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Locus-specific multifluor FISH analysis allows physical characterization of complex chromosome abnormalities in neoplasia

Gisselsson Nord, David LU ; Mandahl, Nils LU ; Pålsson, Eva LU ; Gorunova, Ludmila LU and Höglund, Mattias LU (2000) In Genes, Chromosomes and Cancer 28(3). p.347-352
Abstract
Novel techniques in molecular cytogenetics have radically improved the ability to characterize genetic changes in neoplastic cells. In parallel, a rapid development in high-throughput genomics has resulted in detailed physical maps of the human genome. Combining these two fields, we have developed a method for the simultaneous visualization of several physically defined segments along a chromosome. Seven YAC clones and one subtelomeric cosmid clone from chromosome 12 were labeled with unique combinations of four fluors and hybridized to metaphase chromosomes from neoplastic cells. In a uterine leiomyoma and a myxoid liposarcoma with translocations 12;14 and 12;16, the breakpoints in chromosome 12 could be localized to the HMGIC and CHOP... (More)
Novel techniques in molecular cytogenetics have radically improved the ability to characterize genetic changes in neoplastic cells. In parallel, a rapid development in high-throughput genomics has resulted in detailed physical maps of the human genome. Combining these two fields, we have developed a method for the simultaneous visualization of several physically defined segments along a chromosome. Seven YAC clones and one subtelomeric cosmid clone from chromosome 12 were labeled with unique combinations of four fluors and hybridized to metaphase chromosomes from neoplastic cells. In a uterine leiomyoma and a myxoid liposarcoma with translocations 12;14 and 12;16, the breakpoints in chromosome 12 could be localized to the HMGIC and CHOP regions, respectively. In the other tumors, more complex aberrations were visualized, including two inversions in 12q with a common breakpoint between MDM2 and D12S332 in a pleomorphic adenoma, amplification of MDM2 and CDK4 in ring chromosomes from a malignant fibrous histiocytoma, and amplification of KRAS2 together with other unbalanced rearrangements in two pancreatic adenocarcinomas. Combinatorially labeled single-copy probes may thus simultaneously provide physical localization of breakpoints and an overview of complex structural rearrangements. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Genes, Chromosomes and Cancer
volume
28
issue
3
pages
347 - 352
publisher
John Wiley & Sons
external identifiers
  • pmid:10862042
  • scopus:0034094217
ISSN
1045-2257
DOI
10.1002/1098-2264(200007)28:3<347::AID-GCC14>3.0.CO;2-2
language
English
LU publication?
yes
id
b5da5f00-2754-44de-ad76-d240c37d80fa (old id 1116723)
date added to LUP
2008-07-01 15:51:46
date last changed
2017-04-09 03:34:22
@article{b5da5f00-2754-44de-ad76-d240c37d80fa,
  abstract     = {Novel techniques in molecular cytogenetics have radically improved the ability to characterize genetic changes in neoplastic cells. In parallel, a rapid development in high-throughput genomics has resulted in detailed physical maps of the human genome. Combining these two fields, we have developed a method for the simultaneous visualization of several physically defined segments along a chromosome. Seven YAC clones and one subtelomeric cosmid clone from chromosome 12 were labeled with unique combinations of four fluors and hybridized to metaphase chromosomes from neoplastic cells. In a uterine leiomyoma and a myxoid liposarcoma with translocations 12;14 and 12;16, the breakpoints in chromosome 12 could be localized to the HMGIC and CHOP regions, respectively. In the other tumors, more complex aberrations were visualized, including two inversions in 12q with a common breakpoint between MDM2 and D12S332 in a pleomorphic adenoma, amplification of MDM2 and CDK4 in ring chromosomes from a malignant fibrous histiocytoma, and amplification of KRAS2 together with other unbalanced rearrangements in two pancreatic adenocarcinomas. Combinatorially labeled single-copy probes may thus simultaneously provide physical localization of breakpoints and an overview of complex structural rearrangements.},
  author       = {Gisselsson Nord, David and Mandahl, Nils and Pålsson, Eva and Gorunova, Ludmila and Höglund, Mattias},
  issn         = {1045-2257},
  language     = {eng},
  number       = {3},
  pages        = {347--352},
  publisher    = {John Wiley & Sons},
  series       = {Genes, Chromosomes and Cancer},
  title        = {Locus-specific multifluor FISH analysis allows physical characterization of complex chromosome abnormalities in neoplasia},
  url          = {http://dx.doi.org/10.1002/1098-2264(200007)28:3<347::AID-GCC14>3.0.CO;2-2},
  volume       = {28},
  year         = {2000},
}