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Characterization of water-soluble hemicelluloses from spruce and aspen employing SEC/MALDI mass spectroscopy

Jacobs, Anna; Lundqvist, Jon LU ; Stålbrand, Henrik LU ; Tjerneld, Folke LU and Dahlman, Olof (2002) In Carbohydrate Research 337(8). p.711-717
Abstract
Partly depolymerized hemicelluloses isolated from wood chips of spruce and aspen employing microwave treatment were resolved using size-exclusion chromatography (SEC) into oligo- and polysaccharide fractions containing components with a narrow range of sizes, as determined by MALDI mass spectroscopy. The degree of substitution with acetyl moieties (DS) was also calculated on the basis of the MALDI-MS spectra obtained prior to and following deacetylation. For spruce hemicelluloses, the low molecular mass fraction contained small arabino-4-O-methylglucuronoxylan oligosaccharides, with DP values ranging from 4 to ~20, separated primarily on the basis of their charge density. The fraction eluted last consisted of an... (More)
Partly depolymerized hemicelluloses isolated from wood chips of spruce and aspen employing microwave treatment were resolved using size-exclusion chromatography (SEC) into oligo- and polysaccharide fractions containing components with a narrow range of sizes, as determined by MALDI mass spectroscopy. The degree of substitution with acetyl moieties (DS) was also calculated on the basis of the MALDI-MS spectra obtained prior to and following deacetylation. For spruce hemicelluloses, the low molecular mass fraction contained small arabino-4-O-methylglucuronoxylan oligosaccharides, with DP values ranging from 4 to ~20, separated primarily on the basis of their charge density. The fraction eluted last consisted of an O-acetyl-(galacto)glucomannan polysaccharide of peak-average DP value (DPp) 14. The degree of substitution with acetyl groups (DS) decreased with decreasing DP, a value DS of 0.39 being obtained for the fraction with DPp 12. For the aspen hemicelluloses, the SEC fractions eluted first contained an acidic O-acetyl-4-O-methylglucuronoxylan polysaccharide with DP ranging from 10 to ~28 and an average DS of ~0.75. The fractions eluted last consisted of oligosaccharide mixtures composed primarily of small neutral O-acetyl-xylooligosaccharides (DPp 6, DS 0.41), together with minor quantities of an O-acetyl-glucomannan. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aspen wood, Spruce wood, Hemicellulose, O-Acetylation, Mannan, Xylan, Size-exclusion Chromatography, Microwave treatment, MALDI-TOF-MS
in
Carbohydrate Research
volume
337
issue
8
pages
711 - 717
publisher
Elsevier
external identifiers
  • wos:000175193900005
  • pmid:11950467
  • scopus:0037123263
ISSN
1873-426X
DOI
10.1016/S0008-6215(02)00054-X
language
English
LU publication?
yes
id
b2e7691a-e85d-4ffc-bdbd-6d1104769991 (old id 124797)
date added to LUP
2007-07-05 15:10:15
date last changed
2017-10-22 03:38:18
@article{b2e7691a-e85d-4ffc-bdbd-6d1104769991,
  abstract     = {Partly depolymerized hemicelluloses isolated from wood chips of spruce and aspen employing microwave treatment were resolved using size-exclusion chromatography (SEC) into oligo- and polysaccharide fractions containing components with a narrow range of sizes, as determined by MALDI mass spectroscopy. The degree of substitution with acetyl moieties (DS) was also calculated on the basis of the MALDI-MS spectra obtained prior to and following deacetylation. For spruce hemicelluloses, the low molecular mass fraction contained small arabino-4-O-methylglucuronoxylan oligosaccharides, with DP values ranging from 4 to ~20, separated primarily on the basis of their charge density. The fraction eluted last consisted of an O-acetyl-(galacto)glucomannan polysaccharide of peak-average DP value (DPp) 14. The degree of substitution with acetyl groups (DS) decreased with decreasing DP, a value DS of 0.39 being obtained for the fraction with DPp 12. For the aspen hemicelluloses, the SEC fractions eluted first contained an acidic O-acetyl-4-O-methylglucuronoxylan polysaccharide with DP ranging from 10 to ~28 and an average DS of ~0.75. The fractions eluted last consisted of oligosaccharide mixtures composed primarily of small neutral O-acetyl-xylooligosaccharides (DPp 6, DS 0.41), together with minor quantities of an O-acetyl-glucomannan.},
  author       = {Jacobs, Anna and Lundqvist, Jon and Stålbrand, Henrik and Tjerneld, Folke and Dahlman, Olof},
  issn         = {1873-426X},
  keyword      = {Aspen wood,Spruce wood,Hemicellulose,O-Acetylation,Mannan,Xylan,Size-exclusion Chromatography,Microwave treatment,MALDI-TOF-MS},
  language     = {eng},
  number       = {8},
  pages        = {711--717},
  publisher    = {Elsevier},
  series       = {Carbohydrate Research},
  title        = {Characterization of water-soluble hemicelluloses from spruce and aspen employing SEC/MALDI mass spectroscopy},
  url          = {http://dx.doi.org/10.1016/S0008-6215(02)00054-X},
  volume       = {337},
  year         = {2002},
}