
@article{24f888cd-0d1a-42e4-b94e-8b3a74ce2645,
  abstract     = {A pair of isogenic, nontypeable Haemophilus influenzae strains, one expressing protein D and the other protein D-negative, was compared in their ability to cause damage in a human nasopharyngeal tissue culture model. Damage was assessed by measuring the ciliary beat frequency (CBF) of tissue specimens at 12 h intervals. Cultures inoculated with H. influenzae manifested a decrease in CBF beginning after 12 h, with a maximum decrease after 36 h. The impairment of ciliary function by the protein D-expressing strain was significantly greater than that caused by the protein D-negative mutant (P&lt;.01). Tissue specimens examined by scanning and transmission electron microscopy after 24 h appeared normal. After 48 h of incubation, the protein D-expressing strain caused a significant loss of cilia. These findings suggest that protein D is involved in the pathogenesis of upper respiratory tract infections due to nontypeable H. influenzae, probably by enhancing functional and morphological damage to cilia.},
  author       = {Janson, Håkan and Carlén, Birgitta and Cervin, Anders and Forsgren, Arne and Magnusdottir, Anna Björk and Lindberg, Sven and Runer, Thomas},
  issn         = {1537-6613},
  language     = {eng},
  number       = {3},
  pages        = {737--746},
  publisher    = {Oxford University Press},
  series       = {Journal of Infectious Diseases},
  title        = {Effects on the ciliated epithelium of protein D-producing and -nonproducing nontypeable Haemophilus influenzae in nasopharyngeal tissue cultures},
  url          = {http://dx.doi.org/10.1086/314921},
  volume       = {180},
  year         = {1999},
}