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Use of chemically extracted muscle grafts to repair extended nerve defects in rats

Liu, Xiao-Lin; Arai, Takeru; Sondell, Marianne; Lundborg, Göran LU ; Kanje, Martin LU and Dahlin, Lars LU (2001) In Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00 35(4). p.337-345
Abstract
Nerve regeneration, measured as axonal outgrowth, Schwann cell migration, macrophage invasion, and neovascularisation, was compared after repair of a 15 mm gap in rats' sciatic nerves using autologous muscle grafts made acellular either by freezing and thawing or by chemical extraction. Both extracted and freeze-thawed acellular muscle grafts could be used to bridge the defect. However, axons and Schwann cells, as shown by immunohistochemical staining for neurofilaments and S-100 protein, respectively, grew faster into the extracted muscle grafts than into the freeze-thawed acellular muscle grafts and somewhat more axons were observed in the former graft. There were no significant differences between the two graft types with respect to... (More)
Nerve regeneration, measured as axonal outgrowth, Schwann cell migration, macrophage invasion, and neovascularisation, was compared after repair of a 15 mm gap in rats' sciatic nerves using autologous muscle grafts made acellular either by freezing and thawing or by chemical extraction. Both extracted and freeze-thawed acellular muscle grafts could be used to bridge the defect. However, axons and Schwann cells, as shown by immunohistochemical staining for neurofilaments and S-100 protein, respectively, grew faster into the extracted muscle grafts than into the freeze-thawed acellular muscle grafts and somewhat more axons were observed in the former graft. There were no significant differences between the two graft types with respect to neovascularisation as showed by staining for endothelial alkaline phosphatase, and limited differences concerning invasion of macrophages (ED1 and ED2) as detected by immunocytochemistry. The results showed that chemically extracted muscle grafts could be used to bridge an extended nerve defect and that such grafts in some aspects were superior to freeze-thawed muscle grafts for extended gaps. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Muscle, Graft, Acellular, Nerve, Regeneration, Schwann, Cell, Macrophages, Immunocytochemistry, Axons, Repair
in
Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00
volume
35
issue
4
pages
337 - 345
publisher
Taylor & Francis
external identifiers
  • wos:000173155800001
  • scopus:0035663324
ISSN
1651-2073
DOI
10.1080/028443101317149291
language
English
LU publication?
yes
id
f195a240-729d-404c-af84-13cb0b9c08dd (old id 1118522)
date added to LUP
2008-07-04 10:47:07
date last changed
2018-01-07 08:40:02
@article{f195a240-729d-404c-af84-13cb0b9c08dd,
  abstract     = {Nerve regeneration, measured as axonal outgrowth, Schwann cell migration, macrophage invasion, and neovascularisation, was compared after repair of a 15 mm gap in rats' sciatic nerves using autologous muscle grafts made acellular either by freezing and thawing or by chemical extraction. Both extracted and freeze-thawed acellular muscle grafts could be used to bridge the defect. However, axons and Schwann cells, as shown by immunohistochemical staining for neurofilaments and S-100 protein, respectively, grew faster into the extracted muscle grafts than into the freeze-thawed acellular muscle grafts and somewhat more axons were observed in the former graft. There were no significant differences between the two graft types with respect to neovascularisation as showed by staining for endothelial alkaline phosphatase, and limited differences concerning invasion of macrophages (ED1 and ED2) as detected by immunocytochemistry. The results showed that chemically extracted muscle grafts could be used to bridge an extended nerve defect and that such grafts in some aspects were superior to freeze-thawed muscle grafts for extended gaps.},
  author       = {Liu, Xiao-Lin and Arai, Takeru and Sondell, Marianne and Lundborg, Göran and Kanje, Martin and Dahlin, Lars},
  issn         = {1651-2073},
  keyword      = {Muscle,Graft,Acellular,Nerve,Regeneration,Schwann,Cell,Macrophages,Immunocytochemistry,Axons,Repair},
  language     = {eng},
  number       = {4},
  pages        = {337--345},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00},
  title        = {Use of chemically extracted muscle grafts to repair extended nerve defects in rats},
  url          = {http://dx.doi.org/10.1080/028443101317149291},
  volume       = {35},
  year         = {2001},
}