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FGF-8 is involved in bone metastasis of prostate cancer

Valta, Maija P ; Tuomela, Johanna ; Bjartell, Anders LU ; Valve, Eeva ; Vaeaenaenen, H Kalervo and Härkönen, Pirkko LU (2008) In International Journal of Cancer 123(1). p.22-31
Abstract
Prostate cancer is the most common malignancy of men in Western countries. Patients with advanced prostate cancer suffer from incurable bone metastases. Recent data indicate that interactions between prostate cancer cells, osteoblasts, osteoclasts and the bone matrix are essential in the formation of bone metastases. FGF-8 is widely overexpressed in prostate cancer. Recently, FGF-8 has been found to affect both osteoblast and osteoclast differentiation. The aim of this study was to examine the role of FGF-8 in bone metastasis of prostate cancer. Immunohistochemistry was used to analyse FGF-8 expression in clinical samples of prostate cancer bone metastases. The functional significance of FGF-8 in growth of bone metastasis and formation of... (More)
Prostate cancer is the most common malignancy of men in Western countries. Patients with advanced prostate cancer suffer from incurable bone metastases. Recent data indicate that interactions between prostate cancer cells, osteoblasts, osteoclasts and the bone matrix are essential in the formation of bone metastases. FGF-8 is widely overexpressed in prostate cancer. Recently, FGF-8 has been found to affect both osteoblast and osteoclast differentiation. The aim of this study was to examine the role of FGF-8 in bone metastasis of prostate cancer. Immunohistochemistry was used to analyse FGF-8 expression in clinical samples of prostate cancer bone metastases. The functional significance of FGF-8 in growth of bone metastasis and formation of bone lesions was verified by using intratibial inoculations of FGF-8 or mock transfected PC-3 prostate cancer cells in nude mice. Intratibial tumors and bone lesions were analysed with X-ray, micro-CT and detailed histomorphometry using image analysis software and with immunostaining for osteocalcin and cathepsin K. Immunohistochemical analysis of tissue microarray of bone metastases of human prostate cancer showed that 76% of human bone metastasis samples (n = 25 from 11 patients) were positive for FGF-8. FGF-8 increased the growth of intratibial tumors and local formation of lvtic and sclerotic lesions of bone. These results demonstrate that PGF-8 is expressed at a high frequency in bone metastases of human prostate cancer and that expression of FGF-8 in PC-3 prostate cancer cells increases their growth as intratibial tumors and modulates formation of bone lesions in an in vivo model of prostate cancer bone metastasis. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bone metastasis, FGF-8, prostate cancer, PC-3 cells
in
International Journal of Cancer
volume
123
issue
1
pages
22 - 31
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000255973200005
  • scopus:43949123830
  • pmid:18386787
ISSN
0020-7136
DOI
10.1002/ijc.23422
language
English
LU publication?
yes
additional info
Department affilation moved from v1000588 (Tumour Biology, Malmö) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:39:27.
id
a155934d-952c-4e03-bd86-f3864329666b (old id 1203380)
date added to LUP
2016-04-01 12:16:40
date last changed
2022-01-27 01:23:03
@article{a155934d-952c-4e03-bd86-f3864329666b,
  abstract     = {{Prostate cancer is the most common malignancy of men in Western countries. Patients with advanced prostate cancer suffer from incurable bone metastases. Recent data indicate that interactions between prostate cancer cells, osteoblasts, osteoclasts and the bone matrix are essential in the formation of bone metastases. FGF-8 is widely overexpressed in prostate cancer. Recently, FGF-8 has been found to affect both osteoblast and osteoclast differentiation. The aim of this study was to examine the role of FGF-8 in bone metastasis of prostate cancer. Immunohistochemistry was used to analyse FGF-8 expression in clinical samples of prostate cancer bone metastases. The functional significance of FGF-8 in growth of bone metastasis and formation of bone lesions was verified by using intratibial inoculations of FGF-8 or mock transfected PC-3 prostate cancer cells in nude mice. Intratibial tumors and bone lesions were analysed with X-ray, micro-CT and detailed histomorphometry using image analysis software and with immunostaining for osteocalcin and cathepsin K. Immunohistochemical analysis of tissue microarray of bone metastases of human prostate cancer showed that 76% of human bone metastasis samples (n = 25 from 11 patients) were positive for FGF-8. FGF-8 increased the growth of intratibial tumors and local formation of lvtic and sclerotic lesions of bone. These results demonstrate that PGF-8 is expressed at a high frequency in bone metastases of human prostate cancer and that expression of FGF-8 in PC-3 prostate cancer cells increases their growth as intratibial tumors and modulates formation of bone lesions in an in vivo model of prostate cancer bone metastasis.}},
  author       = {{Valta, Maija P and Tuomela, Johanna and Bjartell, Anders and Valve, Eeva and Vaeaenaenen, H Kalervo and Härkönen, Pirkko}},
  issn         = {{0020-7136}},
  keywords     = {{bone metastasis; FGF-8; prostate cancer; PC-3 cells}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{22--31}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{International Journal of Cancer}},
  title        = {{FGF-8 is involved in bone metastasis of prostate cancer}},
  url          = {{http://dx.doi.org/10.1002/ijc.23422}},
  doi          = {{10.1002/ijc.23422}},
  volume       = {{123}},
  year         = {{2008}},
}