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Reduced enterotoxin D formation on boiled ham in staphylococcus aureus Δagr mutant

Susilo, Yusak Budi LU ; Sihto, Henna Maria; Rådström, Peter LU ; Stephan, Roger; Johler, Sophia and Schelin, Jenny LU (2017) In Toxins 9(9).
Abstract

Staphylococcal food poisoning (SFP) is a common cause of foodborne illness worldwide, and enterotoxin D (SED) is one of the most frequent Staphylococcus aureus enterotoxins associated with it. It has been reported that the expression and formation of SED in S. aureus is regulated by the quorum sensing Agr system. In this study, the effect of agr deletion on sed expression in S. aureus grown on boiled ham was investigated. Growth, sed mRNA and SED protein levels in an S. aureus wild type strain and its isogenic Δagr mutant were monitored for 14 days at 22 °C. The results showed that although deletion of the agr gene did not affect the growth rate or maximum cell density of S. aureus on boiled ham, it had a pronounced effect on SED... (More)

Staphylococcal food poisoning (SFP) is a common cause of foodborne illness worldwide, and enterotoxin D (SED) is one of the most frequent Staphylococcus aureus enterotoxins associated with it. It has been reported that the expression and formation of SED in S. aureus is regulated by the quorum sensing Agr system. In this study, the effect of agr deletion on sed expression in S. aureus grown on boiled ham was investigated. Growth, sed mRNA and SED protein levels in an S. aureus wild type strain and its isogenic Δagr mutant were monitored for 14 days at 22 °C. The results showed that although deletion of the agr gene did not affect the growth rate or maximum cell density of S. aureus on boiled ham, it had a pronounced effect on SED formation during the first 5 days of incubation. The SED concentration was not reflected in the amount of preceding sed transcripts, suggesting that sed transcription levels may not always reflect SED formation. The expression of RNAIII transcript, the regulatory signal of the Agr system, was also monitored. Similar transcription patterns were observed for RNAIII and sed. Surprisingly, in the Δagr mutant, sed expression was comparable to that in the wild type strain, and was thus unaffected by deletion of the Agr system. These results demonstrate that the Agr system appears to only partially affect SED formation, even in a real food environment.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Agr quorum sensing system, Boiled ham, RNAIII, SED formation, Staphylococcal food poisoning, Staphylococcus aureus
in
Toxins
volume
9
issue
9
publisher
MDPI AG
external identifiers
  • scopus:85028622790
  • wos:000411961700009
ISSN
2072-6651
DOI
10.3390/toxins9090263
language
English
LU publication?
yes
id
11770b62-1755-47ce-9011-3aad17d313b7
date added to LUP
2017-09-26 12:00:01
date last changed
2018-01-16 13:26:45
@article{11770b62-1755-47ce-9011-3aad17d313b7,
  abstract     = {<p>Staphylococcal food poisoning (SFP) is a common cause of foodborne illness worldwide, and enterotoxin D (SED) is one of the most frequent Staphylococcus aureus enterotoxins associated with it. It has been reported that the expression and formation of SED in S. aureus is regulated by the quorum sensing Agr system. In this study, the effect of agr deletion on sed expression in S. aureus grown on boiled ham was investigated. Growth, sed mRNA and SED protein levels in an S. aureus wild type strain and its isogenic Δagr mutant were monitored for 14 days at 22 °C. The results showed that although deletion of the agr gene did not affect the growth rate or maximum cell density of S. aureus on boiled ham, it had a pronounced effect on SED formation during the first 5 days of incubation. The SED concentration was not reflected in the amount of preceding sed transcripts, suggesting that sed transcription levels may not always reflect SED formation. The expression of RNAIII transcript, the regulatory signal of the Agr system, was also monitored. Similar transcription patterns were observed for RNAIII and sed. Surprisingly, in the Δagr mutant, sed expression was comparable to that in the wild type strain, and was thus unaffected by deletion of the Agr system. These results demonstrate that the Agr system appears to only partially affect SED formation, even in a real food environment.</p>},
  articleno    = {263},
  author       = {Susilo, Yusak Budi and Sihto, Henna Maria and Rådström, Peter and Stephan, Roger and Johler, Sophia and Schelin, Jenny},
  issn         = {2072-6651},
  keyword      = {Agr quorum sensing system,Boiled ham,RNAIII,SED formation,Staphylococcal food poisoning,Staphylococcus aureus},
  language     = {eng},
  month        = {09},
  number       = {9},
  publisher    = {MDPI AG},
  series       = {Toxins},
  title        = {Reduced enterotoxin D formation on boiled ham in staphylococcus aureus Δagr mutant},
  url          = {http://dx.doi.org/10.3390/toxins9090263},
  volume       = {9},
  year         = {2017},
}