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Association of chondroadherin with collagen type II

Månsson, Bengt LU ; Wenglén, Christina LU ; Mörgelin, Matthias LU ; Saxne, Tore LU and Heinegård, Dick LU (2001) In Journal of Biological Chemistry 276(35). p.32883-32888
Abstract
Chondroadherin is a cell binding, leucine-rich repeat protein found in the territorial matrix of articular cartilage. Several members of the leucine-rich repeat protein family present in the extracellular matrix of e.g. cartilage have been shown to interact with collagen and influence collagen fibrillogenesis. We show that complexes of monomeric collagen type II and chondroadherin can be released under non-denaturing conditions from articular cartilage treated with p-aminophenylmercuric acetate to activate resident matrix metalloproteinases. Purified complexes as well as complexes formed in vitro between recombinant chondroadherin and collagen type II were studied by electron microscopy. Chondroadherin was shown to bind to two sites on... (More)
Chondroadherin is a cell binding, leucine-rich repeat protein found in the territorial matrix of articular cartilage. Several members of the leucine-rich repeat protein family present in the extracellular matrix of e.g. cartilage have been shown to interact with collagen and influence collagen fibrillogenesis. We show that complexes of monomeric collagen type II and chondroadherin can be released under non-denaturing conditions from articular cartilage treated with p-aminophenylmercuric acetate to activate resident matrix metalloproteinases. Purified complexes as well as complexes formed in vitro between recombinant chondroadherin and collagen type II were studied by electron microscopy. Chondroadherin was shown to bind to two sites on collagen type II. The interaction was characterized by surface plasmon resonance analysis showing K(D) values in the nanomolar range. Both chondroadherin and collagen interact with chondrocytes, partly via the same receptor, but give rise to different cellular responses. By also interacting with each other, a complex system is created which may be of functional importance for the communication between the cells and its surrounding matrix and/or in the regulation of collagen fibril assembly. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
276
issue
35
pages
32883 - 32888
publisher
ASBMB
external identifiers
  • pmid:11445564
  • scopus:0035980090
ISSN
1083-351X
DOI
10.1074/jbc.M101680200
language
English
LU publication?
yes
id
3094a151-3926-413a-8215-037bad539a24 (old id 1121080)
date added to LUP
2008-07-04 14:33:13
date last changed
2018-08-05 03:29:59
@article{3094a151-3926-413a-8215-037bad539a24,
  abstract     = {Chondroadherin is a cell binding, leucine-rich repeat protein found in the territorial matrix of articular cartilage. Several members of the leucine-rich repeat protein family present in the extracellular matrix of e.g. cartilage have been shown to interact with collagen and influence collagen fibrillogenesis. We show that complexes of monomeric collagen type II and chondroadherin can be released under non-denaturing conditions from articular cartilage treated with p-aminophenylmercuric acetate to activate resident matrix metalloproteinases. Purified complexes as well as complexes formed in vitro between recombinant chondroadherin and collagen type II were studied by electron microscopy. Chondroadherin was shown to bind to two sites on collagen type II. The interaction was characterized by surface plasmon resonance analysis showing K(D) values in the nanomolar range. Both chondroadherin and collagen interact with chondrocytes, partly via the same receptor, but give rise to different cellular responses. By also interacting with each other, a complex system is created which may be of functional importance for the communication between the cells and its surrounding matrix and/or in the regulation of collagen fibril assembly.},
  author       = {Månsson, Bengt and Wenglén, Christina and Mörgelin, Matthias and Saxne, Tore and Heinegård, Dick},
  issn         = {1083-351X},
  language     = {eng},
  number       = {35},
  pages        = {32883--32888},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Association of chondroadherin with collagen type II},
  url          = {http://dx.doi.org/10.1074/jbc.M101680200},
  volume       = {276},
  year         = {2001},
}