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Activity-dependent volume transmission by transgene NPY attenuates glutamate release and LTP in subiculum

Toft Sörensen, Andreas LU ; Kanter Schlifke, Irene LU ; Lin, En-Ju D.; During, Matthew J. and Kokaia, Merab LU (2008) In Molecular and Cellular Neuroscience 39(2). p.37-229
Abstract
Neuropeptide Y (NPY) gene transduction of the brain using viral vectors in epileptogenic regions can effectively suppress seizures in animals, and is being considered as a promising alternative treatment strategy for epilepsy. Therefore, it is fundamental to understand the detailed mechanisms governing the release and action of transgene NPY in neuronal circuitries. Using whole-cell recordings from subicular neurons, we show that in animals transduced by recombinant adeno-associated viral (rAAV) vector carrying the NPY gene, transgene NPY is released during high-frequency activation of CA1-subicular synapses. Released transgene NPY attenuates excitatory synaptic transmission not only in activated, but also in neighboring, non-activated... (More)
Neuropeptide Y (NPY) gene transduction of the brain using viral vectors in epileptogenic regions can effectively suppress seizures in animals, and is being considered as a promising alternative treatment strategy for epilepsy. Therefore, it is fundamental to understand the detailed mechanisms governing the release and action of transgene NPY in neuronal circuitries. Using whole-cell recordings from subicular neurons, we show that in animals transduced by recombinant adeno-associated viral (rAAV) vector carrying the NPY gene, transgene NPY is released during high-frequency activation of CA1-subicular synapses. Released transgene NPY attenuates excitatory synaptic transmission not only in activated, but also in neighboring, non-activated synapses. Such broad action of transgene NPY may prevent recruitment of excitatory synapses in epileptic activity and could play a key role in limiting the spread and generalization of seizures. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Long-term potentiation (LTP), Activity-dependent release, Synaptic transmission, Synaptic plasticity, Neuropeptide Y, Gene therapy, Whole-cell patch-clamp, Subiculum
in
Molecular and Cellular Neuroscience
volume
39
issue
2
pages
37 - 229
publisher
Elsevier
external identifiers
  • scopus:52249108911
  • wos:000259874200010
  • pmid:18652899
ISSN
1044-7431
DOI
10.1016/j.mcn.2008.06.014
language
English
LU publication?
yes
id
7c2e2e92-3376-4cf2-9046-300d8a861554 (old id 1237978)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18652899
http://www.sciencedirect.com/science/article/pii/S1044743108001668
date added to LUP
2013-08-07 12:39:37
date last changed
2017-09-19 14:58:15
@article{7c2e2e92-3376-4cf2-9046-300d8a861554,
  abstract     = {Neuropeptide Y (NPY) gene transduction of the brain using viral vectors in epileptogenic regions can effectively suppress seizures in animals, and is being considered as a promising alternative treatment strategy for epilepsy. Therefore, it is fundamental to understand the detailed mechanisms governing the release and action of transgene NPY in neuronal circuitries. Using whole-cell recordings from subicular neurons, we show that in animals transduced by recombinant adeno-associated viral (rAAV) vector carrying the NPY gene, transgene NPY is released during high-frequency activation of CA1-subicular synapses. Released transgene NPY attenuates excitatory synaptic transmission not only in activated, but also in neighboring, non-activated synapses. Such broad action of transgene NPY may prevent recruitment of excitatory synapses in epileptic activity and could play a key role in limiting the spread and generalization of seizures.},
  author       = {Toft Sörensen, Andreas and Kanter Schlifke, Irene and Lin, En-Ju D. and During, Matthew J. and Kokaia, Merab},
  issn         = {1044-7431},
  keyword      = {Long-term potentiation (LTP),Activity-dependent release,Synaptic transmission,Synaptic plasticity,Neuropeptide Y,Gene therapy,Whole-cell patch-clamp,Subiculum},
  language     = {eng},
  number       = {2},
  pages        = {37--229},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Neuroscience},
  title        = {Activity-dependent volume transmission by transgene NPY attenuates glutamate release and LTP in subiculum},
  url          = {http://dx.doi.org/10.1016/j.mcn.2008.06.014},
  volume       = {39},
  year         = {2008},
}