Intraventricular Infusion of TrkB-Fc Fusion Protein Promotes Ischemia-Induced Neurogenesis in Adult Rat Dentate Gyrus.
Gustafsson, Elin ; Lindvall, Olle LU and Kokaia, Zaal LU
(2003)
In Stroke: a journal of cerebral circulation
34(11).
p.2710-2715
- Abstract
- Background and Purpose-We have previously shown that delivery of brain-derived neurotrophic factor (BDNF) through direct intrahippocampal gene transduction with a viral vector suppresses the formation of new dentate granule cells triggered by global forebrain ischemia. Here, we investigated whether inhibition of endogenous BDNF alters ischemia-induced neurogenesis in the dentate gyrus. Methods-Rats were subjected to 30 minutes of global forebrain ischemia and then received intraventricular infusion of either the BDNF scavenger, TrkB-Fc fusion protein, or control Hu-Fc for 2 weeks. In parallel, all animals were injected intraperitoneally with the mitosis marker 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU). Animals were killed at 2 or 6... (More)
- Background and Purpose-We have previously shown that delivery of brain-derived neurotrophic factor (BDNF) through direct intrahippocampal gene transduction with a viral vector suppresses the formation of new dentate granule cells triggered by global forebrain ischemia. Here, we investigated whether inhibition of endogenous BDNF alters ischemia-induced neurogenesis in the dentate gyrus. Methods-Rats were subjected to 30 minutes of global forebrain ischemia and then received intraventricular infusion of either the BDNF scavenger, TrkB-Fc fusion protein, or control Hu-Fc for 2 weeks. In parallel, all animals were injected intraperitoneally with the mitosis marker 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU). Animals were killed at 2 or 6 weeks after the ischemic insult, and neurogenesis was then assessed immunocytochemically with epifluorescence or confocal microscopy. Results-Infusion of TrkB-Fc fusion protein gave rise to elevated numbers of ischemia-generated new neurons, double-labeled with BrdU and the early neuronal marker Hu or the mature neuronal marker NeuN, in the dentate subgranular zone and granule cell layer at 2 and 6 weeks after the insult. Conclusions-Our findings provide evidence that endogenous BDNF counteracts neuronal differentiation, but not cell proliferation or survival, in ischemia-induced dentate gyrus neurogenesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/118246
- author
- Gustafsson, Elin
; Lindvall, Olle
LU
and Kokaia, Zaal
LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- global, hippocampus, cerebral ischemia, rats, stroke, neurons, brain-derived neurotrophic factor
- in
- Stroke: a journal of cerebral circulation
- volume
- 34
- issue
- 11
- pages
- 2710 - 2715
- publisher
- American Heart Association
- external identifiers
-
- pmid:14563966
- wos:000186398800037
- scopus:0242694048
- pmid:14563966
- ISSN
- 1524-4628
- DOI
- 10.1161/01.STR.0000096025.35225.36
- language
- English
- LU publication?
- yes
- id
- 449643e1-40e9-403c-ae08-33d982412fdf (old id 118246)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14563966&dopt=Abstract
- date added to LUP
- 2016-04-01 16:35:46
- date last changed
- 2022-03-07 06:59:13
@article{449643e1-40e9-403c-ae08-33d982412fdf,
abstract = {{Background and Purpose-We have previously shown that delivery of brain-derived neurotrophic factor (BDNF) through direct intrahippocampal gene transduction with a viral vector suppresses the formation of new dentate granule cells triggered by global forebrain ischemia. Here, we investigated whether inhibition of endogenous BDNF alters ischemia-induced neurogenesis in the dentate gyrus. Methods-Rats were subjected to 30 minutes of global forebrain ischemia and then received intraventricular infusion of either the BDNF scavenger, TrkB-Fc fusion protein, or control Hu-Fc for 2 weeks. In parallel, all animals were injected intraperitoneally with the mitosis marker 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU). Animals were killed at 2 or 6 weeks after the ischemic insult, and neurogenesis was then assessed immunocytochemically with epifluorescence or confocal microscopy. Results-Infusion of TrkB-Fc fusion protein gave rise to elevated numbers of ischemia-generated new neurons, double-labeled with BrdU and the early neuronal marker Hu or the mature neuronal marker NeuN, in the dentate subgranular zone and granule cell layer at 2 and 6 weeks after the insult. Conclusions-Our findings provide evidence that endogenous BDNF counteracts neuronal differentiation, but not cell proliferation or survival, in ischemia-induced dentate gyrus neurogenesis.}},
author = {{Gustafsson, Elin and Lindvall, Olle and Kokaia, Zaal}},
issn = {{1524-4628}},
keywords = {{global; hippocampus; cerebral ischemia; rats; stroke; neurons; brain-derived neurotrophic factor}},
language = {{eng}},
number = {{11}},
pages = {{2710--2715}},
publisher = {{American Heart Association}},
series = {{Stroke: a journal of cerebral circulation}},
title = {{Intraventricular Infusion of TrkB-Fc Fusion Protein Promotes Ischemia-Induced Neurogenesis in Adult Rat Dentate Gyrus.}},
url = {{http://dx.doi.org/10.1161/01.STR.0000096025.35225.36}},
doi = {{10.1161/01.STR.0000096025.35225.36}},
volume = {{34}},
year = {{2003}},
}