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Induced Growth and Maturation of the Gastrointestinal Tract After Phaseolus vulgaris Lectin Exposure in Suckling Rats

Linderoth, Ann LU ; Biernat, Marzena; Prykhodko, Olena LU ; Kornilovska, Iryna; Pusztai, Arpad; Pierzynowski, Stefan LU and Weström, Björn LU (2005) In Journal of Pediatric Gastroenterology and Nutrition - Jpgn 41(August). p.195-203
Abstract
Objectives: In mammals, the postnatal development of the

gastrointestinal tract is characterized by vast structural and

functional changes. Using a suckling rat model, we investigated

whether red kidney bean lectin, phytohemagglutinin (PHA),

a potent gut mitogen in adult rats, can accelerate the growth and

maturation of the gastrointestinal tract.

Methods: At either 10 or 14 days of age, suckling rats were daily

gavage fed with PHA (0.05 mg/g body weight) or saline for 3 days.

At 1 or 3 days after this treatment, gastrointestinal organ growth,

intestinal morphology, disaccharidase pattern, macromolecular

absorption capacity, and pancreatic enzyme... (More)
Objectives: In mammals, the postnatal development of the

gastrointestinal tract is characterized by vast structural and

functional changes. Using a suckling rat model, we investigated

whether red kidney bean lectin, phytohemagglutinin (PHA),

a potent gut mitogen in adult rats, can accelerate the growth and

maturation of the gastrointestinal tract.

Methods: At either 10 or 14 days of age, suckling rats were daily

gavage fed with PHA (0.05 mg/g body weight) or saline for 3 days.

At 1 or 3 days after this treatment, gastrointestinal organ growth,

intestinal morphology, disaccharidase pattern, macromolecular

absorption capacity, and pancreatic enzyme contents were studied.

Results: After PHA exposure, increased small intestinal growth

and number of crypt cells were observed, whereas the proportion

of enterocytes with supranuclear vacuoles in the distal

intestine was decreased. The macromolecular absorption of the

markers bovine immunoglobulin (Ig)G and bovine serum

albumin and plasma levels of maternal IgG decreased, and

intestinal disaccharidases switched toward an adult-like pattern.

The pancreas weight and pancreatic protein and trypsin contents

increased. These changes were partly reversible when the PHA

treatment began at 10 days of age, but they persisted when the

treatment began at 14 days of age.

Conclusions: PHA induced enhanced growth and precocious

functional maturation of the gastrointestinal tract in suckling

rats. The effects persisted if the PHA treatment started at 14

days of age, but not before, suggesting an age dependent mechanism.

These findings may lead to a better understanding of

gastrointestinal maturation and constitute a basis for the treatment

of mammals having an immature gut. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Endocytosis, Pancreas, Disaccharidases, Gut, Permeability
in
Journal of Pediatric Gastroenterology and Nutrition - Jpgn
volume
41
issue
August
pages
195 - 203
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000230885000008
  • pmid:16056099
  • scopus:23244456050
ISSN
1536-4801
language
English
LU publication?
yes
id
e6eb3c62-ee54-40c9-a5d2-7dac9d3cda49 (old id 1244631)
alternative location
http://jpgn.org/pt/re/jpgn/pdfhandler.00005176-200508000-00008.pdf
date added to LUP
2008-10-22 15:36:03
date last changed
2017-10-22 03:44:53
@article{e6eb3c62-ee54-40c9-a5d2-7dac9d3cda49,
  abstract     = {Objectives: In mammals, the postnatal development of the<br/><br>
gastrointestinal tract is characterized by vast structural and<br/><br>
functional changes. Using a suckling rat model, we investigated<br/><br>
whether red kidney bean lectin, phytohemagglutinin (PHA),<br/><br>
a potent gut mitogen in adult rats, can accelerate the growth and<br/><br>
maturation of the gastrointestinal tract.<br/><br>
Methods: At either 10 or 14 days of age, suckling rats were daily<br/><br>
gavage fed with PHA (0.05 mg/g body weight) or saline for 3 days.<br/><br>
At 1 or 3 days after this treatment, gastrointestinal organ growth,<br/><br>
intestinal morphology, disaccharidase pattern, macromolecular<br/><br>
absorption capacity, and pancreatic enzyme contents were studied.<br/><br>
Results: After PHA exposure, increased small intestinal growth<br/><br>
and number of crypt cells were observed, whereas the proportion<br/><br>
of enterocytes with supranuclear vacuoles in the distal<br/><br>
intestine was decreased. The macromolecular absorption of the<br/><br>
markers bovine immunoglobulin (Ig)G and bovine serum<br/><br>
albumin and plasma levels of maternal IgG decreased, and<br/><br>
intestinal disaccharidases switched toward an adult-like pattern.<br/><br>
The pancreas weight and pancreatic protein and trypsin contents<br/><br>
increased. These changes were partly reversible when the PHA<br/><br>
treatment began at 10 days of age, but they persisted when the<br/><br>
treatment began at 14 days of age.<br/><br>
Conclusions: PHA induced enhanced growth and precocious<br/><br>
functional maturation of the gastrointestinal tract in suckling<br/><br>
rats. The effects persisted if the PHA treatment started at 14<br/><br>
days of age, but not before, suggesting an age dependent mechanism.<br/><br>
These findings may lead to a better understanding of<br/><br>
gastrointestinal maturation and constitute a basis for the treatment<br/><br>
of mammals having an immature gut.},
  author       = {Linderoth, Ann and Biernat, Marzena and Prykhodko, Olena and Kornilovska, Iryna and Pusztai, Arpad and Pierzynowski, Stefan and Weström, Björn},
  issn         = {1536-4801},
  keyword      = {Endocytosis,Pancreas,Disaccharidases,Gut,Permeability},
  language     = {eng},
  number       = {August},
  pages        = {195--203},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Journal of Pediatric Gastroenterology and Nutrition - Jpgn},
  title        = {Induced Growth and Maturation of the Gastrointestinal Tract After Phaseolus vulgaris Lectin Exposure in Suckling Rats},
  volume       = {41},
  year         = {2005},
}