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Comparison of some different methods for analysis of thyroid autoantibodies: Importance of thyroglobulin autoantibodies

Lindberg, Bengt LU ; Svensson, Johan LU ; Ericsson, Ulla-Britt; Nilsson, Paul; Svenonius, Eivor and Ivarsson, Sten LU (2001) In Thyroid 11(3). p.265-269
Abstract
Blood samples from 141 children and adolescents were used to evaluate differences between commercial kits and radioimmunoassay (RIA) methods for detecting thyroid autoantibodies. Thyroglobulin autoantibodies (Tg-Ab) were analyzed with a hemagglutination kit and a RIA; thyroid peroxidase autoantibodies (TPO-Ab) were measured with a gelagglutination assay and a RIA. The result; of the antibody tests were compared with thyroid function tests (triiodothyronine [T-3] thyroxine [T-4], thyrotropin [TSH]) and with the results of ultrasound of the thyroid in antibody-positive patients. The correlation of antibody levels between the two methods was higher for TPO-Ab than for Tg-Ab. Moderate to high levels of TPO-Ab correlated to elevated TSH levels.... (More)
Blood samples from 141 children and adolescents were used to evaluate differences between commercial kits and radioimmunoassay (RIA) methods for detecting thyroid autoantibodies. Thyroglobulin autoantibodies (Tg-Ab) were analyzed with a hemagglutination kit and a RIA; thyroid peroxidase autoantibodies (TPO-Ab) were measured with a gelagglutination assay and a RIA. The result; of the antibody tests were compared with thyroid function tests (triiodothyronine [T-3] thyroxine [T-4], thyrotropin [TSH]) and with the results of ultrasound of the thyroid in antibody-positive patients. The correlation of antibody levels between the two methods was higher for TPO-Ab than for Tg-Ab. Moderate to high levels of TPO-Ab correlated to elevated TSH levels. Auto immune thyroiditis (AIT) was found in 6 of the 141 children. The RIA-based thyroglobulin assay was the only test that identified autoantibodies in all 6 cases. in contrast, the hemagglutination kit thyroglobulin assay failed to identify 4 of the 6 AIT cases. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Thyroid
volume
11
issue
3
pages
265 - 269
publisher
Mary Ann Liebert, Inc.
external identifiers
  • wos:000168143700010
  • scopus:0035050878
ISSN
1557-9077
DOI
10.1089/105072501750159697
language
English
LU publication?
yes
id
028f47a8-7116-4e48-b02d-ef24761af957 (old id 1119312)
date added to LUP
2008-07-03 11:52:43
date last changed
2018-08-05 04:08:07
@article{028f47a8-7116-4e48-b02d-ef24761af957,
  abstract     = {Blood samples from 141 children and adolescents were used to evaluate differences between commercial kits and radioimmunoassay (RIA) methods for detecting thyroid autoantibodies. Thyroglobulin autoantibodies (Tg-Ab) were analyzed with a hemagglutination kit and a RIA; thyroid peroxidase autoantibodies (TPO-Ab) were measured with a gelagglutination assay and a RIA. The result; of the antibody tests were compared with thyroid function tests (triiodothyronine [T-3] thyroxine [T-4], thyrotropin [TSH]) and with the results of ultrasound of the thyroid in antibody-positive patients. The correlation of antibody levels between the two methods was higher for TPO-Ab than for Tg-Ab. Moderate to high levels of TPO-Ab correlated to elevated TSH levels. Auto immune thyroiditis (AIT) was found in 6 of the 141 children. The RIA-based thyroglobulin assay was the only test that identified autoantibodies in all 6 cases. in contrast, the hemagglutination kit thyroglobulin assay failed to identify 4 of the 6 AIT cases.},
  author       = {Lindberg, Bengt and Svensson, Johan and Ericsson, Ulla-Britt and Nilsson, Paul and Svenonius, Eivor and Ivarsson, Sten},
  issn         = {1557-9077},
  language     = {eng},
  number       = {3},
  pages        = {265--269},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {Thyroid},
  title        = {Comparison of some different methods for analysis of thyroid autoantibodies: Importance of thyroglobulin autoantibodies},
  url          = {http://dx.doi.org/10.1089/105072501750159697},
  volume       = {11},
  year         = {2001},
}