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Identification of the translocating bacteria in rats with acute liver injury and their relation to the bacterial flora of the intestinal mucosa

Wang, Mei LU ; Adawi, Diya LU ; Molin, Göran LU ; Pettersson, Bertil; Jeppsson, Bengt LU and Ahrné, Siv LU (2001) In APMIS : acta pathologica, microbiologica, et immunologica Scandinavica1988-01-01+01:00 109(7-8). p.551-558
Abstract
The bacterial flora of the intestine and the bacteria found in liver, mesenteric lymph nodes, portal and arterial blood after D-galactosamine-induced liver injury, with and without pretreatment with Lactobacillus plantarum DSM 9843, were studied in the rat. Dominating representatives were identified to species level by 16S rDNA sequencing and typed by randomly amplified polymorphic DNA (RAPD) and by restriction endonuclease analysis (REA) for strain definition. It was proven that bacterial strains from the intestine occur at extraintestinal sites after liver injury. Lactobacillus spp. dominated the intestinal flora and were also the most frequently found genus in the liver and the mesenteric lymph nodes. Some of the blood isolates,... (More)
The bacterial flora of the intestine and the bacteria found in liver, mesenteric lymph nodes, portal and arterial blood after D-galactosamine-induced liver injury, with and without pretreatment with Lactobacillus plantarum DSM 9843, were studied in the rat. Dominating representatives were identified to species level by 16S rDNA sequencing and typed by randomly amplified polymorphic DNA (RAPD) and by restriction endonuclease analysis (REA) for strain definition. It was proven that bacterial strains from the intestine occur at extraintestinal sites after liver injury. Lactobacillus spp. dominated the intestinal flora and were also the most frequently found genus in the liver and the mesenteric lymph nodes. Some of the blood isolates, identified as Staphylococcus aureus, Proteus vulgaris and Bacteroides merdae, were not found as a dominating part of the mucosal flora. Treatment with L. plantarum before liver injury decreased translocation and made the intestinal flora increasingly dominated by lactobacilli. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Bacterial translocation, rat intestinal flora, probiotics, Lactobacillus plantarum, acute liver injury
in
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica1988-01-01+01:00
volume
109
issue
7-8
pages
551 - 558
publisher
John Wiley & Sons
external identifiers
  • pmid:11552954
  • scopus:0034849841
ISSN
1600-0463
DOI
10.1111/j.1600-0463.2001.907810.x
language
English
LU publication?
yes
id
b0a57ea7-772c-4fa2-a606-18a4afe16bcb (old id 1122498)
date added to LUP
2008-07-17 11:06:34
date last changed
2018-01-07 05:32:21
@article{b0a57ea7-772c-4fa2-a606-18a4afe16bcb,
  abstract     = {The bacterial flora of the intestine and the bacteria found in liver, mesenteric lymph nodes, portal and arterial blood after D-galactosamine-induced liver injury, with and without pretreatment with Lactobacillus plantarum DSM 9843, were studied in the rat. Dominating representatives were identified to species level by 16S rDNA sequencing and typed by randomly amplified polymorphic DNA (RAPD) and by restriction endonuclease analysis (REA) for strain definition. It was proven that bacterial strains from the intestine occur at extraintestinal sites after liver injury. Lactobacillus spp. dominated the intestinal flora and were also the most frequently found genus in the liver and the mesenteric lymph nodes. Some of the blood isolates, identified as Staphylococcus aureus, Proteus vulgaris and Bacteroides merdae, were not found as a dominating part of the mucosal flora. Treatment with L. plantarum before liver injury decreased translocation and made the intestinal flora increasingly dominated by lactobacilli.},
  author       = {Wang, Mei and Adawi, Diya and Molin, Göran and Pettersson, Bertil and Jeppsson, Bengt and Ahrné, Siv},
  issn         = {1600-0463},
  keyword      = {Bacterial translocation,rat intestinal flora,probiotics,Lactobacillus plantarum,acute liver injury},
  language     = {eng},
  number       = {7-8},
  pages        = {551--558},
  publisher    = {John Wiley & Sons},
  series       = { APMIS : acta pathologica, microbiologica, et immunologica Scandinavica1988-01-01+01:00},
  title        = {Identification of the translocating bacteria in rats with acute liver injury and their relation to the bacterial flora of the intestinal mucosa},
  url          = {http://dx.doi.org/10.1111/j.1600-0463.2001.907810.x},
  volume       = {109},
  year         = {2001},
}