Ultrastructural and antigenic properties of neural stem cells and their progeny in adult rat subventricular zone.
Danilov, Alexandre LU ; Gomes-Leal, Wallace LU ; Ahlenius, Henrik LU ; Kokaia, Zaal LU
; Carlemalm, Eric
LU
and Lindvall, Olle
LU
(2009)
In GLIA
Aug 15.
p.136-152
- Abstract
- Neural stem cells (NSCs) in the subventricular zone (SVZ) continuously generate olfactory bulb interneurons in the adult rodent brain. Based on their ultrastructural and antigenic properties, NSCs, transient amplifying precursor cells, and neuroblasts (B, C, and A cells, respectively) have been distinguished in mouse SVZ. Here, we aimed to identify these cell types in rat SVZ ultrastructurally and at the light microscopy level, and to determine the antigenic properties of each cell type using gold and fluorescence immunolabeling. We found astrocytes with single cilia (NSCs, correspond to B cells) and neuroblasts (A cells). We also observed mitotic cells, ependymal cells, displaced ependymal cells, and mature astrocytes. In contrast,... (More)
- Neural stem cells (NSCs) in the subventricular zone (SVZ) continuously generate olfactory bulb interneurons in the adult rodent brain. Based on their ultrastructural and antigenic properties, NSCs, transient amplifying precursor cells, and neuroblasts (B, C, and A cells, respectively) have been distinguished in mouse SVZ. Here, we aimed to identify these cell types in rat SVZ ultrastructurally and at the light microscopy level, and to determine the antigenic properties of each cell type using gold and fluorescence immunolabeling. We found astrocytes with single cilia (NSCs, correspond to B cells) and neuroblasts (A cells). We also observed mitotic cells, ependymal cells, displaced ependymal cells, and mature astrocytes. In contrast, transient amplifying precursor cells (C cells) were not detected. The NSCs and neuroblasts had epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor alpha (PDGFRalpha) expressed on the ciliary apparatus and were the only cell types incorporating the proliferation marker BrdU. Throughout mitosis, EGFR and PDGFRalpha were associated with the microtubule of the mitotic spindle. Ependymal and displaced ependymal cells also expressed EGFR and PDGFRalpha on their cilia but did not incorporate BrdU. Our findings indicate that the NSCs in adult rat SVZ give rise directly to neuroblasts. During mitosis, the NSCs disassemble the primary cilium and symmetrically distribute EGFR and PDGFRalpha among their progeny. (c) 2008 Wiley-Liss, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1223114
- author
- Danilov, Alexandre
LU
; Gomes-Leal, Wallace
LU
; Ahlenius, Henrik
LU
; Kokaia, Zaal
LU
; Carlemalm, Eric
LU
and Lindvall, Olle
LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- GLIA
- volume
- Aug 15
- pages
- 136 - 152
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000261681700002
- pmid:18709646
- scopus:60549097297
- ISSN
- 1098-1136
- DOI
- 10.1002/glia.20741
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neurology, Lund (013027000), Electron Microscopy Unit (013100002)
- id
- aeb57c44-d53b-4349-9d3e-fa309adccca6 (old id 1223114)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18709646?dopt=Abstract
- date added to LUP
- 2016-04-04 07:03:26
- date last changed
- 2022-01-29 01:38:45
@article{aeb57c44-d53b-4349-9d3e-fa309adccca6,
abstract = {{Neural stem cells (NSCs) in the subventricular zone (SVZ) continuously generate olfactory bulb interneurons in the adult rodent brain. Based on their ultrastructural and antigenic properties, NSCs, transient amplifying precursor cells, and neuroblasts (B, C, and A cells, respectively) have been distinguished in mouse SVZ. Here, we aimed to identify these cell types in rat SVZ ultrastructurally and at the light microscopy level, and to determine the antigenic properties of each cell type using gold and fluorescence immunolabeling. We found astrocytes with single cilia (NSCs, correspond to B cells) and neuroblasts (A cells). We also observed mitotic cells, ependymal cells, displaced ependymal cells, and mature astrocytes. In contrast, transient amplifying precursor cells (C cells) were not detected. The NSCs and neuroblasts had epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor alpha (PDGFRalpha) expressed on the ciliary apparatus and were the only cell types incorporating the proliferation marker BrdU. Throughout mitosis, EGFR and PDGFRalpha were associated with the microtubule of the mitotic spindle. Ependymal and displaced ependymal cells also expressed EGFR and PDGFRalpha on their cilia but did not incorporate BrdU. Our findings indicate that the NSCs in adult rat SVZ give rise directly to neuroblasts. During mitosis, the NSCs disassemble the primary cilium and symmetrically distribute EGFR and PDGFRalpha among their progeny. (c) 2008 Wiley-Liss, Inc.}},
author = {{Danilov, Alexandre and Gomes-Leal, Wallace and Ahlenius, Henrik and Kokaia, Zaal and Carlemalm, Eric and Lindvall, Olle}},
issn = {{1098-1136}},
language = {{eng}},
pages = {{136--152}},
publisher = {{John Wiley & Sons Inc.}},
series = {{GLIA}},
title = {{Ultrastructural and antigenic properties of neural stem cells and their progeny in adult rat subventricular zone.}},
url = {{http://dx.doi.org/10.1002/glia.20741}},
doi = {{10.1002/glia.20741}},
volume = {{Aug 15}},
year = {{2009}},
}