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Pulsed electric field reduces the permeability of potato cell wall

Gomez, Federico LU ; Vernier, P Thomas; Dejmek, Petr LU ; Vicente, Antonio and Gundersen, Martin A (2008) In Bioelectromagnetics 29(4). p.296-301
Abstract
The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FMI-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
oxidative cross-linking, stress, electric fields, cell wall, FM1-43
in
Bioelectromagnetics
volume
29
issue
4
pages
296 - 301
publisher
John Wiley & Sons
external identifiers
  • wos:000255498900006
  • scopus:42949122958
ISSN
0197-8462
DOI
10.1002/bem.20394
language
English
LU publication?
yes
id
d67a0f34-ed47-4dc4-a452-8a48eeef13a9 (old id 1205373)
date added to LUP
2008-09-18 14:56:08
date last changed
2017-07-09 03:44:21
@article{d67a0f34-ed47-4dc4-a452-8a48eeef13a9,
  abstract     = {The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FMI-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.},
  author       = {Gomez, Federico and Vernier, P Thomas and Dejmek, Petr and Vicente, Antonio and Gundersen, Martin A},
  issn         = {0197-8462},
  keyword      = {oxidative cross-linking,stress,electric fields,cell wall,FM1-43},
  language     = {eng},
  number       = {4},
  pages        = {296--301},
  publisher    = {John Wiley & Sons},
  series       = {Bioelectromagnetics},
  title        = {Pulsed electric field reduces the permeability of potato cell wall},
  url          = {http://dx.doi.org/10.1002/bem.20394},
  volume       = {29},
  year         = {2008},
}