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Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen

Stenman, U H; Paus, E; Allard, W J; Andersson, I; Andres, C; Barnett, T R; Becker, Charlotte LU ; Belenky, A; Bellanger, L and Pellegrino, C M, et al. (1999) In Tumor Biology 20(Suppl. 1). p.1-12
Abstract
Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA... (More)
Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PSA, Prostate, Antibody, Workshop, ISOBM
in
Tumor Biology
volume
20
issue
Suppl. 1
pages
1 - 12
publisher
Springer
external identifiers
  • pmid:10628402
  • scopus:0032726502
ISSN
1423-0380
DOI
10.1159/000056523
language
English
LU publication?
yes
id
dd52b522-f26f-4f81-a0d7-cf93c0ba2804 (old id 1115131)
date added to LUP
2008-07-07 14:58:22
date last changed
2017-06-04 04:25:12
@article{dd52b522-f26f-4f81-a0d7-cf93c0ba2804,
  abstract     = {Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.},
  author       = {Stenman, U H and Paus, E and Allard, W J and Andersson, I and Andres, C and Barnett, T R and Becker, Charlotte and Belenky, A and Bellanger, L and Pellegrino, C M and Bormer, O P and Davis, G and Dowell, B and Grauer, L S and Jette, D C and Karlsson, B and Kreutz, F T and van der Kwast, T M and Lauren, L and Leinimaa, M and Leinonen, J and Lilja, Hans and Linton, H J and Nap, M and Nilsson, O and Ng, P C and Nustad, K and Peter, A and Pettersson, K and Piironen, T and Rapp, J and Rittenhouse, H G and Rye, P D and Seguin, P and Slota, J and Sokoloff, R L and Suresh, M R and Very, D L and Wang, T J and Wigheden, Ingrid and Wolfert, R L and Yeung, K K and Zhang, W-M and Zhou, Z and Hilgers, J},
  issn         = {1423-0380},
  keyword      = {PSA,Prostate,Antibody,Workshop,ISOBM},
  language     = {eng},
  number       = {Suppl. 1},
  pages        = {1--12},
  publisher    = {Springer},
  series       = {Tumor Biology},
  title        = {Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen},
  url          = {http://dx.doi.org/10.1159/000056523},
  volume       = {20},
  year         = {1999},
}