Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Elastin- and Collagen-Rich Human Carotid Plaques Have Increased Levels of the Cysteine Protease Inhibitor Cystatin C.

Goncalves, Isabel LU orcid ; Ares, Mikko P S ; Moberg, Anna ; Moses, Jonatan LU ; To, Fong LU ; Montan, Jonathan LU ; Pedro, Luís M ; Dias, Nuno LU orcid ; Fernandes E Fernandes, José and Nordin Fredrikson, Gunilla LU , et al. (2008) In Journal of Vascular Research 45(5). p.395-401
Abstract
Background: Cystatin C is a major inhibitor of the elastin- and collagen-degrading cysteine proteases and may therefore have an important role in preserving atherosclerotic plaque stability. In this study we analyzed the associations between human carotid plaque cystatin C expression and the plaque content of collagen and elastin. Methods: Thirty-one plaques were removed by endarterectomy and homogenized. Cystatin C levels were analyzed by densitometry of Western blots and elastin and collagen levels were determined colorimetrically. Results: The plaque content of cystatin C correlated with total elastin (r = 0.58, p = 0.001) and collagen (r = 0.50, p = 0.004), as well as with cross-linked forms of elastin (r = 0.42, p = 0.022) and... (More)
Background: Cystatin C is a major inhibitor of the elastin- and collagen-degrading cysteine proteases and may therefore have an important role in preserving atherosclerotic plaque stability. In this study we analyzed the associations between human carotid plaque cystatin C expression and the plaque content of collagen and elastin. Methods: Thirty-one plaques were removed by endarterectomy and homogenized. Cystatin C levels were analyzed by densitometry of Western blots and elastin and collagen levels were determined colorimetrically. Results: The plaque content of cystatin C correlated with total elastin (r = 0.58, p = 0.001) and collagen (r = 0.50, p = 0.004), as well as with cross-linked forms of elastin (r = 0.42, p = 0.022) and collagen (r = 0.52, p = 0.003). Immunohistochemical analysis demonstrated that cystatin C colocalized with elastin and collagen. No correlation was seen between cystatin C and the amount of degraded elastin or collagen in plaques. Conclusion: The positive correlation between cystatin C levels and collagen and elastin levels in plaques supports the notion that cystatin C plays an important role in maintaining atherosclerotic plaque stability. Copyright (c) 2008 S. Karger AG, Basel. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Vascular Research
volume
45
issue
5
pages
395 - 401
publisher
Karger
external identifiers
  • wos:000258318300004
  • pmid:18376131
  • scopus:50349083364
  • pmid:18376131
ISSN
1423-0135
DOI
10.1159/000121474
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012), Unit for Clinical Vascular Disease Research (013242410), Experimental Cardiovascular Research Unit (013242110)
id
44379c97-c874-4795-858e-764af0bbd20a (old id 1147944)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18376131?dopt=Abstract
date added to LUP
2016-04-04 09:29:28
date last changed
2022-08-01 03:33:20
@article{44379c97-c874-4795-858e-764af0bbd20a,
  abstract     = {{Background: Cystatin C is a major inhibitor of the elastin- and collagen-degrading cysteine proteases and may therefore have an important role in preserving atherosclerotic plaque stability. In this study we analyzed the associations between human carotid plaque cystatin C expression and the plaque content of collagen and elastin. Methods: Thirty-one plaques were removed by endarterectomy and homogenized. Cystatin C levels were analyzed by densitometry of Western blots and elastin and collagen levels were determined colorimetrically. Results: The plaque content of cystatin C correlated with total elastin (r = 0.58, p = 0.001) and collagen (r = 0.50, p = 0.004), as well as with cross-linked forms of elastin (r = 0.42, p = 0.022) and collagen (r = 0.52, p = 0.003). Immunohistochemical analysis demonstrated that cystatin C colocalized with elastin and collagen. No correlation was seen between cystatin C and the amount of degraded elastin or collagen in plaques. Conclusion: The positive correlation between cystatin C levels and collagen and elastin levels in plaques supports the notion that cystatin C plays an important role in maintaining atherosclerotic plaque stability. Copyright (c) 2008 S. Karger AG, Basel.}},
  author       = {{Goncalves, Isabel and Ares, Mikko P S and Moberg, Anna and Moses, Jonatan and To, Fong and Montan, Jonathan and Pedro, Luís M and Dias, Nuno and Fernandes E Fernandes, José and Nordin Fredrikson, Gunilla and Nilsson, Jan and Jovinge, Stefan and Bengtsson, Eva}},
  issn         = {{1423-0135}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{395--401}},
  publisher    = {{Karger}},
  series       = {{Journal of Vascular Research}},
  title        = {{Elastin- and Collagen-Rich Human Carotid Plaques Have Increased Levels of the Cysteine Protease Inhibitor Cystatin C.}},
  url          = {{http://dx.doi.org/10.1159/000121474}},
  doi          = {{10.1159/000121474}},
  volume       = {{45}},
  year         = {{2008}},
}