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Endocardial expression and functional characterization of endothelin-1

Saetrum Opgaard, Ole; Adner, Mikael LU ; Peters, Theodorus H.F.; Xu, Cang-Bao LU ; Stavenow, Lars; Gulbenkian, Sergio; Erlinge, David LU ; Edvinsson, Lars LU and Sharma, Hari S. (2001) In Molecular and Cellular Biochemistry 224(1-2). p.151-158
Abstract
Endothelin-1 (ET-1), a 21 amino acid peptide exerts a wide range of biological activities including vasoconstriction, mitogenesis and inotropic effects on the heart. In this study, we examined whether endocardial endothelial cells express ET-1 and evaluated its functional properties. Using immunofluorescence localization method, we demonstrated cytoplasmic staining of ET-1 in the human endocardial endothelial cells from the right atrium and left ventricle. Employing reverse transcriptase polymerase chain reaction (RT-PCR) expression of ET-1 mRNA and its receptors ET(A) and ET(B) mRNAs were found in human myocardial as well as in endocardial endothelial cells. Biological activity of endocardial endothelial cells derived ET-1 was established... (More)
Endothelin-1 (ET-1), a 21 amino acid peptide exerts a wide range of biological activities including vasoconstriction, mitogenesis and inotropic effects on the heart. In this study, we examined whether endocardial endothelial cells express ET-1 and evaluated its functional properties. Using immunofluorescence localization method, we demonstrated cytoplasmic staining of ET-1 in the human endocardial endothelial cells from the right atrium and left ventricle. Employing reverse transcriptase polymerase chain reaction (RT-PCR) expression of ET-1 mRNA and its receptors ET(A) and ET(B) mRNAs were found in human myocardial as well as in endocardial endothelial cells. Biological activity of endocardial endothelial cells derived ET-1 was established as the conditioned media obtained from cultured porcine endocardial endothelial cells induced a slowly developing, strong and long-lasting contraction of circular rat aortic segments, with similar characteristics to that obtained with exogenous ET-1. Furthermore, the selective endothelin-A receptor antagonist, FR 139317, blocked the conditioned media induced contractions. Our results suggest that endocardial endothelial cells express and release biologically active ET-1 which could play a pivotal role in the regulation of myocardial contractility as well as a circulatory peptide may further act in other peripheral target organs. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
endothelin1, endocardial endothelial cells, immunofluorescence, in vitro pharmacology, FR139317
in
Molecular and Cellular Biochemistry
volume
224
issue
1-2
pages
151 - 158
publisher
Springer
external identifiers
  • pmid:11693192
  • scopus:0034800119
ISSN
0300-8177
DOI
10.1023/A:1011952504093
language
English
LU publication?
yes
id
2838ae92-45d8-42b0-a7b3-723d8fdf050a (old id 1121355)
date added to LUP
2008-07-09 09:32:50
date last changed
2018-05-29 11:34:40
@article{2838ae92-45d8-42b0-a7b3-723d8fdf050a,
  abstract     = {Endothelin-1 (ET-1), a 21 amino acid peptide exerts a wide range of biological activities including vasoconstriction, mitogenesis and inotropic effects on the heart. In this study, we examined whether endocardial endothelial cells express ET-1 and evaluated its functional properties. Using immunofluorescence localization method, we demonstrated cytoplasmic staining of ET-1 in the human endocardial endothelial cells from the right atrium and left ventricle. Employing reverse transcriptase polymerase chain reaction (RT-PCR) expression of ET-1 mRNA and its receptors ET(A) and ET(B) mRNAs were found in human myocardial as well as in endocardial endothelial cells. Biological activity of endocardial endothelial cells derived ET-1 was established as the conditioned media obtained from cultured porcine endocardial endothelial cells induced a slowly developing, strong and long-lasting contraction of circular rat aortic segments, with similar characteristics to that obtained with exogenous ET-1. Furthermore, the selective endothelin-A receptor antagonist, FR 139317, blocked the conditioned media induced contractions. Our results suggest that endocardial endothelial cells express and release biologically active ET-1 which could play a pivotal role in the regulation of myocardial contractility as well as a circulatory peptide may further act in other peripheral target organs.},
  author       = {Saetrum Opgaard, Ole and Adner, Mikael and Peters, Theodorus H.F. and Xu, Cang-Bao and Stavenow, Lars and Gulbenkian, Sergio and Erlinge, David and Edvinsson, Lars and Sharma, Hari S.},
  issn         = {0300-8177},
  keyword      = {endothelin1,endocardial endothelial cells,immunofluorescence,in vitro pharmacology,FR139317},
  language     = {eng},
  number       = {1-2},
  pages        = {151--158},
  publisher    = {Springer},
  series       = {Molecular and Cellular Biochemistry},
  title        = {Endocardial expression and functional characterization of endothelin-1},
  url          = {http://dx.doi.org/10.1023/A:1011952504093},
  volume       = {224},
  year         = {2001},
}