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Induction of neurites by the regulatory domains of PKCdelta and epsilon is counteracted by PKC catalytic activity and by the RhoA pathway.

Ling, Mia LU ; Trollér, Ulrika LU ; Zeidman, Ruth LU ; Lundberg, Cecilia LU and Larsson, Christer LU (2004) In Experimental Cell Research 292(1). p.135-150
Abstract
We have shown that protein kinase C (PKC) var epsilon, independently of its kinase activity, via its regulatory domain (RD), induces neurites in neuroblastoma cells. This study was designed to evaluate whether the same effect is obtained in nonmalignant neural cells and to dissect mechanisms mediating the effect. Overexpression of PKCvar epsilon resulted in neurite induction in two immortalised neural cell lines (HiB5 and RN33B). Phorbol ester potentiated neurite outgrowth from PKCvar epsilon-overexpressing cells and led to neurite induction in cells overexpressing PKCδ. The effects were potentiated by blocking the PKC catalytic activity with GF109203X. Furthermore, kinase-inactive PKCδ induced more neurites than the wild-type isoform. The... (More)
We have shown that protein kinase C (PKC) var epsilon, independently of its kinase activity, via its regulatory domain (RD), induces neurites in neuroblastoma cells. This study was designed to evaluate whether the same effect is obtained in nonmalignant neural cells and to dissect mechanisms mediating the effect. Overexpression of PKCvar epsilon resulted in neurite induction in two immortalised neural cell lines (HiB5 and RN33B). Phorbol ester potentiated neurite outgrowth from PKCvar epsilon-overexpressing cells and led to neurite induction in cells overexpressing PKCδ. The effects were potentiated by blocking the PKC catalytic activity with GF109203X. Furthermore, kinase-inactive PKCδ induced more neurites than the wild-type isoform. The isolated regulatory domains of novel PKC isoforms also induced neurites. Experiments with PKCδ-overexpressing HiB5 cells demonstrated that phorbol ester, even in the presence of a PKC inhibitor, led to a decrease in stress fibres, indicating an inactivation of RhoA. Active RhoA blocked PKC-induced neurite outgrowth, and inhibition of the RhoA effector ROCK led to neurite outgrowth. This demonstrates that neurite induction by the regulatory domain of PKCδ can be counteracted by PKCδ kinase activity, that PKC-induced neurite outgrowth is accompanied by stress fibre dismantling indicating an inactivation of RhoA, and that the RhoA pathway suppresses PKC-mediated neurite outgrowth. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
RhoA, Neurite outgrowth, Protein kinase C, Stress fibres
in
Experimental Cell Research
volume
292
issue
1
pages
135 - 150
publisher
Academic Press
external identifiers
  • pmid:14720513
  • wos:000187777800013
  • scopus:0346725844
ISSN
1090-2422
DOI
10.1016/j.yexcr.2003.08.013
language
English
LU publication?
yes
id
4aadc8a0-7275-4370-8ba8-728f6570df02 (old id 120165)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14720513&dopt=Abstract
date added to LUP
2007-07-17 09:27:09
date last changed
2017-09-24 03:43:05
@article{4aadc8a0-7275-4370-8ba8-728f6570df02,
  abstract     = {We have shown that protein kinase C (PKC) var epsilon, independently of its kinase activity, via its regulatory domain (RD), induces neurites in neuroblastoma cells. This study was designed to evaluate whether the same effect is obtained in nonmalignant neural cells and to dissect mechanisms mediating the effect. Overexpression of PKCvar epsilon resulted in neurite induction in two immortalised neural cell lines (HiB5 and RN33B). Phorbol ester potentiated neurite outgrowth from PKCvar epsilon-overexpressing cells and led to neurite induction in cells overexpressing PKCδ. The effects were potentiated by blocking the PKC catalytic activity with GF109203X. Furthermore, kinase-inactive PKCδ induced more neurites than the wild-type isoform. The isolated regulatory domains of novel PKC isoforms also induced neurites. Experiments with PKCδ-overexpressing HiB5 cells demonstrated that phorbol ester, even in the presence of a PKC inhibitor, led to a decrease in stress fibres, indicating an inactivation of RhoA. Active RhoA blocked PKC-induced neurite outgrowth, and inhibition of the RhoA effector ROCK led to neurite outgrowth. This demonstrates that neurite induction by the regulatory domain of PKCδ can be counteracted by PKCδ kinase activity, that PKC-induced neurite outgrowth is accompanied by stress fibre dismantling indicating an inactivation of RhoA, and that the RhoA pathway suppresses PKC-mediated neurite outgrowth.},
  author       = {Ling, Mia and Trollér, Ulrika and Zeidman, Ruth and Lundberg, Cecilia and Larsson, Christer},
  issn         = {1090-2422},
  keyword      = {RhoA,Neurite outgrowth,Protein kinase C,Stress fibres},
  language     = {eng},
  number       = {1},
  pages        = {135--150},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {Induction of neurites by the regulatory domains of PKCdelta and epsilon is counteracted by PKC catalytic activity and by the RhoA pathway.},
  url          = {http://dx.doi.org/10.1016/j.yexcr.2003.08.013},
  volume       = {292},
  year         = {2004},
}