Disturbed cholesterol homeostasis in hormone-sensitive lipase null mice.
Fernandez, Celine LU ; Lindholm, Marie LU ; Krogh, Morten LU ; Lucas, Stephanie LU ; Larsson, Sara LU ; Osmark, Peter LU ; Berger, Karin LU
; Boren, Jan
; Fielding, Barbara A
and Frayn, Keith N
, et al.
(2008)
In American Journal of Physiology: Endocrinology and Metabolism
295(July 29).
p.820-831
- Abstract
- Transcriptomics analysis revealed that genes involved in hepatic de novo cholesterol synthesis were down-regulated in fed HSL-null mice that had been on high-fat diet (HFD) for 6 months. This finding prompted a further analysis of cholesterol metabolism in HSL-null mice, which was performed in fed and 16-h fasted mice on a normal chow diet (ND) or a HFD regimen. Plasma cholesterol was elevated in HSL-null mice, in all tested conditions, as a result of cholesterol enrichment of HDL and VLDL. Hepatic esterified cholesterol content and ATP binding cassette transporter A1 (ABCA1) mRNA and protein levels were increased in HSL-null mice regardless of the dietary regimen. Unsaturated fatty acid composition of hepatic triglycerides was modified in... (More)
- Transcriptomics analysis revealed that genes involved in hepatic de novo cholesterol synthesis were down-regulated in fed HSL-null mice that had been on high-fat diet (HFD) for 6 months. This finding prompted a further analysis of cholesterol metabolism in HSL-null mice, which was performed in fed and 16-h fasted mice on a normal chow diet (ND) or a HFD regimen. Plasma cholesterol was elevated in HSL-null mice, in all tested conditions, as a result of cholesterol enrichment of HDL and VLDL. Hepatic esterified cholesterol content and ATP binding cassette transporter A1 (ABCA1) mRNA and protein levels were increased in HSL-null mice regardless of the dietary regimen. Unsaturated fatty acid composition of hepatic triglycerides was modified in fasted HSL-null mice on ND and HFD. The increased ABCA1 expression had no major effect on cholesterol efflux from HSL-null mouse hepatocytes. Taken together, the results of this study suggest that HSL plays a critical role in the hydrolysis of cytosolic cholesteryl esters and that increased levels of hepatic cholesteryl esters, due to lack of action of HSL in the liver, is the main mechanism underlying the imbalance in cholesterol metabolism in HSL-null mice. Key words: lipoproteins, unsaturated fatty acids, high-fat diet, microarray. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1223701
- author
- Fernandez, Celine
LU
; Lindholm, Marie
LU
; Krogh, Morten
LU
; Lucas, Stephanie
LU
; Larsson, Sara
LU
; Osmark, Peter
LU
; Berger, Karin
LU
; Boren, Jan
; Fielding, Barbara A
and Frayn, Keith N
, et al. (More)
- Fernandez, Celine
LU
; Lindholm, Marie
LU
; Krogh, Morten
LU
; Lucas, Stephanie
LU
; Larsson, Sara
LU
; Osmark, Peter
LU
; Berger, Karin
LU
; Boren, Jan
; Fielding, Barbara A
; Frayn, Keith N
and Holm, Cecilia
LU
(Less)
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- American Journal of Physiology: Endocrinology and Metabolism
- volume
- 295
- issue
- July 29
- pages
- 820 - 831
- publisher
- American Physiological Society
- external identifiers
-
- pmid:18664600
- scopus:56049108571
- pmid:18664600
- ISSN
- 1522-1555
- DOI
- 10.1152/ajpendo.90206.2008
- language
- English
- LU publication?
- yes
- id
- 5cf8018f-975b-4698-aa2c-d6fc033e9206 (old id 1223701)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18664600?dopt=Abstract
- date added to LUP
- 2016-04-04 09:45:32
- date last changed
- 2024-01-12 17:54:27
@article{5cf8018f-975b-4698-aa2c-d6fc033e9206,
abstract = {{Transcriptomics analysis revealed that genes involved in hepatic de novo cholesterol synthesis were down-regulated in fed HSL-null mice that had been on high-fat diet (HFD) for 6 months. This finding prompted a further analysis of cholesterol metabolism in HSL-null mice, which was performed in fed and 16-h fasted mice on a normal chow diet (ND) or a HFD regimen. Plasma cholesterol was elevated in HSL-null mice, in all tested conditions, as a result of cholesterol enrichment of HDL and VLDL. Hepatic esterified cholesterol content and ATP binding cassette transporter A1 (ABCA1) mRNA and protein levels were increased in HSL-null mice regardless of the dietary regimen. Unsaturated fatty acid composition of hepatic triglycerides was modified in fasted HSL-null mice on ND and HFD. The increased ABCA1 expression had no major effect on cholesterol efflux from HSL-null mouse hepatocytes. Taken together, the results of this study suggest that HSL plays a critical role in the hydrolysis of cytosolic cholesteryl esters and that increased levels of hepatic cholesteryl esters, due to lack of action of HSL in the liver, is the main mechanism underlying the imbalance in cholesterol metabolism in HSL-null mice. Key words: lipoproteins, unsaturated fatty acids, high-fat diet, microarray.}},
author = {{Fernandez, Celine and Lindholm, Marie and Krogh, Morten and Lucas, Stephanie and Larsson, Sara and Osmark, Peter and Berger, Karin and Boren, Jan and Fielding, Barbara A and Frayn, Keith N and Holm, Cecilia}},
issn = {{1522-1555}},
language = {{eng}},
number = {{July 29}},
pages = {{820--831}},
publisher = {{American Physiological Society}},
series = {{American Journal of Physiology: Endocrinology and Metabolism}},
title = {{Disturbed cholesterol homeostasis in hormone-sensitive lipase null mice.}},
url = {{http://dx.doi.org/10.1152/ajpendo.90206.2008}},
doi = {{10.1152/ajpendo.90206.2008}},
volume = {{295}},
year = {{2008}},
}