Characterization of water-soluble hemicelluloses from spruce and aspen employing SEC/MALDI mass spectroscopy
(2002) In Carbohydrate Research 337(8). p.711-717- Abstract
- Partly depolymerized hemicelluloses isolated from wood chips of spruce and aspen employing microwave treatment were resolved using size-exclusion chromatography (SEC) into oligo- and polysaccharide fractions containing components with a narrow range of sizes, as determined by MALDI mass spectroscopy. The degree of substitution with acetyl moieties (DS) was also calculated on the basis of the MALDI-MS spectra obtained prior to and following deacetylation. For spruce hemicelluloses, the low molecular mass fraction contained small arabino-4-O-methylglucuronoxylan oligosaccharides, with DP values ranging from 4 to ~20, separated primarily on the basis of their charge density. The fraction eluted last consisted of an... (More)
- Partly depolymerized hemicelluloses isolated from wood chips of spruce and aspen employing microwave treatment were resolved using size-exclusion chromatography (SEC) into oligo- and polysaccharide fractions containing components with a narrow range of sizes, as determined by MALDI mass spectroscopy. The degree of substitution with acetyl moieties (DS) was also calculated on the basis of the MALDI-MS spectra obtained prior to and following deacetylation. For spruce hemicelluloses, the low molecular mass fraction contained small arabino-4-O-methylglucuronoxylan oligosaccharides, with DP values ranging from 4 to ~20, separated primarily on the basis of their charge density. The fraction eluted last consisted of an O-acetyl-(galacto)glucomannan polysaccharide of peak-average DP value (DPp) 14. The degree of substitution with acetyl groups (DS) decreased with decreasing DP, a value DS of 0.39 being obtained for the fraction with DPp 12. For the aspen hemicelluloses, the SEC fractions eluted first contained an acidic O-acetyl-4-O-methylglucuronoxylan polysaccharide with DP ranging from 10 to ~28 and an average DS of ~0.75. The fractions eluted last consisted of oligosaccharide mixtures composed primarily of small neutral O-acetyl-xylooligosaccharides (DPp 6, DS 0.41), together with minor quantities of an O-acetyl-glucomannan. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/124797
- author
- Jacobs, Anna ; Lundqvist, Jon LU ; Stålbrand, Henrik LU ; Tjerneld, Folke LU and Dahlman, Olof
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aspen wood, Spruce wood, Hemicellulose, O-Acetylation, Mannan, Xylan, Size-exclusion Chromatography, Microwave treatment, MALDI-TOF-MS
- in
- Carbohydrate Research
- volume
- 337
- issue
- 8
- pages
- 711 - 717
- publisher
- Elsevier
- external identifiers
-
- wos:000175193900005
- pmid:11950467
- scopus:0037123263
- ISSN
- 1873-426X
- DOI
- 10.1016/S0008-6215(02)00054-X
- language
- English
- LU publication?
- yes
- id
- b2e7691a-e85d-4ffc-bdbd-6d1104769991 (old id 124797)
- date added to LUP
- 2016-04-01 11:47:51
- date last changed
- 2022-01-26 18:24:31
@article{b2e7691a-e85d-4ffc-bdbd-6d1104769991, abstract = {{Partly depolymerized hemicelluloses isolated from wood chips of spruce and aspen employing microwave treatment were resolved using size-exclusion chromatography (SEC) into oligo- and polysaccharide fractions containing components with a narrow range of sizes, as determined by MALDI mass spectroscopy. The degree of substitution with acetyl moieties (DS) was also calculated on the basis of the MALDI-MS spectra obtained prior to and following deacetylation. For spruce hemicelluloses, the low molecular mass fraction contained small arabino-4-O-methylglucuronoxylan oligosaccharides, with DP values ranging from 4 to ~20, separated primarily on the basis of their charge density. The fraction eluted last consisted of an O-acetyl-(galacto)glucomannan polysaccharide of peak-average DP value (DPp) 14. The degree of substitution with acetyl groups (DS) decreased with decreasing DP, a value DS of 0.39 being obtained for the fraction with DPp 12. For the aspen hemicelluloses, the SEC fractions eluted first contained an acidic O-acetyl-4-O-methylglucuronoxylan polysaccharide with DP ranging from 10 to ~28 and an average DS of ~0.75. The fractions eluted last consisted of oligosaccharide mixtures composed primarily of small neutral O-acetyl-xylooligosaccharides (DPp 6, DS 0.41), together with minor quantities of an O-acetyl-glucomannan.}}, author = {{Jacobs, Anna and Lundqvist, Jon and Stålbrand, Henrik and Tjerneld, Folke and Dahlman, Olof}}, issn = {{1873-426X}}, keywords = {{Aspen wood; Spruce wood; Hemicellulose; O-Acetylation; Mannan; Xylan; Size-exclusion Chromatography; Microwave treatment; MALDI-TOF-MS}}, language = {{eng}}, number = {{8}}, pages = {{711--717}}, publisher = {{Elsevier}}, series = {{Carbohydrate Research}}, title = {{Characterization of water-soluble hemicelluloses from spruce and aspen employing SEC/MALDI mass spectroscopy}}, url = {{http://dx.doi.org/10.1016/S0008-6215(02)00054-X}}, doi = {{10.1016/S0008-6215(02)00054-X}}, volume = {{337}}, year = {{2002}}, }