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Pax6 promotes neurogenesis in human neural stem cells.

Kallur, Therese LU ; Gisler, Ramiro LU ; Lindvall, Olle LU and Kokaia, Zaal LU (2008) In Molecular and Cellular Neuroscience 38(4). p.616-628
Abstract
During brain embryogenesis, transcription factors drive stem cells towards neuronal fate. Here we show that the transcription factor Pax6 increased in vitro generation of neurons from striatal but not cortical neural stem cells (NSCs), derived from 6 to 9 weeks old human fetuses, without affecting survival and proliferation. Overexpression of mouse Pax6 produced increased numbers of GABA+ and DARPP-32+ (characteristic of striatum) but not glutamate+ neurons (characteristic of cortex). Pax6-overexpressing cells survived and migrated to the same extent as control cells at 1 month after intrastriatal transplantation into newborn rats and generated more neuroblasts. Overexpression of mouse Pax6 in human NSCs also leads to altered levels of... (More)
During brain embryogenesis, transcription factors drive stem cells towards neuronal fate. Here we show that the transcription factor Pax6 increased in vitro generation of neurons from striatal but not cortical neural stem cells (NSCs), derived from 6 to 9 weeks old human fetuses, without affecting survival and proliferation. Overexpression of mouse Pax6 produced increased numbers of GABA+ and DARPP-32+ (characteristic of striatum) but not glutamate+ neurons (characteristic of cortex). Pax6-overexpressing cells survived and migrated to the same extent as control cells at 1 month after intrastriatal transplantation into newborn rats and generated more neuroblasts. Overexpression of mouse Pax6 in human NSCs also leads to altered levels of lineage-appropriate genes as revealed by Q-PCR. Our data suggest that Pax6 function is conserved between species since its overexpression activates similar genes in mouse and human NSCs. Also, that Pax6 overexpression in striatal NSCs increases the number of neurons but their region-specificity is maintained. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Neuroscience
volume
38
issue
4
pages
616 - 628
publisher
Elsevier
external identifiers
  • wos:000258347300017
  • pmid:18595732
  • scopus:47749138860
ISSN
1044-7431
DOI
10.1016/j.mcn.2008.05.010
language
English
LU publication?
yes
id
0a46789a-af5a-46d7-aacb-e1fca95cafa8 (old id 1181648)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18595732?dopt=Abstract
date added to LUP
2008-08-06 11:01:03
date last changed
2017-03-05 04:18:56
@article{0a46789a-af5a-46d7-aacb-e1fca95cafa8,
  abstract     = {During brain embryogenesis, transcription factors drive stem cells towards neuronal fate. Here we show that the transcription factor Pax6 increased in vitro generation of neurons from striatal but not cortical neural stem cells (NSCs), derived from 6 to 9 weeks old human fetuses, without affecting survival and proliferation. Overexpression of mouse Pax6 produced increased numbers of GABA+ and DARPP-32+ (characteristic of striatum) but not glutamate+ neurons (characteristic of cortex). Pax6-overexpressing cells survived and migrated to the same extent as control cells at 1 month after intrastriatal transplantation into newborn rats and generated more neuroblasts. Overexpression of mouse Pax6 in human NSCs also leads to altered levels of lineage-appropriate genes as revealed by Q-PCR. Our data suggest that Pax6 function is conserved between species since its overexpression activates similar genes in mouse and human NSCs. Also, that Pax6 overexpression in striatal NSCs increases the number of neurons but their region-specificity is maintained.},
  author       = {Kallur, Therese and Gisler, Ramiro and Lindvall, Olle and Kokaia, Zaal},
  issn         = {1044-7431},
  language     = {eng},
  number       = {4},
  pages        = {616--628},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Neuroscience},
  title        = {Pax6 promotes neurogenesis in human neural stem cells.},
  url          = {http://dx.doi.org/10.1016/j.mcn.2008.05.010},
  volume       = {38},
  year         = {2008},
}