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Enteral crude red kidney bean (Phaseolus vulgaris) lectin--phytohemagglutinin--induces maturational changes in the enterocyte membrane proteins of suckling rats.

Kruszewska, Danuta LU ; Kiela, Pawel; Ljungh, Åsa LU ; Erlwanger, Kennedy H; Weström, Björn LU ; Linderoth, Ann LU and Pierzynowski, Stefan LU (2003) In Biology of the Neonate 84(2). p.152-158
Abstract
This study aimed to investigate the effect of enterally administered crude red kidney bean (Phaseolus vulgaris) lectin, PHA, on the expression of brush-border membrane vesicle (BBMV) proteins, in particular Na+/H+ exchangers (NHEs), in the small intestine of suckling rats. Gavage of PHA to 14-day-old rats for 3 days resulted in altered protein/glycoprotein patterns as analyzed by SDS-PAGE. Immunoblots demonstrated the appearance of two 71- and 27-kD protein bands indicative for NHE3 - one of the NHE isoforms - and PHA, respectively. PHA treatment also resulted in an augmented uptake of 22Na+ by the BBMV indicating an increase in NHE activity. Overall, the data suggests that enteral PHA exposure may induce maturational changes in enterocyte... (More)
This study aimed to investigate the effect of enterally administered crude red kidney bean (Phaseolus vulgaris) lectin, PHA, on the expression of brush-border membrane vesicle (BBMV) proteins, in particular Na+/H+ exchangers (NHEs), in the small intestine of suckling rats. Gavage of PHA to 14-day-old rats for 3 days resulted in altered protein/glycoprotein patterns as analyzed by SDS-PAGE. Immunoblots demonstrated the appearance of two 71- and 27-kD protein bands indicative for NHE3 - one of the NHE isoforms - and PHA, respectively. PHA treatment also resulted in an augmented uptake of 22Na+ by the BBMV indicating an increase in NHE activity. Overall, the data suggests that enteral PHA exposure may induce maturational changes in enterocyte membrane proteins in young rats. In view of these findings, an investigation into the addition of PHA to infant formulas and weaning diets is warranted. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biology of the Neonate
volume
84
issue
2
pages
152 - 158
publisher
Karger
external identifiers
  • pmid:12907850
  • wos:000184873000007
  • scopus:0042161697
ISSN
1421-9727
DOI
10.1159/000071950
language
English
LU publication?
yes
id
59e7257f-ef46-444a-a53f-4b27bba23add (old id 117443)
date added to LUP
2007-07-10 07:31:13
date last changed
2018-05-29 11:43:54
@article{59e7257f-ef46-444a-a53f-4b27bba23add,
  abstract     = {This study aimed to investigate the effect of enterally administered crude red kidney bean (Phaseolus vulgaris) lectin, PHA, on the expression of brush-border membrane vesicle (BBMV) proteins, in particular Na+/H+ exchangers (NHEs), in the small intestine of suckling rats. Gavage of PHA to 14-day-old rats for 3 days resulted in altered protein/glycoprotein patterns as analyzed by SDS-PAGE. Immunoblots demonstrated the appearance of two 71- and 27-kD protein bands indicative for NHE3 - one of the NHE isoforms - and PHA, respectively. PHA treatment also resulted in an augmented uptake of 22Na+ by the BBMV indicating an increase in NHE activity. Overall, the data suggests that enteral PHA exposure may induce maturational changes in enterocyte membrane proteins in young rats. In view of these findings, an investigation into the addition of PHA to infant formulas and weaning diets is warranted.},
  author       = {Kruszewska, Danuta and Kiela, Pawel and Ljungh, Åsa and Erlwanger, Kennedy H and Weström, Björn and Linderoth, Ann and Pierzynowski, Stefan},
  issn         = {1421-9727},
  language     = {eng},
  number       = {2},
  pages        = {152--158},
  publisher    = {Karger},
  series       = {Biology of the Neonate},
  title        = {Enteral crude red kidney bean (Phaseolus vulgaris) lectin--phytohemagglutinin--induces maturational changes in the enterocyte membrane proteins of suckling rats.},
  url          = {http://dx.doi.org/10.1159/000071950},
  volume       = {84},
  year         = {2003},
}