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Pulsed electric field reduces the permeability of potato cell wall

Gomez, Federico LU ; Vernier, P Thomas ; Dejmek, Petr LU orcid ; Vicente, Antonio and Gundersen, Martin A (2008) In Bioelectromagnetics 29(4). p.296-301
Abstract
The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FMI-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
oxidative cross-linking, stress, electric fields, cell wall, FM1-43
in
Bioelectromagnetics
volume
29
issue
4
pages
296 - 301
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000255498900006
  • scopus:42949122958
  • pmid:18163439
ISSN
0197-8462
DOI
10.1002/bem.20394
language
English
LU publication?
yes
id
d67a0f34-ed47-4dc4-a452-8a48eeef13a9 (old id 1205373)
date added to LUP
2016-04-01 12:22:46
date last changed
2023-11-11 22:51:07
@article{d67a0f34-ed47-4dc4-a452-8a48eeef13a9,
  abstract     = {{The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FMI-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.}},
  author       = {{Gomez, Federico and Vernier, P Thomas and Dejmek, Petr and Vicente, Antonio and Gundersen, Martin A}},
  issn         = {{0197-8462}},
  keywords     = {{oxidative cross-linking; stress; electric fields; cell wall; FM1-43}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{296--301}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Bioelectromagnetics}},
  title        = {{Pulsed electric field reduces the permeability of potato cell wall}},
  url          = {{http://dx.doi.org/10.1002/bem.20394}},
  doi          = {{10.1002/bem.20394}},
  volume       = {{29}},
  year         = {{2008}},
}