Epitope Analysis of GAD65 Binding in both Cord Blood and at the Time of Clinical Diagnosis of Childhood Type 1 Diabetes.
Elfving, Maria LU ; Lindberg, Bengt LU ; Lynch, Kristian LU ; Ivarsson, Sten LU ; Lernmark, Åke LU
and Hampe, C. S.
(2007)
In Hormone and Metabolic Research
39(11).
p.790-796
- Abstract
- The GAD65 epitope immunoglobulin binding pattern in cord blood of children (n=37), who later developed type 1 diabetes at 3.2-14.9 years of age, was analyzed. First, the binding at diagnosis was compared with that in the cord blood serum. The next comparison was between the cord blood serum and the mothers' serum taken at delivery. Basal GAD65 binding levels were determined in Protein A Sepharose-based radio-binding assays with S-35-labeled human and rat GAD65, rat GAD67 and GAD65/67 fusion proteins representing N-terminal (N), middle (M) and C-terminal (C) epitopes. In the first comparison, 28/37 children had GAD65 binding above 2.44 relative units (RU) (upper three quartiles), representing a marked increase from birth in the binding to... (More)
- The GAD65 epitope immunoglobulin binding pattern in cord blood of children (n=37), who later developed type 1 diabetes at 3.2-14.9 years of age, was analyzed. First, the binding at diagnosis was compared with that in the cord blood serum. The next comparison was between the cord blood serum and the mothers' serum taken at delivery. Basal GAD65 binding levels were determined in Protein A Sepharose-based radio-binding assays with S-35-labeled human and rat GAD65, rat GAD67 and GAD65/67 fusion proteins representing N-terminal (N), middle (M) and C-terminal (C) epitopes. In the first comparison, 28/37 children had GAD65 binding above 2.44 relative units (RU) (upper three quartiles), representing a marked increase from birth in the binding to human GAD65 (p < 0.0001), rat GAD65 (p < 0.0001), N- (p = 0.04), M- (p < 0.0001), C- (p=0.001), and M + C-epitopes (p < 0.0001), but not to rat GAD67. At birth, 9/37 had GAD65 binding above 1.56 RU (upper quartile) demonstrating that their binding of human S-35-GAD65 was higher in cord blood than in the mother (p=0.008). Higher cord blood binding was also observed for the N- (p=0.02) terminal epitope but not for rat GAD65, rat GAD67, and the remaining epitopes. These data suggest that differences in the epitope GAD65 binding between mother and child at birth are limited. In contrast, the epitope pattern at diagnosis differed from that at birth, supporting the view that disease-associated epitopes develop between birth and diagnosis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1140883
- author
- Elfving, Maria
LU
; Lindberg, Bengt
LU
; Lynch, Kristian
LU
; Ivarsson, Sten
LU
; Lernmark, Åke
LU
and Hampe, C. S.
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cord blood islet autoantibodies, epitope analysis, diabetes, mellitus, glutamic acid decarboxylase, type 1 (insulin-dependent)
- in
- Hormone and Metabolic Research
- volume
- 39
- issue
- 11
- pages
- 790 - 796
- publisher
- Georg Thieme Verlag
- external identifiers
-
- scopus:36749078111
- wos:000251545700004
- pmid:17992632
- ISSN
- 1439-4286
- DOI
- 10.1055/s-2007-992128
- language
- English
- LU publication?
- yes
- id
- 199a5506-7104-48ba-8044-4ec1be4abc21 (old id 1140883)
- date added to LUP
- 2016-04-01 15:37:28
- date last changed
- 2022-01-28 06:16:33
@article{199a5506-7104-48ba-8044-4ec1be4abc21,
abstract = {{The GAD65 epitope immunoglobulin binding pattern in cord blood of children (n=37), who later developed type 1 diabetes at 3.2-14.9 years of age, was analyzed. First, the binding at diagnosis was compared with that in the cord blood serum. The next comparison was between the cord blood serum and the mothers' serum taken at delivery. Basal GAD65 binding levels were determined in Protein A Sepharose-based radio-binding assays with S-35-labeled human and rat GAD65, rat GAD67 and GAD65/67 fusion proteins representing N-terminal (N), middle (M) and C-terminal (C) epitopes. In the first comparison, 28/37 children had GAD65 binding above 2.44 relative units (RU) (upper three quartiles), representing a marked increase from birth in the binding to human GAD65 (p < 0.0001), rat GAD65 (p < 0.0001), N- (p = 0.04), M- (p < 0.0001), C- (p=0.001), and M + C-epitopes (p < 0.0001), but not to rat GAD67. At birth, 9/37 had GAD65 binding above 1.56 RU (upper quartile) demonstrating that their binding of human S-35-GAD65 was higher in cord blood than in the mother (p=0.008). Higher cord blood binding was also observed for the N- (p=0.02) terminal epitope but not for rat GAD65, rat GAD67, and the remaining epitopes. These data suggest that differences in the epitope GAD65 binding between mother and child at birth are limited. In contrast, the epitope pattern at diagnosis differed from that at birth, supporting the view that disease-associated epitopes develop between birth and diagnosis.}},
author = {{Elfving, Maria and Lindberg, Bengt and Lynch, Kristian and Ivarsson, Sten and Lernmark, Åke and Hampe, C. S.}},
issn = {{1439-4286}},
keywords = {{cord blood islet autoantibodies; epitope analysis; diabetes; mellitus; glutamic acid decarboxylase; type 1 (insulin-dependent)}},
language = {{eng}},
number = {{11}},
pages = {{790--796}},
publisher = {{Georg Thieme Verlag}},
series = {{Hormone and Metabolic Research}},
title = {{Epitope Analysis of GAD65 Binding in both Cord Blood and at the Time of Clinical Diagnosis of Childhood Type 1 Diabetes.}},
url = {{http://dx.doi.org/10.1055/s-2007-992128}},
doi = {{10.1055/s-2007-992128}},
volume = {{39}},
year = {{2007}},
}