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Hypothalamic gene expression following ghrelin therapy to gastrectomized rodents

Egecioglu, Emil; Stenstrom, Bjorn; Pinnock, Scarlett B; Tung, Loraine Y C; de la Cour, Charlotta LU ; Lindqvist, Andreas LU ; Håkanson, Rolf LU ; Syversen, Unni; Chen, Duan and Dickson, Suzanne L (2008) In Regulatory Peptides 146(1-3). p.176-182
Abstract
We investigated whether ghrelin depletion (by gastrectomy surgery) and/or treatment/replacement with the gastric hormone ghrelin alters the expression of key hypothalamic genes involved in energy balance, in a manner consistent with ghrelin's pro-obesity effects. At 2 weeks after surgery mice were treated with ghrelin (12 nmol/mouse/day, sc) or vehicle for 8 weeks. Gastrectomy had little effect on the expression of these genes, with the exception of NPY mRNA in the arcuate nucleus that was increased. Ghrelin treatment (to gastrectomized and sham mice) increased the mRNA expression of orexigenic peptides NPY and AgRP while decreasing mRNA expression of the anorexigenic peptide POMC. Two weeks gavage treatment with the ghrelin mimetic,... (More)
We investigated whether ghrelin depletion (by gastrectomy surgery) and/or treatment/replacement with the gastric hormone ghrelin alters the expression of key hypothalamic genes involved in energy balance, in a manner consistent with ghrelin's pro-obesity effects. At 2 weeks after surgery mice were treated with ghrelin (12 nmol/mouse/day, sc) or vehicle for 8 weeks. Gastrectomy had little effect on the expression of these genes, with the exception of NPY mRNA in the arcuate nucleus that was increased. Ghrelin treatment (to gastrectomized and sham mice) increased the mRNA expression of orexigenic peptides NPY and AgRP while decreasing mRNA expression of the anorexigenic peptide POMC. Two weeks gavage treatment with the ghrelin mimetic, MK-0677, to rats increased NPY and POMC mRNA in the arcuate nucleus and MCH mRNA in the lateral hypothalamus. Thus, while predicted pro-obesity ghrelin signalling pathways were activated by ghrelin and ghrelin mimetics, these were largely unaffected by gastrectomy. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Regulatory Peptides
volume
146
issue
1-3
pages
176 - 182
publisher
Elsevier
external identifiers
  • pmid:17936372
  • wos:000253278600024
  • scopus:38149059686
ISSN
1873-1686
DOI
10.1016/j.regpep.2007.09.006
language
English
LU publication?
yes
id
733ee043-a648-40eb-8996-642a96c1bdc7 (old id 1139102)
date added to LUP
2008-08-05 15:48:38
date last changed
2017-10-08 03:29:11
@article{733ee043-a648-40eb-8996-642a96c1bdc7,
  abstract     = {We investigated whether ghrelin depletion (by gastrectomy surgery) and/or treatment/replacement with the gastric hormone ghrelin alters the expression of key hypothalamic genes involved in energy balance, in a manner consistent with ghrelin's pro-obesity effects. At 2 weeks after surgery mice were treated with ghrelin (12 nmol/mouse/day, sc) or vehicle for 8 weeks. Gastrectomy had little effect on the expression of these genes, with the exception of NPY mRNA in the arcuate nucleus that was increased. Ghrelin treatment (to gastrectomized and sham mice) increased the mRNA expression of orexigenic peptides NPY and AgRP while decreasing mRNA expression of the anorexigenic peptide POMC. Two weeks gavage treatment with the ghrelin mimetic, MK-0677, to rats increased NPY and POMC mRNA in the arcuate nucleus and MCH mRNA in the lateral hypothalamus. Thus, while predicted pro-obesity ghrelin signalling pathways were activated by ghrelin and ghrelin mimetics, these were largely unaffected by gastrectomy.},
  author       = {Egecioglu, Emil and Stenstrom, Bjorn and Pinnock, Scarlett B and Tung, Loraine Y C and de la Cour, Charlotta and Lindqvist, Andreas and Håkanson, Rolf and Syversen, Unni and Chen, Duan and Dickson, Suzanne L},
  issn         = {1873-1686},
  language     = {eng},
  number       = {1-3},
  pages        = {176--182},
  publisher    = {Elsevier},
  series       = {Regulatory Peptides},
  title        = {Hypothalamic gene expression following ghrelin therapy to gastrectomized rodents},
  url          = {http://dx.doi.org/10.1016/j.regpep.2007.09.006},
  volume       = {146},
  year         = {2008},
}