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HASH-1 and E2-2 are expressed in human neuroblastoma cells and form a functional complex

Persson, Paula LU ; Jögi, Annika LU ; Grynfeld, Anna LU ; Påhlman, Sven LU and Axelson, Håkan LU (2000) In Biochemical and Biophysical Research Communications 274(1). p.22-31
Abstract
The basic helix-loop-helix (bHLH) transcription factor mammalian achaete-scute homolog-1 (MASH-1 in mouse and HASH-1 in human) is essential for proper development of olfactory and most peripheral autonomic neurons, and for the formation of distinct neuronal circuits within the central nervous system. We have previously shown that HASH-1 is expressed in neuroblastoma tumors and cell lines, and in this study we have used the yeast two-hybrid system to isolate HASH-1 interacting proteins from a human neuroblastoma cDNA library. Two of the isolated clones contained cDNA from the E2-2 gene (also known as ITF2/SEF2-1). We show that E2-2 interacts with HASH-1 in both yeast and mammalian cells. The HASH-1/E2-2 complex binds an E-box (CACCTG) in... (More)
The basic helix-loop-helix (bHLH) transcription factor mammalian achaete-scute homolog-1 (MASH-1 in mouse and HASH-1 in human) is essential for proper development of olfactory and most peripheral autonomic neurons, and for the formation of distinct neuronal circuits within the central nervous system. We have previously shown that HASH-1 is expressed in neuroblastoma tumors and cell lines, and in this study we have used the yeast two-hybrid system to isolate HASH-1 interacting proteins from a human neuroblastoma cDNA library. Two of the isolated clones contained cDNA from the E2-2 gene (also known as ITF2/SEF2-1). We show that E2-2 interacts with HASH-1 in both yeast and mammalian cells. The HASH-1/E2-2 complex binds an E-box (CACCTG) in vitro, and transactivates an E-box containing reporter construct in vivo. Furthermore, E2-2 seems to be one of the major HASH-1 interacting proteins in extracts from neuroblastoma cells. In conclusion, E2-2 forms a functional complex with HASH-1, and might therefore be involved in the development of specific parts of the central and peripheral nervous systems. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
SEF2-1, neuroblastoma, HASH-1, sympathetic nervous system, E2-2, bHLH, differentiation, yeast two-hybrid
in
Biochemical and Biophysical Research Communications
volume
274
issue
1
pages
22 - 31
publisher
Elsevier
external identifiers
  • pmid:10903890
  • scopus:0343628046
ISSN
1090-2104
DOI
10.1006/bbrc.2000.3090
language
English
LU publication?
yes
id
d8cfe412-bde2-49d0-8dfb-f4aab7031cb2 (old id 1116257)
date added to LUP
2008-07-01 10:58:16
date last changed
2017-05-21 04:15:00
@article{d8cfe412-bde2-49d0-8dfb-f4aab7031cb2,
  abstract     = {The basic helix-loop-helix (bHLH) transcription factor mammalian achaete-scute homolog-1 (MASH-1 in mouse and HASH-1 in human) is essential for proper development of olfactory and most peripheral autonomic neurons, and for the formation of distinct neuronal circuits within the central nervous system. We have previously shown that HASH-1 is expressed in neuroblastoma tumors and cell lines, and in this study we have used the yeast two-hybrid system to isolate HASH-1 interacting proteins from a human neuroblastoma cDNA library. Two of the isolated clones contained cDNA from the E2-2 gene (also known as ITF2/SEF2-1). We show that E2-2 interacts with HASH-1 in both yeast and mammalian cells. The HASH-1/E2-2 complex binds an E-box (CACCTG) in vitro, and transactivates an E-box containing reporter construct in vivo. Furthermore, E2-2 seems to be one of the major HASH-1 interacting proteins in extracts from neuroblastoma cells. In conclusion, E2-2 forms a functional complex with HASH-1, and might therefore be involved in the development of specific parts of the central and peripheral nervous systems.},
  author       = {Persson, Paula and Jögi, Annika and Grynfeld, Anna and Påhlman, Sven and Axelson, Håkan},
  issn         = {1090-2104},
  keyword      = {SEF2-1,neuroblastoma,HASH-1,sympathetic nervous system,E2-2,bHLH,differentiation,yeast two-hybrid},
  language     = {eng},
  number       = {1},
  pages        = {22--31},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {HASH-1 and E2-2 are expressed in human neuroblastoma cells and form a functional complex},
  url          = {http://dx.doi.org/10.1006/bbrc.2000.3090},
  volume       = {274},
  year         = {2000},
}