Effects on the ciliated epithelium of protein D-producing and -nonproducing nontypeable Haemophilus influenzae in nasopharyngeal tissue cultures
(1999) In Journal of Infectious Diseases 180(3). p.737-746- Abstract
- A pair of isogenic, nontypeable Haemophilus influenzae strains, one expressing protein D and the other protein D-negative, was compared in their ability to cause damage in a human nasopharyngeal tissue culture model. Damage was assessed by measuring the ciliary beat frequency (CBF) of tissue specimens at 12 h intervals. Cultures inoculated with H. influenzae manifested a decrease in CBF beginning after 12 h, with a maximum decrease after 36 h. The impairment of ciliary function by the protein D-expressing strain was significantly greater than that caused by the protein D-negative mutant (P<.01). Tissue specimens examined by scanning and transmission electron microscopy after 24 h appeared normal. After 48 h of incubation, the protein... (More)
- A pair of isogenic, nontypeable Haemophilus influenzae strains, one expressing protein D and the other protein D-negative, was compared in their ability to cause damage in a human nasopharyngeal tissue culture model. Damage was assessed by measuring the ciliary beat frequency (CBF) of tissue specimens at 12 h intervals. Cultures inoculated with H. influenzae manifested a decrease in CBF beginning after 12 h, with a maximum decrease after 36 h. The impairment of ciliary function by the protein D-expressing strain was significantly greater than that caused by the protein D-negative mutant (P<.01). Tissue specimens examined by scanning and transmission electron microscopy after 24 h appeared normal. After 48 h of incubation, the protein D-expressing strain caused a significant loss of cilia. These findings suggest that protein D is involved in the pathogenesis of upper respiratory tract infections due to nontypeable H. influenzae, probably by enhancing functional and morphological damage to cilia. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1114712
- author
- Janson, Håkan LU ; Carlén, Birgitta LU ; Cervin, Anders LU ; Forsgren, Arne LU ; Magnusdottir, Anna Björk LU ; Lindberg, Sven LU and Runer, Thomas
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Infectious Diseases
- volume
- 180
- issue
- 3
- pages
- 737 - 746
- publisher
- Oxford University Press
- external identifiers
-
- pmid:10438362
- scopus:0032828504
- pmid:10438362
- ISSN
- 1537-6613
- DOI
- 10.1086/314921
- language
- English
- LU publication?
- yes
- id
- 24f888cd-0d1a-42e4-b94e-8b3a74ce2645 (old id 1114712)
- date added to LUP
- 2016-04-01 15:51:44
- date last changed
- 2022-01-28 07:38:42
@article{24f888cd-0d1a-42e4-b94e-8b3a74ce2645, abstract = {{A pair of isogenic, nontypeable Haemophilus influenzae strains, one expressing protein D and the other protein D-negative, was compared in their ability to cause damage in a human nasopharyngeal tissue culture model. Damage was assessed by measuring the ciliary beat frequency (CBF) of tissue specimens at 12 h intervals. Cultures inoculated with H. influenzae manifested a decrease in CBF beginning after 12 h, with a maximum decrease after 36 h. The impairment of ciliary function by the protein D-expressing strain was significantly greater than that caused by the protein D-negative mutant (P<.01). Tissue specimens examined by scanning and transmission electron microscopy after 24 h appeared normal. After 48 h of incubation, the protein D-expressing strain caused a significant loss of cilia. These findings suggest that protein D is involved in the pathogenesis of upper respiratory tract infections due to nontypeable H. influenzae, probably by enhancing functional and morphological damage to cilia.}}, author = {{Janson, Håkan and Carlén, Birgitta and Cervin, Anders and Forsgren, Arne and Magnusdottir, Anna Björk and Lindberg, Sven and Runer, Thomas}}, issn = {{1537-6613}}, language = {{eng}}, number = {{3}}, pages = {{737--746}}, publisher = {{Oxford University Press}}, series = {{Journal of Infectious Diseases}}, title = {{Effects on the ciliated epithelium of protein D-producing and -nonproducing nontypeable Haemophilus influenzae in nasopharyngeal tissue cultures}}, url = {{http://dx.doi.org/10.1086/314921}}, doi = {{10.1086/314921}}, volume = {{180}}, year = {{1999}}, }