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Isolation and characterization of galactoglucomannan from spruce (Picea abies)

Lundqvist, Jon LU ; Teleman, Anita; Junel, Linda; Zacchi, Guido LU ; Dahlman, Olof; Tjerneld, Folke LU and Stålbrand, Henrik LU (2002) In Carbohydrate Polymers 48(1). p.29-39
Abstract
Water-soluble hemicelluloses were extracted from spruce chips by microwave heat fractionation. The chips were impregnated with water at different pH values. Screening of heat-fractionation conditions, i.e. impregnation medium, temperature and residence time was performed with the aim to extract O-acetyl-galactoglucomannan. The impregnation and heat fractionation conditions were evaluated on the basis of the yield of dissolved mannan (oligo- and polysaccharides), molecular weight of the carbohydrates and amount of dissolved lignin. Increasing temperature and residence time increases the yield of mannan and decreases the molecular weight of dissolved carbohydrates. For a structural study of the extracted carbohydrates the chips were... (More)
Water-soluble hemicelluloses were extracted from spruce chips by microwave heat fractionation. The chips were impregnated with water at different pH values. Screening of heat-fractionation conditions, i.e. impregnation medium, temperature and residence time was performed with the aim to extract O-acetyl-galactoglucomannan. The impregnation and heat fractionation conditions were evaluated on the basis of the yield of dissolved mannan (oligo- and polysaccharides), molecular weight of the carbohydrates and amount of dissolved lignin. Increasing temperature and residence time increases the yield of mannan and decreases the molecular weight of dissolved carbohydrates. For a structural study of the extracted carbohydrates the chips were impregnated with water and treated at 200oC for 2min. Oligo- and polysaccharides were fractionated with preparative size-exclusion chromatography from the filtered extract.The structure of the obtained saccharides in two fractions 8 and 9 was determined by 1H NMR spectroscopy. The polysaccharides in the fractions were O-acetyl-galactoglucomannan with a degree of polymerization ~20 and ~11 for fractions 8 and 9, respectively. The molar ratio for galactose:glucose:mannose was approximately 0.1:1:4. About one-third of the d-mannosyl units are substituted by O-acetyl groups almost equally distributed between C-2 and C-3. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Spruce (Picea abies), Hemicellulose, Mannan, O-acetylation, Galactoglucomannan, Microwave oven, Size-exclusion chromatography, NMR spectroscopy
in
Carbohydrate Polymers
volume
48
issue
1
pages
29 - 39
publisher
Elsevier
external identifiers
  • wos:000173328000005
  • scopus:0036532616
ISSN
0144-8617
DOI
10.1016/S0144-8617(01)00210-7
language
English
LU publication?
yes
id
8c9bdb6d-0a07-4704-a6c8-04e1469f77f6 (old id 124831)
date added to LUP
2007-07-06 10:09:35
date last changed
2017-10-22 03:48:39
@article{8c9bdb6d-0a07-4704-a6c8-04e1469f77f6,
  abstract     = {Water-soluble hemicelluloses were extracted from spruce chips by microwave heat fractionation. The chips were impregnated with water at different pH values. Screening of heat-fractionation conditions, i.e. impregnation medium, temperature and residence time was performed with the aim to extract O-acetyl-galactoglucomannan. The impregnation and heat fractionation conditions were evaluated on the basis of the yield of dissolved mannan (oligo- and polysaccharides), molecular weight of the carbohydrates and amount of dissolved lignin. Increasing temperature and residence time increases the yield of mannan and decreases the molecular weight of dissolved carbohydrates. For a structural study of the extracted carbohydrates the chips were impregnated with water and treated at 200oC for 2min. Oligo- and polysaccharides were fractionated with preparative size-exclusion chromatography from the filtered extract.The structure of the obtained saccharides in two fractions 8 and 9 was determined by 1H NMR spectroscopy. The polysaccharides in the fractions were O-acetyl-galactoglucomannan with a degree of polymerization ~20 and ~11 for fractions 8 and 9, respectively. The molar ratio for galactose:glucose:mannose was approximately 0.1:1:4. About one-third of the d-mannosyl units are substituted by O-acetyl groups almost equally distributed between C-2 and C-3.},
  author       = {Lundqvist, Jon and Teleman, Anita and Junel, Linda and Zacchi, Guido and Dahlman, Olof and Tjerneld, Folke and Stålbrand, Henrik},
  issn         = {0144-8617},
  keyword      = {Spruce (Picea abies),Hemicellulose,Mannan,O-acetylation,Galactoglucomannan,Microwave oven,Size-exclusion chromatography,NMR spectroscopy},
  language     = {eng},
  number       = {1},
  pages        = {29--39},
  publisher    = {Elsevier},
  series       = {Carbohydrate Polymers},
  title        = {Isolation and characterization of galactoglucomannan from spruce (Picea abies)},
  url          = {http://dx.doi.org/10.1016/S0144-8617(01)00210-7},
  volume       = {48},
  year         = {2002},
}