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Direct cytoplasmic CA(2+) responses to gastrin-releasing peptide in single beta cells

Karlsson, Sven ; Sundler, Frank LU and Ahrén, Bo LU (2001) In Biochemical and Biophysical Research Communications 280(3). p.610-614
Abstract
The neuropeptide gastrin releasing peptide (GRP) stimulates insulin secretion and induces oscillations of the cytoplasmic Ca(2+) concentration ([Ca(2+)](cyt)) in clonal insulinoma cells. It is not known whether GRP affects [Ca(2+)](cyt) in normal beta cells. We investigated, in single, normal, mouse islet beta cells, the effects of GRP on [Ca(2+)](cyt), by dual wavelength spectrophotofluorometry. Beta cells were identified by their typical response to glucose or tolbutamide. At 15 mM glucose, GRP (100 nM) evoked an immediate [Ca(2+)](cyt) transient to 423 +/- 48 nM compared to 126 +/- 18 nM before GRP (P < 0.001). After the initial transient, [Ca(2+)](cyt) exhibited either a sustained elevation or oscillations. At 3.3 mM glucose, in... (More)
The neuropeptide gastrin releasing peptide (GRP) stimulates insulin secretion and induces oscillations of the cytoplasmic Ca(2+) concentration ([Ca(2+)](cyt)) in clonal insulinoma cells. It is not known whether GRP affects [Ca(2+)](cyt) in normal beta cells. We investigated, in single, normal, mouse islet beta cells, the effects of GRP on [Ca(2+)](cyt), by dual wavelength spectrophotofluorometry. Beta cells were identified by their typical response to glucose or tolbutamide. At 15 mM glucose, GRP (100 nM) evoked an immediate [Ca(2+)](cyt) transient to 423 +/- 48 nM compared to 126 +/- 18 nM before GRP (P < 0.001). After the initial transient, [Ca(2+)](cyt) exhibited either a sustained elevation or oscillations. At 3.3 mM glucose, in cells with a non-oscillating [Ca(2+)](cyt), GRP stimulated a prompt increase in [Ca(2+)](cyt) (from 60 +/- 6 to 285 +/- 30 nM, P = 0.024) followed by either a sustained increase in [Ca(2+)](cyt) or [Ca(2+)](cyt) oscillations. We conclude that GRP promptly elevates [Ca(2+)](cyt) by a direct action in normal mouse pancreatic beta cells. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
islets of Langerhans, insulin secretion, neuropeptide, phospholipase C, Ca2+ oscillations, GRP
in
Biochemical and Biophysical Research Communications
volume
280
issue
3
pages
610 - 614
publisher
Elsevier
external identifiers
  • pmid:11162563
  • scopus:0034815445
ISSN
1090-2104
DOI
10.1006/bbrc.2000.4165
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuroendocrine Cell Biology (013212008), Medicine (Lund) (013230025)
id
59764891-5de5-4e84-88f4-e15e8926d48c (old id 1120382)
date added to LUP
2016-04-01 16:34:05
date last changed
2022-01-28 20:34:06
@article{59764891-5de5-4e84-88f4-e15e8926d48c,
  abstract     = {{The neuropeptide gastrin releasing peptide (GRP) stimulates insulin secretion and induces oscillations of the cytoplasmic Ca(2+) concentration ([Ca(2+)](cyt)) in clonal insulinoma cells. It is not known whether GRP affects [Ca(2+)](cyt) in normal beta cells. We investigated, in single, normal, mouse islet beta cells, the effects of GRP on [Ca(2+)](cyt), by dual wavelength spectrophotofluorometry. Beta cells were identified by their typical response to glucose or tolbutamide. At 15 mM glucose, GRP (100 nM) evoked an immediate [Ca(2+)](cyt) transient to 423 +/- 48 nM compared to 126 +/- 18 nM before GRP (P &lt; 0.001). After the initial transient, [Ca(2+)](cyt) exhibited either a sustained elevation or oscillations. At 3.3 mM glucose, in cells with a non-oscillating [Ca(2+)](cyt), GRP stimulated a prompt increase in [Ca(2+)](cyt) (from 60 +/- 6 to 285 +/- 30 nM, P = 0.024) followed by either a sustained increase in [Ca(2+)](cyt) or [Ca(2+)](cyt) oscillations. We conclude that GRP promptly elevates [Ca(2+)](cyt) by a direct action in normal mouse pancreatic beta cells.}},
  author       = {{Karlsson, Sven and Sundler, Frank and Ahrén, Bo}},
  issn         = {{1090-2104}},
  keywords     = {{islets of Langerhans; insulin secretion; neuropeptide; phospholipase C; Ca2+ oscillations; GRP}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{610--614}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical and Biophysical Research Communications}},
  title        = {{Direct cytoplasmic CA(2+) responses to gastrin-releasing peptide in single beta cells}},
  url          = {{http://dx.doi.org/10.1006/bbrc.2000.4165}},
  doi          = {{10.1006/bbrc.2000.4165}},
  volume       = {{280}},
  year         = {{2001}},
}