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Dedifferentiation of serous ovarian cancer from cystic to solid tumors is associated with increased expression of mRNA for urokinase plasminogen activator (uPA), its receptor (uPAR) and its inhibitor (PAI-1)

Borgfeldt, Christer LU ; Hansson, Stefan LU ; Gustavsson, Barbro LU ; Måsbäck, Anna LU and Casslén, Bertil LU (2001) In International Journal of Cancer 92(4). p.497-502
Abstract
The plasminogen activating system is involved in tumor growth and metastasis by degradation of extracellular matrix, and modulation of cell adhesion and migration. Benign and well-differentiated malignant ovarian tumors present as cystic lesions with preserved glandular morphology, whereas poorly differentiated tumors and metastases are solid with characteristic absence of glandular morphology. We analyzed the mRNAs for urokinase plasminogen activator (uPA), its receptor (uPAR), and inhibitor (PAI-1) in serous ovarian tumors by in situ hybridization and by densitometric scanning of Northern blots prepared from tissue extracts. The mRNA expressing cells in the in situ hybridization sections were evaluated and counted by two different... (More)
The plasminogen activating system is involved in tumor growth and metastasis by degradation of extracellular matrix, and modulation of cell adhesion and migration. Benign and well-differentiated malignant ovarian tumors present as cystic lesions with preserved glandular morphology, whereas poorly differentiated tumors and metastases are solid with characteristic absence of glandular morphology. We analyzed the mRNAs for urokinase plasminogen activator (uPA), its receptor (uPAR), and inhibitor (PAI-1) in serous ovarian tumors by in situ hybridization and by densitometric scanning of Northern blots prepared from tissue extracts. The mRNA expressing cells in the in situ hybridization sections were evaluated and counted by two different observers. The number of mRNA expressing cells for uPA, uPAR and PAI-1 were all significantly increased in solid as compared with cystic malignant tumors. The increased expression of all three mRNA species was mainly located in the stroma of poorly differentiated tumors and metastases. Apart from being expressed in the stroma of these tumors, uPAR mRNA was also expressed by tumor cells located along the stromal/epithelial boarder. In addition, the tumor tissue content of uPA, uPAR and PAI-1 mRNAs as measured by Northern blots were higher in the solid as compared with the cystic tumors. Increased expression of uPA, uPAR and PAI-1 genes in the solid tumors suggest a correlation with a more aggressive phenotype. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
human, ovarian neoplasm, mRNA analysis, urokinase, plasminogen activation
in
International Journal of Cancer
volume
92
issue
4
pages
497 - 502
publisher
John Wiley & Sons
external identifiers
  • pmid:11304683
  • scopus:0035874046
ISSN
0020-7136
DOI
10.1002/ijc.1215
language
English
LU publication?
yes
id
4191771b-78b2-4788-9883-1493f5668704 (old id 1121182)
date added to LUP
2008-06-25 12:46:51
date last changed
2018-05-29 11:41:40
@article{4191771b-78b2-4788-9883-1493f5668704,
  abstract     = {The plasminogen activating system is involved in tumor growth and metastasis by degradation of extracellular matrix, and modulation of cell adhesion and migration. Benign and well-differentiated malignant ovarian tumors present as cystic lesions with preserved glandular morphology, whereas poorly differentiated tumors and metastases are solid with characteristic absence of glandular morphology. We analyzed the mRNAs for urokinase plasminogen activator (uPA), its receptor (uPAR), and inhibitor (PAI-1) in serous ovarian tumors by in situ hybridization and by densitometric scanning of Northern blots prepared from tissue extracts. The mRNA expressing cells in the in situ hybridization sections were evaluated and counted by two different observers. The number of mRNA expressing cells for uPA, uPAR and PAI-1 were all significantly increased in solid as compared with cystic malignant tumors. The increased expression of all three mRNA species was mainly located in the stroma of poorly differentiated tumors and metastases. Apart from being expressed in the stroma of these tumors, uPAR mRNA was also expressed by tumor cells located along the stromal/epithelial boarder. In addition, the tumor tissue content of uPA, uPAR and PAI-1 mRNAs as measured by Northern blots were higher in the solid as compared with the cystic tumors. Increased expression of uPA, uPAR and PAI-1 genes in the solid tumors suggest a correlation with a more aggressive phenotype.},
  author       = {Borgfeldt, Christer and Hansson, Stefan and Gustavsson, Barbro and Måsbäck, Anna and Casslén, Bertil},
  issn         = {0020-7136},
  keyword      = {human,ovarian neoplasm,mRNA analysis,urokinase,plasminogen activation},
  language     = {eng},
  number       = {4},
  pages        = {497--502},
  publisher    = {John Wiley & Sons},
  series       = {International Journal of Cancer},
  title        = {Dedifferentiation of serous ovarian cancer from cystic to solid tumors is associated with increased expression of mRNA for urokinase plasminogen activator (uPA), its receptor (uPAR) and its inhibitor (PAI-1)},
  url          = {http://dx.doi.org/10.1002/ijc.1215},
  volume       = {92},
  year         = {2001},
}