N-methyl-D-aspartate receptor-mediated increase of neurogenesis in adult rat dentate gyrus following stroke
Arvidsson, Andreas LU ; Kokaia, Zaal LU
and Lindvall, Olle
LU
(2001)
In European Journal of Neuroscience
14(1).
p.10-18
- Abstract
- Neurogenesis in the adult rat dentate gyrus was studied following focal ischemic insults produced by middle cerebral artery occlusion (MCAO). Animals were subjected to either 30 min of MCAO, which causes damage confined to the striatum, or 2 h of MCAO, which leads to both striatal and cortical infarction. When compared to sham-operated rats, MCAO-rats showed a marked increase of the number of cells double-labelled for 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU; injected during 4-6 days postischemia) and neuronal-specific antigen (NeuN; a marker of postmitotic neurons) in the ipsilateral dentate granule cell layer and subgranular zone at 5 weeks following the 2 h insult. Only a modest and variable increase of BrdU-labelled cells was... (More)
- Neurogenesis in the adult rat dentate gyrus was studied following focal ischemic insults produced by middle cerebral artery occlusion (MCAO). Animals were subjected to either 30 min of MCAO, which causes damage confined to the striatum, or 2 h of MCAO, which leads to both striatal and cortical infarction. When compared to sham-operated rats, MCAO-rats showed a marked increase of the number of cells double-labelled for 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU; injected during 4-6 days postischemia) and neuronal-specific antigen (NeuN; a marker of postmitotic neurons) in the ipsilateral dentate granule cell layer and subgranular zone at 5 weeks following the 2 h insult. Only a modest and variable increase of BrdU-labelled cells was found after 30 min of MCAO. The enhanced neurogenesis was not dependent on cell death in the hippocampus, and its magnitude was not correlated to the degree of cortical damage. Systemic administration of the N-methyl-D-aspartate (NMDA) receptor blocker dizocilpine maleate (MK-801) completely suppressed the elevated neurogenesis following 2 h of MCAO. Our findings indicate that stroke leads to increased neurogenesis in the adult rat dentate gyrus through glutamatergic mechanisms acting on NMDA receptors. This modulatory effect may be mediated through changes in the levels of several growth factors, which occur after stroke, and could influence various regulatory steps of neurogenesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1121992
- author
- Arvidsson, Andreas
LU
; Kokaia, Zaal
LU
and Lindvall, Olle
LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Neuroscience
- volume
- 14
- issue
- 1
- pages
- 10 - 18
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:11488944
- scopus:0034909799
- ISSN
- 1460-9568
- DOI
- 10.1046/j.0953-816x.2001.01611.x
- language
- English
- LU publication?
- yes
- id
- f9a74612-d5e0-4cf9-9529-446a8f0313a9 (old id 1121992)
- date added to LUP
- 2016-04-01 12:12:28
- date last changed
- 2022-01-27 00:27:22
@article{f9a74612-d5e0-4cf9-9529-446a8f0313a9,
abstract = {{Neurogenesis in the adult rat dentate gyrus was studied following focal ischemic insults produced by middle cerebral artery occlusion (MCAO). Animals were subjected to either 30 min of MCAO, which causes damage confined to the striatum, or 2 h of MCAO, which leads to both striatal and cortical infarction. When compared to sham-operated rats, MCAO-rats showed a marked increase of the number of cells double-labelled for 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU; injected during 4-6 days postischemia) and neuronal-specific antigen (NeuN; a marker of postmitotic neurons) in the ipsilateral dentate granule cell layer and subgranular zone at 5 weeks following the 2 h insult. Only a modest and variable increase of BrdU-labelled cells was found after 30 min of MCAO. The enhanced neurogenesis was not dependent on cell death in the hippocampus, and its magnitude was not correlated to the degree of cortical damage. Systemic administration of the N-methyl-D-aspartate (NMDA) receptor blocker dizocilpine maleate (MK-801) completely suppressed the elevated neurogenesis following 2 h of MCAO. Our findings indicate that stroke leads to increased neurogenesis in the adult rat dentate gyrus through glutamatergic mechanisms acting on NMDA receptors. This modulatory effect may be mediated through changes in the levels of several growth factors, which occur after stroke, and could influence various regulatory steps of neurogenesis.}},
author = {{Arvidsson, Andreas and Kokaia, Zaal and Lindvall, Olle}},
issn = {{1460-9568}},
language = {{eng}},
number = {{1}},
pages = {{10--18}},
publisher = {{Wiley-Blackwell}},
series = {{European Journal of Neuroscience}},
title = {{N-methyl-D-aspartate receptor-mediated increase of neurogenesis in adult rat dentate gyrus following stroke}},
url = {{http://dx.doi.org/10.1046/j.0953-816x.2001.01611.x}},
doi = {{10.1046/j.0953-816x.2001.01611.x}},
volume = {{14}},
year = {{2001}},
}