Complementary Signaling through flt3 and Interleukin-7 Receptor {alpha} Is Indispensable for Fetal and Adult B Cell Genesis.
(2003) In Journal of Experimental Medicine 198(10). p.1495-1506- Abstract
- Extensive studies of mice deficient in one or several cytokine receptors have failed to support an indispensable role of cytokines in development of multiple blood cell lineages. Whereas B1 B cells and Igs are sustained at normal levels throughout life of mice deficient in IL-7, IL-7R{alpha}, common cytokine receptor gamma chain, or flt3 ligand (FL), we report here that adult mice double deficient in IL-7R{alpha} and FL completely lack visible LNs, conventional IgM+ B cells, IgA+ plasma cells, and B1 cells, and consequently produce no Igs. All stages of committed B cell progenitors are undetectable in FL-/- x IL-7R{alpha}-/- BM that also lacks expression of the B cell commitment factor Pax5 and its direct target genes. Furthermore, in... (More)
- Extensive studies of mice deficient in one or several cytokine receptors have failed to support an indispensable role of cytokines in development of multiple blood cell lineages. Whereas B1 B cells and Igs are sustained at normal levels throughout life of mice deficient in IL-7, IL-7R{alpha}, common cytokine receptor gamma chain, or flt3 ligand (FL), we report here that adult mice double deficient in IL-7R{alpha} and FL completely lack visible LNs, conventional IgM+ B cells, IgA+ plasma cells, and B1 cells, and consequently produce no Igs. All stages of committed B cell progenitors are undetectable in FL-/- x IL-7R{alpha}-/- BM that also lacks expression of the B cell commitment factor Pax5 and its direct target genes. Furthermore, in contrast to IL-7R{alpha}-/- mice, FL-/- x IL-7R{alpha}-/- mice also lack mature B cells and detectable committed B cell progenitors during fetal development. Thus, signaling through the cytokine tyrosine kinase receptor flt3 and IL-7R{alpha} are indispensable for fetal and adult B cell development. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/119047
- author
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- IL-7 receptor, Flt3 ligand, B1 cells, Pax5, lymphopoiesis
- in
- Journal of Experimental Medicine
- volume
- 198
- issue
- 10
- pages
- 1495 - 1506
- publisher
- Rockefeller University Press
- external identifiers
-
- wos:000186845600005
- pmid:14610045
- scopus:10744221710
- ISSN
- 1540-9538
- DOI
- 10.1084/jem.20031152
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Endocrinology (013241500), Hematopoietic Stem Cell Laboratory (013022012), Immunology (013212020), Neurology, Lund (013027000)
- id
- 193b69e0-16b1-430d-b052-504abf5e6466 (old id 119047)
- date added to LUP
- 2016-04-01 17:11:57
- date last changed
- 2022-07-24 17:05:29
@article{193b69e0-16b1-430d-b052-504abf5e6466, abstract = {{Extensive studies of mice deficient in one or several cytokine receptors have failed to support an indispensable role of cytokines in development of multiple blood cell lineages. Whereas B1 B cells and Igs are sustained at normal levels throughout life of mice deficient in IL-7, IL-7R{alpha}, common cytokine receptor gamma chain, or flt3 ligand (FL), we report here that adult mice double deficient in IL-7R{alpha} and FL completely lack visible LNs, conventional IgM+ B cells, IgA+ plasma cells, and B1 cells, and consequently produce no Igs. All stages of committed B cell progenitors are undetectable in FL-/- x IL-7R{alpha}-/- BM that also lacks expression of the B cell commitment factor Pax5 and its direct target genes. Furthermore, in contrast to IL-7R{alpha}-/- mice, FL-/- x IL-7R{alpha}-/- mice also lack mature B cells and detectable committed B cell progenitors during fetal development. Thus, signaling through the cytokine tyrosine kinase receptor flt3 and IL-7R{alpha} are indispensable for fetal and adult B cell development.}}, author = {{Sitnicka Quinn, Ewa and Brakebusch, Cord and Martensson, Inga-Lill and Svensson Frej, Marcus and Agace, William and Sigvardsson, Mikael and Buza-Vidas, Natalija and Bryder, David and M Cilio, Corrado and Ahlenius, Henrik and Maraskovsky, Eugene and Peschon, Jacques J. and Jacobsen, Sten Eirik W.}}, issn = {{1540-9538}}, keywords = {{IL-7 receptor; Flt3 ligand; B1 cells; Pax5; lymphopoiesis}}, language = {{eng}}, number = {{10}}, pages = {{1495--1506}}, publisher = {{Rockefeller University Press}}, series = {{Journal of Experimental Medicine}}, title = {{Complementary Signaling through flt3 and Interleukin-7 Receptor {alpha} Is Indispensable for Fetal and Adult B Cell Genesis.}}, url = {{https://lup.lub.lu.se/search/files/4904593/623906.pdf}}, doi = {{10.1084/jem.20031152}}, volume = {{198}}, year = {{2003}}, }