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PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes.

Mootha, VK; Lindgren, Cecilia LU ; Eriksson, Karl-Fredrik LU ; Subramanian, A; Sihag, S; Lehar, J; Puigserver, P; Nilsson, Emma A LU ; Ridderstråle, Martin LU and Laurila, Esa LU , et al. (2003) In Nature Genetics 34(3). p.267-273
Abstract
DNA microarrays can be used to identify gene expression changes characteristic of human disease. This is challenging, however, when relevant differences are subtle at the level of individual genes. We introduce an analytical strategy, Gene Set Enrichment Analysis, designed to detect modest but coordinate changes in the expression of groups of functionally related genes. Using this approach, we identify a set of genes involved in oxidative phosphorylation whose expression is coordinately decreased in human diabetic muscle. Expression of these genes is high at sites of insulin-mediated glucose disposal, activated by PGC-1alpha and correlated with total-body aerobic capacity. Our results associate this gene set with clinically important... (More)
DNA microarrays can be used to identify gene expression changes characteristic of human disease. This is challenging, however, when relevant differences are subtle at the level of individual genes. We introduce an analytical strategy, Gene Set Enrichment Analysis, designed to detect modest but coordinate changes in the expression of groups of functionally related genes. Using this approach, we identify a set of genes involved in oxidative phosphorylation whose expression is coordinately decreased in human diabetic muscle. Expression of these genes is high at sites of insulin-mediated glucose disposal, activated by PGC-1alpha and correlated with total-body aerobic capacity. Our results associate this gene set with clinically important variation in human metabolism and illustrate the value of pathway relationships in the analysis of genomic profiling experiments. (Less)
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Nature Genetics
volume
34
issue
3
pages
267 - 273
publisher
Nature Publishing Group
external identifiers
  • pmid:12808457
  • wos:000183815300013
  • scopus:0038054341
ISSN
1546-1718
DOI
language
English
LU publication?
yes
id
7bb5f6c1-6141-4fe9-bcb4-b3b05f89ebaf (old id 117054)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12808457&dopt=Abstract
date added to LUP
2007-07-26 14:24:15
date last changed
2018-06-17 04:45:34
@article{7bb5f6c1-6141-4fe9-bcb4-b3b05f89ebaf,
  abstract     = {DNA microarrays can be used to identify gene expression changes characteristic of human disease. This is challenging, however, when relevant differences are subtle at the level of individual genes. We introduce an analytical strategy, Gene Set Enrichment Analysis, designed to detect modest but coordinate changes in the expression of groups of functionally related genes. Using this approach, we identify a set of genes involved in oxidative phosphorylation whose expression is coordinately decreased in human diabetic muscle. Expression of these genes is high at sites of insulin-mediated glucose disposal, activated by PGC-1alpha and correlated with total-body aerobic capacity. Our results associate this gene set with clinically important variation in human metabolism and illustrate the value of pathway relationships in the analysis of genomic profiling experiments.},
  author       = {Mootha, VK and Lindgren, Cecilia and Eriksson, Karl-Fredrik and Subramanian, A and Sihag, S and Lehar, J and Puigserver, P and Nilsson, Emma A and Ridderstråle, Martin and Laurila, Esa and Houstis, N and Daly, MJ and Patterson, N and Mesirov, JP and Golub, TR and Tamayo, P and Spiegelman, B and Lander, ES and Hirschhorn, JN and Altshuler, D and Groop, Leif},
  issn         = {1546-1718},
  language     = {eng},
  number       = {3},
  pages        = {267--273},
  publisher    = {Nature Publishing Group},
  series       = {Nature Genetics},
  title        = {PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes.},
  url          = {http://dx.doi.org/},
  volume       = {34},
  year         = {2003},
}