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Electrophysiological and morphological characteristics of pheromone receptors in male pine sawflies, Diprion pini (Hymenoptera : Diprionidae), and behavioural response to some compounds

Anderbrant, O. LU ; Hallberg, E. LU ; Geri, C. ; Varama, M. ; Hedenström, E. ; Högberg, H. E. ; Fägerhag, J. ; Edlund, H. LU ; Wassgren, A. B. and Bergström, G. , et al. (1995) In Journal of Insect Physiology 41(5). p.395-401
Abstract

The morphology and physiology of pheromone receptors on the antennae of male pine sawflies, Diprion pini L., were investigated. Using scanning electron microscopy, five sensillar types were recognized. The type shown to be pheromone sensitive has a long (50-70 μm) cuticular hair, is single-walled, and is innervated by 8 or 9 sensory cells as revealed by transmission electron microscopy. Electroantennography (EAG) showed similar activity of the acetate and propionate of (2S,3R,7R)-3,7-dimethyl-2-tridecanol, precursor of the main constituent of the female-produced sex pheromone. No other isomer induced any significant response. Single-sensillum recordings confirmed the results of the EAG, and also showed that several neurons were excited... (More)

The morphology and physiology of pheromone receptors on the antennae of male pine sawflies, Diprion pini L., were investigated. Using scanning electron microscopy, five sensillar types were recognized. The type shown to be pheromone sensitive has a long (50-70 μm) cuticular hair, is single-walled, and is innervated by 8 or 9 sensory cells as revealed by transmission electron microscopy. Electroantennography (EAG) showed similar activity of the acetate and propionate of (2S,3R,7R)-3,7-dimethyl-2-tridecanol, precursor of the main constituent of the female-produced sex pheromone. No other isomer induced any significant response. Single-sensillum recordings confirmed the results of the EAG, and also showed that several neurons were excited by the active compound. EAG recordings and combined gas chromatographic-electroantennographic detection indicated that esters of three 3,7-dimethyl-2-pentadecanol (diprionol) isomers were active, but field tests could not demonstrate any behavioural effect. Diprionol esters are used as sex pheromones by all other pine sawflies investigated so far, and D. pini is thus the first diprionid species shown to use a different sex pheromone.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Behavioural activity, Chiral compound, Electron microscopy, Electrophysiology, Fine structure, Sex pheromone
in
Journal of Insect Physiology
volume
41
issue
5
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:0029103649
ISSN
0022-1910
DOI
10.1016/0022-1910(94)00126-2
project
Chemical communication in sawflies
language
English
LU publication?
yes
id
11909c57-36f9-4dda-a8c9-2d4107c67653
date added to LUP
2020-05-20 15:13:03
date last changed
2024-01-02 11:12:15
@article{11909c57-36f9-4dda-a8c9-2d4107c67653,
  abstract     = {{<p>The morphology and physiology of pheromone receptors on the antennae of male pine sawflies, Diprion pini L., were investigated. Using scanning electron microscopy, five sensillar types were recognized. The type shown to be pheromone sensitive has a long (50-70 μm) cuticular hair, is single-walled, and is innervated by 8 or 9 sensory cells as revealed by transmission electron microscopy. Electroantennography (EAG) showed similar activity of the acetate and propionate of (2S,3R,7R)-3,7-dimethyl-2-tridecanol, precursor of the main constituent of the female-produced sex pheromone. No other isomer induced any significant response. Single-sensillum recordings confirmed the results of the EAG, and also showed that several neurons were excited by the active compound. EAG recordings and combined gas chromatographic-electroantennographic detection indicated that esters of three 3,7-dimethyl-2-pentadecanol (diprionol) isomers were active, but field tests could not demonstrate any behavioural effect. Diprionol esters are used as sex pheromones by all other pine sawflies investigated so far, and D. pini is thus the first diprionid species shown to use a different sex pheromone.</p>}},
  author       = {{Anderbrant, O. and Hallberg, E. and Geri, C. and Varama, M. and Hedenström, E. and Högberg, H. E. and Fägerhag, J. and Edlund, H. and Wassgren, A. B. and Bergström, G. and Hansson, Bill S.}},
  issn         = {{0022-1910}},
  keywords     = {{Behavioural activity; Chiral compound; Electron microscopy; Electrophysiology; Fine structure; Sex pheromone}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{5}},
  pages        = {{395--401}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Insect Physiology}},
  title        = {{Electrophysiological and morphological characteristics of pheromone receptors in male pine sawflies, Diprion pini (Hymenoptera : Diprionidae), and behavioural response to some compounds}},
  url          = {{http://dx.doi.org/10.1016/0022-1910(94)00126-2}},
  doi          = {{10.1016/0022-1910(94)00126-2}},
  volume       = {{41}},
  year         = {{1995}},
}