Protein D expression promotes the adherence and internalization of non-typeable Haemophilus influenzae into human monocytic cells
(2001) In Microbial Pathogenesis 31(3). p.151-158- Abstract
- Protein D, having a glycerol-3-phosphodiester phosphodiesterase activity, is found at the surface of all Haemophilus influenzae strains and is a possible virulence factor. In the present study, the involvement of protein D in the entry of NTHi into human monocytic cells is reported. Primary monocytes and the monocytic cell lines U-937 and THP-1 were infected with NTHi strain 772 and the mutant 772 Delta hpd 1 (lacking the gene for protein D). NTHi 772 adhered to and entered monocytic cells up to four-fold more efficiently compared to 772 Delta hpd 1. When an Escherichia coli transformant expressing protein D was incubated with monocytic cells, the number of intracellular bacteria increased 1.6-fold compared to protein D-deficient controls.... (More)
- Protein D, having a glycerol-3-phosphodiester phosphodiesterase activity, is found at the surface of all Haemophilus influenzae strains and is a possible virulence factor. In the present study, the involvement of protein D in the entry of NTHi into human monocytic cells is reported. Primary monocytes and the monocytic cell lines U-937 and THP-1 were infected with NTHi strain 772 and the mutant 772 Delta hpd 1 (lacking the gene for protein D). NTHi 772 adhered to and entered monocytic cells up to four-fold more efficiently compared to 772 Delta hpd 1. When an Escherichia coli transformant expressing protein D was incubated with monocytic cells, the number of intracellular bacteria increased 1.6-fold compared to protein D-deficient controls. Any correlation between internalization and phosphorylcholine expression was not detected. In conclusion, our data suggest that surface-expressed protein D promotes the adherence of NTHi to human monocytes leading to a higher number of internalized bacteria. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1120776
- author
- Lazou Ahrén, Irini
LU
; Janson, Håkan
LU
; Forsgren, Arne
LU
and Riesbeck, Kristian
LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- non-typeable Haemophilus influenzae, internalization, glycerophosphodiester phosphodiesterase, protein D, monocytes
- in
- Microbial Pathogenesis
- volume
- 31
- issue
- 3
- pages
- 151 - 158
- publisher
- Academic Press
- external identifiers
-
- pmid:11500100
- scopus:0034866228
- ISSN
- 1096-1208
- DOI
- 10.1006/mpat.2001.0456
- language
- English
- LU publication?
- yes
- id
- c100d656-7c1a-4c82-8a1c-571bafa41783 (old id 1120776)
- date added to LUP
- 2016-04-01 12:27:04
- date last changed
- 2022-01-27 03:55:09
@article{c100d656-7c1a-4c82-8a1c-571bafa41783, abstract = {{Protein D, having a glycerol-3-phosphodiester phosphodiesterase activity, is found at the surface of all Haemophilus influenzae strains and is a possible virulence factor. In the present study, the involvement of protein D in the entry of NTHi into human monocytic cells is reported. Primary monocytes and the monocytic cell lines U-937 and THP-1 were infected with NTHi strain 772 and the mutant 772 Delta hpd 1 (lacking the gene for protein D). NTHi 772 adhered to and entered monocytic cells up to four-fold more efficiently compared to 772 Delta hpd 1. When an Escherichia coli transformant expressing protein D was incubated with monocytic cells, the number of intracellular bacteria increased 1.6-fold compared to protein D-deficient controls. Any correlation between internalization and phosphorylcholine expression was not detected. In conclusion, our data suggest that surface-expressed protein D promotes the adherence of NTHi to human monocytes leading to a higher number of internalized bacteria.}}, author = {{Lazou Ahrén, Irini and Janson, Håkan and Forsgren, Arne and Riesbeck, Kristian}}, issn = {{1096-1208}}, keywords = {{non-typeable Haemophilus influenzae; internalization; glycerophosphodiester phosphodiesterase; protein D; monocytes}}, language = {{eng}}, number = {{3}}, pages = {{151--158}}, publisher = {{Academic Press}}, series = {{Microbial Pathogenesis}}, title = {{Protein D expression promotes the adherence and internalization of non-typeable Haemophilus influenzae into human monocytic cells}}, url = {{http://dx.doi.org/10.1006/mpat.2001.0456}}, doi = {{10.1006/mpat.2001.0456}}, volume = {{31}}, year = {{2001}}, }