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Cyclodextrin-surfactant complex: A new route in DNA decompaction

Gonzalez Perez, Alfredo LU ; Dias, Rita LU ; Nylander, Tommy LU and Lindman, Björn LU (2008) In Biomacromolecules 9(3). p.772-775
Abstract
In the present work, we show a new approach for decompaction of DNA-cationic surfactant complexes, e.g., lipoplexes, by using beta-cyclodextrin (beta-CD). The DNA decompaction was achieved by dissolving the surfactant aggregates in the complex by making use of the high affinity between the beta-CD and the free surfactant in solution. The results from fluorescence microscopy and adiabatic compressibility measurements indicate that coils and globules do not coexist. The reported procedure using beta-CD is an efficient way to decompact DNA surfactant complexes because the association constant of surfactants with beta-CD is large. The surfactant's interaction with beta-CD is specific and the nonspecific interaction between beta-CD and... (More)
In the present work, we show a new approach for decompaction of DNA-cationic surfactant complexes, e.g., lipoplexes, by using beta-cyclodextrin (beta-CD). The DNA decompaction was achieved by dissolving the surfactant aggregates in the complex by making use of the high affinity between the beta-CD and the free surfactant in solution. The results from fluorescence microscopy and adiabatic compressibility measurements indicate that coils and globules do not coexist. The reported procedure using beta-CD is an efficient way to decompact DNA surfactant complexes because the association constant of surfactants with beta-CD is large. The surfactant's interaction with beta-CD is specific and the nonspecific interaction between beta-CD and biological interfaces is small. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
9
issue
3
pages
772 - 775
publisher
The American Chemical Society
external identifiers
  • wos:000253926400003
  • scopus:41949138209
ISSN
1526-4602
DOI
10.1021/bm7012907
language
English
LU publication?
yes
id
3ac813a2-161d-435e-8561-76583edf4d4e (old id 1191576)
date added to LUP
2008-09-05 10:51:01
date last changed
2017-01-15 03:43:00
@article{3ac813a2-161d-435e-8561-76583edf4d4e,
  abstract     = {In the present work, we show a new approach for decompaction of DNA-cationic surfactant complexes, e.g., lipoplexes, by using beta-cyclodextrin (beta-CD). The DNA decompaction was achieved by dissolving the surfactant aggregates in the complex by making use of the high affinity between the beta-CD and the free surfactant in solution. The results from fluorescence microscopy and adiabatic compressibility measurements indicate that coils and globules do not coexist. The reported procedure using beta-CD is an efficient way to decompact DNA surfactant complexes because the association constant of surfactants with beta-CD is large. The surfactant's interaction with beta-CD is specific and the nonspecific interaction between beta-CD and biological interfaces is small.},
  author       = {Gonzalez Perez, Alfredo and Dias, Rita and Nylander, Tommy and Lindman, Björn},
  issn         = {1526-4602},
  language     = {eng},
  number       = {3},
  pages        = {772--775},
  publisher    = {The American Chemical Society},
  series       = {Biomacromolecules},
  title        = {Cyclodextrin-surfactant complex: A new route in DNA decompaction},
  url          = {http://dx.doi.org/10.1021/bm7012907},
  volume       = {9},
  year         = {2008},
}