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Ultrastructural and antigenic properties of neural stem cells and their progeny in adult rat subventricular zone.

Danilov, Alexandre LU ; Gomes-Leal, Wallace LU ; Ahlenius, Henrik LU ; Kokaia, Zaal LU ; Carlemalm, Eric LU and Lindvall, Olle LU (2009) In GLIA Aug 15. p.136-152
Abstract
Neural stem cells (NSCs) in the subventricular zone (SVZ) continuously generate olfactory bulb interneurons in the adult rodent brain. Based on their ultrastructural and antigenic properties, NSCs, transient amplifying precursor cells, and neuroblasts (B, C, and A cells, respectively) have been distinguished in mouse SVZ. Here, we aimed to identify these cell types in rat SVZ ultrastructurally and at the light microscopy level, and to determine the antigenic properties of each cell type using gold and fluorescence immunolabeling. We found astrocytes with single cilia (NSCs, correspond to B cells) and neuroblasts (A cells). We also observed mitotic cells, ependymal cells, displaced ependymal cells, and mature astrocytes. In contrast,... (More)
Neural stem cells (NSCs) in the subventricular zone (SVZ) continuously generate olfactory bulb interneurons in the adult rodent brain. Based on their ultrastructural and antigenic properties, NSCs, transient amplifying precursor cells, and neuroblasts (B, C, and A cells, respectively) have been distinguished in mouse SVZ. Here, we aimed to identify these cell types in rat SVZ ultrastructurally and at the light microscopy level, and to determine the antigenic properties of each cell type using gold and fluorescence immunolabeling. We found astrocytes with single cilia (NSCs, correspond to B cells) and neuroblasts (A cells). We also observed mitotic cells, ependymal cells, displaced ependymal cells, and mature astrocytes. In contrast, transient amplifying precursor cells (C cells) were not detected. The NSCs and neuroblasts had epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor alpha (PDGFRalpha) expressed on the ciliary apparatus and were the only cell types incorporating the proliferation marker BrdU. Throughout mitosis, EGFR and PDGFRalpha were associated with the microtubule of the mitotic spindle. Ependymal and displaced ependymal cells also expressed EGFR and PDGFRalpha on their cilia but did not incorporate BrdU. Our findings indicate that the NSCs in adult rat SVZ give rise directly to neuroblasts. During mitosis, the NSCs disassemble the primary cilium and symmetrically distribute EGFR and PDGFRalpha among their progeny. (c) 2008 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
GLIA
volume
Aug 15
pages
136 - 152
publisher
John Wiley & Sons
external identifiers
  • wos:000261681700002
  • pmid:18709646
  • scopus:60549097297
ISSN
1098-1136
DOI
10.1002/glia.20741
language
English
LU publication?
yes
id
aeb57c44-d53b-4349-9d3e-fa309adccca6 (old id 1223114)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18709646?dopt=Abstract
date added to LUP
2008-09-02 14:21:52
date last changed
2017-11-05 04:40:08
@article{aeb57c44-d53b-4349-9d3e-fa309adccca6,
  abstract     = {Neural stem cells (NSCs) in the subventricular zone (SVZ) continuously generate olfactory bulb interneurons in the adult rodent brain. Based on their ultrastructural and antigenic properties, NSCs, transient amplifying precursor cells, and neuroblasts (B, C, and A cells, respectively) have been distinguished in mouse SVZ. Here, we aimed to identify these cell types in rat SVZ ultrastructurally and at the light microscopy level, and to determine the antigenic properties of each cell type using gold and fluorescence immunolabeling. We found astrocytes with single cilia (NSCs, correspond to B cells) and neuroblasts (A cells). We also observed mitotic cells, ependymal cells, displaced ependymal cells, and mature astrocytes. In contrast, transient amplifying precursor cells (C cells) were not detected. The NSCs and neuroblasts had epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor alpha (PDGFRalpha) expressed on the ciliary apparatus and were the only cell types incorporating the proliferation marker BrdU. Throughout mitosis, EGFR and PDGFRalpha were associated with the microtubule of the mitotic spindle. Ependymal and displaced ependymal cells also expressed EGFR and PDGFRalpha on their cilia but did not incorporate BrdU. Our findings indicate that the NSCs in adult rat SVZ give rise directly to neuroblasts. During mitosis, the NSCs disassemble the primary cilium and symmetrically distribute EGFR and PDGFRalpha among their progeny. (c) 2008 Wiley-Liss, Inc.},
  author       = {Danilov, Alexandre and Gomes-Leal, Wallace and Ahlenius, Henrik and Kokaia, Zaal and Carlemalm, Eric and Lindvall, Olle},
  issn         = {1098-1136},
  language     = {eng},
  pages        = {136--152},
  publisher    = {John Wiley & Sons},
  series       = {GLIA},
  title        = {Ultrastructural and antigenic properties of neural stem cells and their progeny in adult rat subventricular zone.},
  url          = {http://dx.doi.org/10.1002/glia.20741},
  volume       = {Aug 15},
  year         = {2009},
}